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Mahantesh Y. Jogi ¹, M. B. Madalageri ¹, V. M. Ganiger ¹, G. Bhuvaneswari ², H. B. Patil ¹, Y. K. Kotikal ¹

Affiliations
1 Department of Vegetable Science, College of Horticulture, Bagalkot, Karnataka, IN
2 Department of Vegetable Science, College of Horticulture, Bagalkot, Karnataka, GH

Abstract

Character association and path analysis in fifty genetically diverse indigenous and exotic genotypes of chilli was studied for 16 important characters. The phenotypic and genotypic association of fruit yield was significantly positive with all the characters except days to first flowering and ten fruit weight. Early fruit yield and late fruit yield per plant were found highly significant and positive correlation with total fruit yield. The genotypic path co-efficient analysis revealed that ascorbic acid and chlorophyll content had high direct positive effect on total fruit yield.

Keywords

Chilli, Correlation, Path Analysis, Total Yield

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S. N. Malaghan ¹, M. B. Madalageri ², V. M. Ganiger ¹, G. Bhuvaneshwari ³, Y. K. Kotikal ⁴, H. B. Patil ¹

Affiliations
1 Department of Vegetable Science, College of Horticulture, Bagalkot, Karnataka, IN
2 Directorate of Research, University of Horticultural Sciences, Bagalkot, Karnataka, IN
3 Department of Foods and Nutrition, College of Horticulture, Bagalkot, Karnataka, IN
4 Directorate of Extension, University of Horticultural Sciences, Bagalkot, Karnataka, IN

Abstract

An investigation was carried out at College of Horticulture, University of Horticultural Sciences, Bagalkot during summer 2011 to analyze the variability and heritability in 67 cluster bean genotypes for vegetable pod yield and its growth and yield parameters. All the characters showed highly significant (at P=0.01) difference. Maximum range of variability was observed for ten fresh pod weight (g), number of branches at 90 DAS and number of dry pod per plant (dual purpose type). High heritability coupled with high genetic advance over mean was observed for number of branches at 90 DAS, pod length (cm), and ten fresh pod weight (g). The results of present investigation suggested that selection based on number of dry pod per plant, fresh pod weight, pod length and number of branches at 90 DAS might bring improvement in vegetable pod yield of cluster bean and its growth and yield components characters.

Keywords

Clusterbean, Genotypic Co-efficient, Genetic Advance, Heritability, Phenotypic Co-efficient of Variance

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Mahantesh Y. Jogi ¹, M. B. Madalageri ², G. Bhuvaneswari ³, H. B. Patil ¹, Y. K. Kotikal ⁴

Affiliations
1 Department of Vegetable Science, College of Horticulture, Bagalkot, Karnataka, IN
2 Directorate of Research, University of Horticultural Sciences, Bagalkot, Karnataka, IN
3 Department of Foods and Nutrition, College of Horticulture, Bagalkot, Karnataka, IN
4 Directorate of Extension, University of Horticultural Sciences, Bagalkot, Karnataka, IN

Abstract

Genetic variability, heritability, genetic advance and genetic advance as a per cent over mean for thirty two characters were assessed by field evaluation of fifty chilli accessions at College of Horticulture, Bagalkot (Karnataka) during 2010-11. High degree of variation was observed for all characters. The difference between phenotypic co-efficient of variation and genotypic co-efficient of variation were found to be narrow for most of the traits. The high estimates of heritability was found for plant spread (N-S) at 60 days (64.27%) and 120 day (65.47%) and (E-W) at 60 days (60.56%), number of fruits per plant at first picking (98.20%), early yield (94.67%), late yield (95.62%) and total yield (91.37%). The fruit characters like fruit width (96.22%), stalk length (81.04%) and ten fruit weight (96.44%), chlorophyll-a (95.45%), chlorophyll-b (97.52%), total chlorophyll (97.87%) and ascorbic acid (98.30%), fresh red chilli yield (95.18%) and dry red chilli yield (93.71%), hundred seed weight (70.67%) and number of seeds per fruit (94.67%) had also recorded high heritability.

