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Shahin Khan ¹, Ajay Kumar Meena ², Ajay Saluja ¹

Affiliations
1 A. R. College of Pharmacy & G. H. Patel Institute of Pharmacy, Anand, IN
2 Central Council for Research in Ayurvedic Science, Janakpuri, New Delhi, IN

Abstract

Ayurveda has abundant collection of effective formulations against diseases. The polyherbal formulation contains Vernonia anthelmintica, Curcuma longa, Piper longum, Mallotus philippinensis, Operculina turpethum, Terminalia chebula, Butea monosperma, and Red ochre. The present study deals with anthelmintic evaluation of ayurvedic formulation. Various concentrations of polyherbal formulation (10 mg/ml, 20 mg/ml and 30 mg/ml) in 50 ml of normal saline were taken. The results were compared with standard albendazole (10mg/ml, 20mg/ml and 30 mg/ml) and control was taken as normal saline (0.9% NaCl). The results were expressed in terms of time in minutes for the paralysis and time of death of the worms. Paralysis and death time were analyzed using one way ANOVA analysis using Graph pad prism 2.01 Software. The study indicates that polyherbal formulation shows more potent anthelmintic activity than standard reference.

Keywords

Polyherbal Formulation, Krumighattini Tablet, Anthelmintic Activity, Albendazole.

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Nidhi Dubey ¹, Nitin Dubey ², Rajendra Mehta ³, Ajay Saluja ³

Affiliations
1 School of Pharmacy, Devi Ahilya Vishwavidyalaya, Indore [M.P], IN
2 College of Pharmacy, IPS Academy, Indore [M.P], IN
3 A. R. College of Pharmacy, Vallabh Vidyanagar [Gujarat], IN

Abstract

A simple, sensitive, rapid and selective high-performance liquid chromatographic (HPLC) method has been developed and validated for the analysis of catechin in marketed Ayurvedic Taila (oil) formulations containing Acacia catechu. Chromatography of methanolic: 0.1% formic acid (7:3, v/v) extracts of these formulations was performed on C₁₈ (5 μm×25 cm×4.6 mm i.d) column using isocratic mobile phase consisting of methanol:acetonitrile:water (40:15:45, v/v/v) containing 1.0% acetic acid at a flow rate of 0.5 ml/min and SPD-10 A_VP photodiode array (PDA) UV-Visible detector. The analytical marker, catechin, was quantified at 279 nm. The retention time of catechin was about 3.27 min. The linear regression analysis data for the calibration plot showed a good linear relationship with correlation coefficient of 0.9988 in the concentration range of 15 to 90 μg/ml for catechin with respect to peak area. The limit of detection and limit of quantitation values were found to be 0.5 μg/ml and 1.7 μg/ml respectively. Repeatability of the method was found to be 0.62 RSD. Recovery values from 99.73 to 100.22% indicate excellent accuracy of the method. The developed HPLC method is accurate, precise, and cost-effective, and it can be successfully applied for the determination of catechin in marketed ayurvedic oil formulations containing Acacia catechu.

Keywords

Acacia catechu, HPLC, Oil, Quantitation.

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