Informatics Publishing Limited

Shaza Anwar Al Laham , Frdoos Mohammad Al Fadel

Abstract

Streptococcus is considered to be one of the most dangerous causes of Clinical mastitis in cows. The aim of this study was to investigate the effectiveness of extracts prepared from different parts of the flowing plants: Oleaeuropea Linn(Oleaceae), Myrtuscommunis Linn (Liliaceae) ,thymus vulgaris Linn (Laminaceae), Rosemery Linn(Laminaceae), Ficuscarica Linn (Moraceae), and Achilleafalcata Linn(Asteraceae) against resistant Streptococcus in 1371 Samples of milk. This work was achieved in four stages: First of all, the presence of Streptococcus in 1371 Samples of milk, by using blood agar, Bile Esculin agar, and some bio-chemical tests were investigated .Secondly, the antibacterial activity of many antibiotics on these bacteria by using disc diffusion method were determined.Thirdly, the plants were extracted with water, absolute alcohol, and petroleum Ether by using soxhlet apparatus and rotary vacuum evaporator. Fourthly, the antibacterial activity of the extractions on resistant Streptococcus was determined by using disc diffusion method. This study has shown the presence of different antibacterial effectiveness of the extracts prepared from different parts of those plants. The extract of thymus vulgaris is more effective when compared to the extract of Oleaeur opaea against resistant Streptococcus.

 

Keywords

Oleaeuropea , Myrtuscommunis, thymus vulgaris , Rosemery , Ficuscarica ,Achilleafalcata, mastitis, Streptococcus.

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Shaza Anwar Al Laham , Frdoos Mohammad Al Fadel

Abstract

The increasing number of Escherichia coli causing mastitis and of bacteria resistant to conventional antibiotics has become a serious problem in recent years. So the search for new antibiotics and alternative products to solve this problem is the question of the age. This research aims to investigate the effectiveness of the extracts prepared from different parts of the following plants: Olea europea Linn ( O leaceae ), Myrtus communis Linn (Liliaceae ), Majorana syriacus Linn (Laminaceae ), Zingiber officinale Linn ( Zingiberaceae ), Achillea falcata Linn ( Asteraceae) against resistant Escherichia coli Type (1). Investigation began for E.coli bacteria in 667 milk samples. The bacteria were identified culturally, morphologically and biochemically. Antibiotic susceptibility testing against E.coli by Kirby - Bauer disk diffusion method were conducted. Then using the blood agar, MacConkey agar, salmonella - shigella agar, and biochemical testing method [API 20 E testing Enterobacteriaceae] were made to type E.coli . P lants were extracted with water , absolute alcohol, then either using a soxhlet apparatus and rotary vacuum evaporator. T he n extracts susceptibility testing against antibiotic resistant E.coli Type (1) were studied. E.coli was defined as oxidase negative, indole positive, catalase positive. The studied antibiotics did not show any antibacterial effect against E.coli. By t he results of the biochemical analysis (API20e) on resistant E.coli, E.coli type (1) was 33.35% of the total number of samples. The anti - bacterial effectiveness against E.coli type (1) of ethanol extracts prepared from different parts of the studied plants were variant, whereas the Myrtus communis extract effectively has the most powerful antibacterial effect for these bacteria , while the Zingiber officinale extract has the lowest influence.

Keywords

Olea europea, Myrtus communis , Achillea falcata, Majorana syriacus, Zingiber officinale, E.coli, Resistant Bacteria.

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