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Kumari, Anuradha
- Infectious Bursal Disease Standardization and Seroprevalence Study in Ranchi in Poultry
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Authors
Affiliations
1 Division of Microbiology, Ranchi Veterinary College, Birsa Agricultural University, Kanke, RANCHI (JHARKHAND), IN
1 Division of Microbiology, Ranchi Veterinary College, Birsa Agricultural University, Kanke, RANCHI (JHARKHAND), IN
Source
Research Journal of Animal Husbandry & Dairy Science, Vol 6, No 2 (2015), Pagination: 109-114Abstract
The work was planned to develop diagnostic assay using hyper immune sera and poultry sera with seroprevalence monitoring in Ranchi (Jharkhand). The best result for dot-ELISA was standardized with 1.2 μl of BursaB2K and Gumboro strain, skimmed milk 1.5 per cent+gelatin 0.5 per cent+BSA 1 per cent as blocking solution, sera dilution of 1:10 and 1:500 conjugate dilution. Statistical analyses were showing there is a non-significant difference in results of dot-ELISA and ELISA test to detect specific anti IBDV antibodies. In the present study, dot-ELISA based seroprevalence study of 92 suspected poultry samples in 18 different places of Ranchi in the year 2014-15, revealed IBD antibodies existed in 38.04 per cent with sensitivity and specificity of 67.57 per cent and 81.82 per cent, respectively. Serodiagnosis helps in monitoring immune status of poultry flock in the area for IBD. There was large variation in IBD positive antibodies in different places of Ranchi, ranging between 14.29 per cent to 100 per cent by dot-ELISA. Similar degree of results were observed with two strains of live attenuated IBD antigen i.e. Bursa B2K (invasive intermediate) and Gumboro (intermediate) strain. Statistical analysis revealed non-significant difference between dot - ELISA and ELISA. Dot-ELISA can be taken as promising tool in field.Keywords
Dot-ELISA, IBD, Seroprevalence, Poultry.
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- Effect of Antioxidants Supplementation on the Quality of Beetal Buck Semen Stored at 4°C
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Materials and Methods: Thirty-six semen ejaculates were collected by artificial vagina from 6 bucks (Beetal) during the normal reproduction season (September to November) at weekly interval. The samples were centrifuged, and the seminal plasma was removed. The sperm pellet was diluted with Tris-based extender and divided into three groups. Group T1:Control samples without antioxidants, Group T2:Samples supplemented with tocopherol at 3 mM, and Group T3:Samples supplemented with glutathione at 1 mM. The samples were evaluated for progressive motility, percent liveability, percent abnormal spermatozoa, and acrosome integrity after liquid preservation for 0, 24, 48, and 72 h. The level of lipid peroxidation and antioxidant enzymes, namely, glutathione peroxidase (GPx) and superoxide dismutase (SOD) were estimated after liquid preservation for 0 and 72 h.
Results: It was observed that, after storage of semen at 4°C up to 72 h, the progressive sperm motility, percent liveability, percent abnormal spermatozoa, and percent intact acrosomes were significantly (p˂0.05) higher in group T2 and T3 samples as compared to control. However, the level of lipid peroxidation in T2 and T3 groups was significantly (p˂0.05) lower after 72 h of incubation at 4°C. Similarly, GPx and SOD values were significantly (p˂0.05) increased in T2 and T3 groups after 72 h of storage at 4°C.
Conclusion: Thus, it can be concluded that Vitamin E and glutathione supplementation at 3 mM and 1 mM, respectively, while preserving the semen samples at 4°C helped in maintaining the seminal parameters up to 72 h and protected the spermatozoa from oxidative damage.
Authors
Archana Sarangi
1,
Pardeep Singh
2,
Meenakshi Virmani
2,
A. S. Yadav
3,
Subhasish Sahu
4,
H. M. Ajithakumar
1,
Anuradha Kumari
4,
A. P. Rath
5
Affiliations
1 Division of Animal Physiology, ICAR-National Dairy Research Institute, Karnal, Haryana, IN
2 Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
3 Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
4 Department of Livestock Production and Management, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
5 Department of Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
1 Division of Animal Physiology, ICAR-National Dairy Research Institute, Karnal, Haryana, IN
2 Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
3 Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
4 Department of Livestock Production and Management, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
5 Department of Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, IN
Source
Veterinary World, Vol 10, No 10 (2017), Pagination: 1184-1188Abstract
Aim: An experiment was designed to evaluate the role of Vitamin E and glutathione in improving the seminal parameters during hypothermic storage of liquid semen at 4°C for 72 h.Materials and Methods: Thirty-six semen ejaculates were collected by artificial vagina from 6 bucks (Beetal) during the normal reproduction season (September to November) at weekly interval. The samples were centrifuged, and the seminal plasma was removed. The sperm pellet was diluted with Tris-based extender and divided into three groups. Group T1:Control samples without antioxidants, Group T2:Samples supplemented with tocopherol at 3 mM, and Group T3:Samples supplemented with glutathione at 1 mM. The samples were evaluated for progressive motility, percent liveability, percent abnormal spermatozoa, and acrosome integrity after liquid preservation for 0, 24, 48, and 72 h. The level of lipid peroxidation and antioxidant enzymes, namely, glutathione peroxidase (GPx) and superoxide dismutase (SOD) were estimated after liquid preservation for 0 and 72 h.
Results: It was observed that, after storage of semen at 4°C up to 72 h, the progressive sperm motility, percent liveability, percent abnormal spermatozoa, and percent intact acrosomes were significantly (p˂0.05) higher in group T2 and T3 samples as compared to control. However, the level of lipid peroxidation in T2 and T3 groups was significantly (p˂0.05) lower after 72 h of incubation at 4°C. Similarly, GPx and SOD values were significantly (p˂0.05) increased in T2 and T3 groups after 72 h of storage at 4°C.
Conclusion: Thus, it can be concluded that Vitamin E and glutathione supplementation at 3 mM and 1 mM, respectively, while preserving the semen samples at 4°C helped in maintaining the seminal parameters up to 72 h and protected the spermatozoa from oxidative damage.
Keywords
Beetal Buck, Glutathione and Liquid Preservation, Oxidative Stress, Semen, Seminal Parameters, Vitamin E.Full Text