Keywords

Green Chilli, Genetic Advance, Heritability, Genotypic Variance, Phenotypic Variance

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V. D. Deshmukh ¹, H. B. Patil ², J. D. Deshmukh ³, I. A. Madrap ⁴

Affiliations
1 MGM College of Agricultural Biotechnology, Aurangabad (M.S.), IN
2 College of Agriculture Biotechnology, Latur (M.S.), IN
3 Banana Research Station, Nanded (M.S.), IN
4 Department of Botany, Marathwada Agricultural University, Parbhani (M.S.), IN

Abstract

The present study was carried out with emphasis on ISSR profiling and genetic relationship studies on pearl millet using ISSR marker. The experimental material consisted of 15 genotypes of pearl millet. The seeds of 15 genotypes of pearl millet were grown in petriplates and the young leaves from them were used for DNA extraction. Extraction of total genomic DNA was carried out following the methods suggested by Saghai-Maroof et al. (1984) with minor modification. The selected primers generated 123 ISSR bands and the size of the amplification products ranged from 150-35000 bp. The number of bands generated per primer ranged from 09 (CA)₆RG to 19 (AC)₈YA with a mean of 13.6 bands per primer. Out of the bands generated 80 were polymorphic and 43 were monomorphic. The genotype GHB 235 showed maximum similarity (0.92) whereas, minimum was for ICMV 155 (0.55) among the genotypes studied.

Keywords

Pearl Millet, Genetic Diversity, ISSR.

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V. N. Patil ¹, B. C. Chaudhari ¹, H. B. Patil ¹

Affiliations
1 Department of Soil Science and Agricultural Chemistry, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN

Abstract

Field experiment as conducted in 1999-2000 at CRS farm Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. The experiment was laid out in Factorial Randomized Block Design with four treatment replicated four times. The uptake of nutrient was higher for sucker grown plants compared with tissue culture plants due to greater accumulation of dry matter by the former. The spacing had no significant effect nutrient uptake by banana plants.

Keywords

Tissue Culture, Banana Nutrient Uptake.

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V. N. Patil ¹, B. C. Chaudhari ¹, H. B. Patil ¹

Affiliations
1 Department of Soil Science and Agricultural Chemistry, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN

Abstract

Field experiment was conducted in 1999-2000 at CRS farm Dr. PDKV, Akola. The experiment was laid out in Factorial Randomized Block Design with four treatment replicated four times. It was observed that tissue culture developed plants had given good quality fruits, with significantly more non-reducing sugar and total sugar content as compared to sucker grown plants of banana. However, the chlorophyll, calcium and ascorbic acid content of banana fruit were not significantly affected by method of propagation. Similarly, spacing had no significant effect on quality of fruits.

Keywords

Tissue Culture, Banana, Chlorophyll, Reducing Sugar, Non Reducing Sugar, Total Sugar.

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R. L. Chavhan ¹, V. Y. Patade ², H. B. Patil ³

Affiliations
1 Central Institute for Cotton Research, Post Bag No. 2, Shankarnagar P.O., Nagpur (M.S.), IN
2 Green House Laboratory, Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, BARC, Trombay, Mumbai (M.S.), IN
3 Department of Agricultural Biotechnology, Marathwada Agricultural University, Parbhani (M.S.), IN

Abstract

Callus induction and regeneration in safflower (Carthamus tinctorius L.) cv. Sharda and PBN-12 was optimized using various explants such as hypocotyls, cotyledonary leaves ischolar_mains and apical shoot buds. The cotyledonary leaves exhibited better response for callus induction as compare to ischolar_mains and hypocotyls on MS supplemented with 5mgl^-1 NAA, 0.25mg^-1 BAP and 1% sucrose.

Keywords

Callus Induction, Plantlet Regeneration, Safflower.

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K. J. Wable ¹, H. B. Patil ¹, S. G. Rajput ¹

Affiliations
1 Department of Agril. Biotechnology, Marathwada, Agriculture University Parbhani - 431 402 (M.S.), IN

Abstract

The importance of loading and treatment with a vitrification solution on the survival of Citrus reticulata embryonic axes cryopreserved using vitrification technique (Derudde et al., 1990) were investigated in this paper. Loading treatment was carried out for 20 min. at 25 0C before chemical dehydration with 100 per cent PVS2 for 20 min. After treatment with PVS2 rapid immersion in liquid nitrogen, rapid rewarming, unloading in a 1.2 M sucrose solution for 20 min. Then the culture on solid medium with 0.3 M sucrose for 1 day and growth recovery for 4 weeks on standard medium, survival of C. reticulata embryonic axes reached 60 per cent following the process.

Keywords

Citrus, Vitrification, Cryopreservation.

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