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G. Senthilkumar ¹, A. Josphine ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 Dept. of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt.) - 613 503, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Elambalur, Perambalur - 621 212, Tamil Nadu, IN

Abstract

The marine fungi occurring on the wood samples were studied. Diversity of marine fungi colonizing the lignicolous wood samples was assessed and their distribution pattern was determined. The antibacterial activity of Aspergillus oryzae was tested against five human pathogens by agar well diffusion method. Based on the results, the compounds were separated by Thin Layer Chromatography (TLC) and these compounds were tested against three human bacterial pathogens. The separated compounds were further analyzed by Fourier Transform Infrared Spectroscopy (FTIR) to identify the functional groups, for the antibacterial activity.

Keywords

Aspergillus oryzae, FTIR, TLC, Sterol.

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S. Suganya ¹, R. Bharathidasan ¹, G. Senthilkumar ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

Extraction of essential oils from locally available plant Coriandrum sativam (L.) was carried out using steam distillation followed by ether extraction. Dried and purified extracted oils were screened for their antibacterial activity of both gram positive (Staphylococcus aureus) and gram negative (Enterobacter aerogenes, Klebsiella pneumoniae, Vibrio cholerae and Salmonella typhi) bacterial strains by Agar well diffusion method. Coriandrum sativam shows antibacterial activity against the above given five bacterial strains. GC-MS was done on Coriandrum sativam oil. These results support that this plant oil can be used to cure bacterial infections and may also have role as pharmaceuticals and preservatives.

Keywords

Coriandrum sativam, GC-MS, Essential Oil, Antibacterial Activity and Extraction.

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A. Josphine ¹, G. Senthilkumar ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College [Autonomous], Poondi-Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

The variety and galaxy of fungi and their natural beauty occupy prime place in the biological world and India has been the cradle for such fungi. Only a fraction of total fungal wealth has been subjected to scientific scrutiny and mycologist have to unravel the unexplored and hidden wealth. The marine fungi occurring on the wood samples collected from the inter tidal region of the Muthupet mangrove environment were studied. Diversity and distribution of different organisms in the marine environment are influenced by the physico-chemical properties of the soil. Totally 23 fungal species were recorded.

Keywords

Fungi, Diversity, Wood Samples, Mangroves.

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G. Senthilkumar ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 Dept of Botany and Microbiology, A. V. V. M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) - 613 503, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

The chromosomal DNA was isolated from T. harzianum and PCR amplification was performed. The 18S rDNA gene sequencing was made by using ITS (internal transcribed spacer) method. The 18S rDNA gene sequences were submitted to Genebank, NCBI, the accession number GU646678 has been obtained. The phylogenetic tree was constructed using MEGA 4 (Molecular Evolutionary Genetics Analysis Software version 4.0). Based on the neighbour joining tree analysis the isolate T. harzianum has been found closely related to already existing species of Hypocera tixii at 99 per cent of similarity.

Keywords

Trichoderma harzianum, DNA Isolation, Evolution.

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G. Senthilkumar ¹, P. Madhanraj ¹, A. Panneerselvam ¹

Affiliations
1 Dept of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur (Dt), Tamil Nadu, IN

Abstract

Totally 42 species belonged to 20 genera were recorded. A preliminary screening of all the species isolated from soils were made for antifungal (antagonistic) activity against Fusarium oxysporum, a known soil borne fungal pathogen. Among the species tested the Trichoderma harzianum inhibited the pathogenic fungus to the maximum both in dual culture and in food poisoning technique. Gas chromatography mass spectrum analysis of acetonitrile extract of the filtrate of T. harzianum revealed the presence of six compounds represents six major peaks. The peaks correspond with diethyl phthalate, tetradecanoic acid 9,12-octadecadienoic acid (z,z), oleic acid, 1, 2-benzene di oxylic acid, diisooctyl ester and squalene.

Keywords

Fungal Extract, Compounds, GC-MS.

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