Informatics Publishing Limited

N. Shahi ¹, S. K. Mallik ¹, D. Sarma ¹

Affiliations
1 Directorate of Coldwater Fisheries Research (ICAR), Bhimtal 263 136, Nainital, (UK), IN

Abstract

Total and differential leukocyte count, and the phagocytic activity in blood of common carp, Cyprinus carpio (Linnaeus, 1758), experimentally infected with virulent Aeromonas allosaccharophila was evaluated in this study. For experimental study, common carp fingerlings (10.0 ± 1.5 g Weight, 8.5 ± 2.3 cm Length) were divided into five groups in duplicates of un-injected fish, fish injected with sterile phosphate buffered saline (PBS), and fish injected with 4.2 x 104, 1.9 x 106 and 7.3 x 108 colony forming units per milliliter (CFU ml-1) of suspension of A. allosaccharophila. Forty eight hours after infection, the total and differential leukocyte count and phagocytic activity of blood leukocytes of common carp was determined. The leukocyte, lymphocyte and monocyte count were significantly (P< 0.05) high in experimentally infected fishes, while no significant change in neutrophils number was observed. In fishes injected with 4.2 x 104, 1.9 x 106 and 7.3 x 108 CFU ml-1 of bacterial suspension, the percentage of phagocytic activity in the blood were 55.3, 54.7 and 58.2, respectively. The result suggests that, A. allosaccharophila could influence the leukocyte number and phagocytic activity of blood of common carp, which is an integral component of innate immune system of fish.

Keywords

Bacteria, Blood, Fish, Innate Immunity, Phagocytosis.

Full Text

   

N. Shahi ¹, S. K. Mallik ¹, D. Sarma ¹, M. Sahoo ², P. Das ¹

Affiliations
1 Directorate of Coldwater Fisheries Research, (ICAR) Industrial Area, Bhimtal, 263 136, Nainital, (Uttarakhand), IN
2 Indian Veterinary Research Institute, Izatnagar, Bareilly, (UP), IN

Abstract

Corneal hypertrophy was observed in fingerlings of chocolate mahseer, Neolissochilus hexagonolepis (McClelland, 1839) from Uttarakhand, India. The advanced stage of infection was marked with damage of cornea and shrunken eye. Skeletal deformity was also recorded in some of the infected fishes. Samples of eye and other organ were collected for histopathological, parasite and microbial investigation. Histological examination of the eye indicated ocular collapse, entirely damaged cornea, disruption of vitreous, aphakic globe, oedema of choroid and optic nerve and, deformed retina. Microscopic observations of wet mounts and histopathology ruled out infectious agents like microsporidia, myxosporidea, cystercoidiasis, atypical mycobacteria, digeneans and fungi. PCR for viral aetiology did not yield any significant amplicons. Further detail study related to physic-chemical parameter need to be carried out to ascertain the exact cause of this disease.

Keywords

neolissochilus Hexagonolepis, Pathology, Eye, Corneal Hypertrophy, Chocolate Mahseer.

Full Text

   

N. Shahi ¹, J. Pandey ¹, S. K. Mallik ¹, D. Sarma ¹, P. Das ¹

Affiliations
1 Industrial Area, Bhimtal, Uttarakhand-263 136, IN

Abstract

Golden mahseer, Tor putitora is an endangered fish of mid-Himalayan regions of India. It has good potential as aquaculture species for hill aquaculture, due to its high market demand. But the major constrains on introduction of this fish for aquaculture is its inability to breed in captivity and slow growth rate. Therefore, studies were carried out on its breeding biology to captive breed this fish for sustainable seed production. However, detailed study on reproductive pattern of adult golden mahseer is very limited. Therefore, in the present study, we have developed a macroscopic grading system for golden mahseer testes, which shows the testes development stages during the breeding season, which lasts from May-September in Bhimtal and Sattal lakes. The golden mahseer were sampled from February - September 2014, from Bhimtal and Sattal lakes. Altogether, 48 samples were collected during the sampling period. Based on macroscopic observation, the maturation stage of the testes was divided into five developmental stages: Immature (I), early spermatogenic (II), late spermatogenic (III), matured (IV) and spent (V).

Keywords

Tor Putitora, Reproduction, Maturity, Testes.

Full Text

   

H. K. Kalita ¹, B. Talukdar ¹, R. A. Baishya ¹, S. Basumatary ¹, J. Das ², A. Dutta ¹, R. Ch. Barman ³, D. Sarma ¹

Affiliations
1 Department of Zoology, Gauhati University, Guwahati, Assam, IN
2 Department of Zoology, Goalpara College, Goalpara, Assam, IN
3 NFDB Regional Centre, Campus of NIRD, NERC, Khanapara, Guwahati, IN

Abstract

The study was conducted in a society pond of Gandhibasti village of Abhayapuri, Bongaigaon, Assam to assess the impact of duck droppings on water quality and pond productivity. The pond was integrated with ducks (Anas platyrhynchos) to obtain the benefit of duck litters on the pond productivity and fish growth. The mean dry weight of 80 ducks excreta was estimated as 3.864 kg/ha/day, discharging into the pond. 18,000 fingerlings. Indian major carps along with few exotic carp were stocked in the month of April, 2013. Supplementary feed was also given to the fishes in alternate days to ensure their steady growth. All the studied physico-chemical parameters were found within the permissible limit and the growth of fishes was also satisfactory. The maximum growth was recorded in Catla followed by silver carp, common carp, Rohu and Mrigala.

Keywords

Duck Dropping, Pond Productivity, Indian Major Carps, Integrated Farming.

Full Text

   

D. Sarma ¹, J. Das ¹, A. Dutta ¹

Affiliations
1 Department of Zoology, Gauhati University, Guwahati-781 014, Assam, IN

Abstract

Rogor is a commonly used pesticide in the agricultural field. The present study was undertaken to evaluate the lethal toxicity of Rogor, an organophosphorus pesticide, in freshwater fish Channa punctatus (Bloch) and relative behavioural changes after exposing for 96 hours. The regression equation calculated as y = 9.551x + 6.173 and the LC₅₀ value calculated for Channa punctatus was at 0.75 mL/L. During exposure, fish showed faster opercular activity, erecting dorsal and ventral fin, huge secretion of mucus from whole body, etc.

Keywords

Acute Toxicity, Channa punctatus, Rogor, LC₅₀.

Full Text

   

D. Sarma ¹, A. Dutta ²

Affiliations
1 Department of Zoology, Goalpara College, Goalpara-783 101, Assam, IN
2 Department of Zoology, Gauhati University, Guwahati, Assam, IN

Abstract

The ecology in terms of physico-chemical and biological properties of Urpod beel and Kumri beel of Goalpara district of Assam were assessed from 2001 to 2003. Physico-chemical properties of both the wetlands were observed favourable. Biological properties such as plankton population and primary productivity fluctuated within the permissible limits. However, eutrophication tendency appeared in both the wetlands due to autochthonous and allochthonous input.

Keywords

Goalpara, Riverine Wetlands, Urpod and Kumri Beels, Ecological Studies.

Full Text