Informatics Publishing Limited

Jayshree Pawar ¹, Sandeep Sonawane ¹, Santosh Chhajed ¹, Sanjay Kshirsagar ¹

Affiliations
1 Department of Pharmaceutical Analysis, MET’s Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik – 422 003, IN

Abstract

A new, simple and validated RP-HPLC method was developed for simultaneous estimation of MetforminHCl (MET) and Gliclazide (GLZ).The separation was achieved by using mobile phase Methanol: 10 mM Phosphate buffer (pH 3) 70:30 % v/v on Kinetics C₁₈ column (250 x 4.6 mm, 5μ). The flow rate was 1 ml/min. The detection was carried out at wavelength 230 nm.The retention time of MET and GLZ was found to be 2.20 min and 4.67 min, respectively. Calibration curves for MET and GLZ were found to be linear in the range of 10-60 μg/ml and 2-12 μg/ml, respectively. The proposed method was validated as per ICH guideline Q2 (R1). The validation parameters studied were linearity, accuracy, precision, specificity and robustness. All validation parameters were found within the acceptable range.

Keywords

Metformin HCl, Gliclazide, RP-HPLC, Analytical Method Validation.

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Jayshree Pawar ¹

Affiliations
1 University of Pune, Pune, IN

Abstract

This paper tries to understand price movements of selected commodities in urban and rural area of Amravati district. It is observed that the average prices of commodities undergo fluctuations over the years where price movement of all commodities varies from each other depending upon the nature of commodity and its market. It is also observed that rural prices are higher than urban prices. It questions the belief of low cost of living in rural areas excluding housing and other social infrastructure.

Keywords

Inflation Rate, Urban Areas, Rural Areas.

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