Informatics Publishing Limited

K. Kaviyarasi ¹, A. Cholarajan ², R. Bharathidasan ², P. Madhanraj ², A. Panneerselvam ²

Affiliations
1 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
2 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN

Abstract

PGPR offers an environmentally sustainable approach to crop production and health. Many members of genera pseudomonas sp. perform beneficial effects on plants. Among them P. putida and P. fluorescens are fluorescent pseudomonas that represent Significant part of these Members. The rhizosphere soil was collected from cultivated plots. P. fluorescence was isolated and identified through morphologicaland hemical tests and it was treated against some plant pathogens. The biocontrol potential of P. fluorescens against plant pathogen such as A. terreus sp, Circinella sp, Verticillium sp, was assessed by dual plate technique. The seed treatment was made on four plants namely Hibiscus esculentus, Oryza sativa, Capsicum annum, Spinacia olerace. Pot culture techniques was applied for the growth of plants along with p. fluorescence inoculums (100ml) and chemical fertilizer (10g).The plant height, ischolar_main length, ischolar_main biomass, shoot biomass were also estimated. The results were discussed.

Keywords

PGPR, Pseudomonas fluorescence.

Full Text

     

K. Kaviyarasi ¹, K. Kanimozhi ¹, P. Madhanraj ², A. Panneerselvam ¹, V. Ambikapathy ¹

Affiliations
1 Department of Botany and Microbiology, A.V.V.M .Sri Puspham College [Autonomous], Poondi-613 03, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621 212, Tamil Nadu, IN

Abstract

The soil sample was collected from Manora. After analysing the physic-chemical parameters of the collected marine soil sample, ten actinomycetes species including Actinobispora yunnanensis, Streptomyces albus,Micromonospora echinospora, Saccharopolyspora hirsute, Streptomycetes cyaneus, Actinomadura citrea, Saccharomonospora viridis, Thermomonospora mesophila, Streptoverticillium album Microtetrospora fastidiosa were isolated and they were screened for phosphate solubilization using Pikovskaya's agar medium and the Actinobispora yunnanensis was found to be dominant in phosphate solubilization. Then the DNA of the Actinobispora yunnanensis was isolated, amplified and its 16S rDNA was sequenced and its secondary structure was also predicted.The phylogenetic analysis of the Actinobispora yunnanensis was also carried out.

Keywords

Phosphate, Phosphate Solubilization, 16S r DNA, Actinobispora yunnanensis.

Full Text