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Gamit, Kanan
- A Pilot Study on Evaluation of Standardized Ayurveda formulation Ashwagandhadi lehya as Aphrodisiac and in Treatment of Oligospermia
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Authors
Affiliations
1 Pharmacognosy and Phytochemistry Department, Ramanbhai Patel College of Pharmacy, Charusat, At and Post – Changa, Dist. Anand, Gujarat, 388421, IN
2 J.S. Ayurveda College, College Road, Nadiad, Gujarat, 387001, IN
1 Pharmacognosy and Phytochemistry Department, Ramanbhai Patel College of Pharmacy, Charusat, At and Post – Changa, Dist. Anand, Gujarat, 388421, IN
2 J.S. Ayurveda College, College Road, Nadiad, Gujarat, 387001, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2383-2390Abstract
Ashwagandhadi lehya is traditionally used as aphrodisiac and spermatogenic. It is official in Ayurvedic Formulary of India. Root powder of Withania sominfera is incorporated as one of major ingredients in the formulation. The studies were aimed to evaluate Ashwagandhadi lehya, Clincially, as aphrodisiac and in treatment of Oligospermia. The duration of studies was 90 days and patients showed willingness to participate in studies were asked to submit blood and serum samples on 0, 45th and 90th day. Alteration in serum FSH, serum LH, serum testosterone as well as alteration in sperm concentration, % of normal sperms and % motile sperms in semen samples were selected as evaluation parameters to assess role of Ashwagandhadi lehya in treatment of oligospermia. Aphrodisiac potential of the formulation was evaluated by recording the responses of patients in a questioner, prepared on the basis of International Index of Erectile Function (IIEF- 5), included their experience in sexual desire, erection, penile rigidity, ejaculation and orgasm before and after treatment. The results showed that mean serum concentration of testosterone was increased by 8.31 % after 45 days of treatment and 16.53 % after 90 days of treatment, without concurrent alteration in serum FSH and serum LH concentration. Semen analysis showed that, after 90 days of treatment, mean sperm concentration in semen was increased by 200%, while % of normal sperms and % motile sperms were found to be increased by 11% and 59 %, respectively. The results of the studies showed, statistically significant improvement in sexual arousal, penile rigidity, orgasm and ejaculation, after treatment. As, testosterone concentration was increased in absence of increased LH release, it was concluded that formulation might act directly on testis and stimulated the expression of the proteins responsible for testosterone synthesis. It was also proposed that, increased in serum testosterone level might be responsible for aphrodisiac potential of Ashwagndhadi Lehya.Keywords
Ashwagandhadi lehya, Vajikaran, Oligospermia, Spermatogenic, Aphrodisiac, Withania somifera.
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- Assessment of Testosterone Biogenesis Enhancement by a Lupeol Derivative Separated from Seeds of Hygrophila spinosa T. Ander in vitro
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Authors
Affiliations
1 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
2 Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
1 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
2 Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
Source
Journal of Natural Remedies, Vol 21, No 4 (2021), Pagination: 317-322Abstract
The present study aimed towards assessing the isolated lupeol derivative in vitro for testosterone biogenesis enhancement by the action on isolated rat leydig cells. Studies were carried out to separate a novel bioactive molecule from an Indian medicinal plant, Hygrophila spinosa, used as an aphrodisiac and spermatogenic for years. Unsaponifiable bioactive fraction, prepared from seeds of the plant, was subjected to isolation of phytoconstituents by column and flash chromatography. The isolated compound was characterized by spectral studies as lupeol methyl ether. Later, it was subjected to in vitro studies using isolated leydig cells from rat for its action. Different amounts of isolated compound were incubated with leydig cells for 3 hours and concentration of testosterone after incubation was considered as a parameter. Dehydroepiandrosterone was used as positive control in the studies. The results indicated that the isolated compound was active and enhanced the testosterone biogenesis, as evident from increased amount of testosterone found in a medium treated with compound. This was the first report included isolation, chemical characterization and biological activity of Lupeol methyl ether from Hygrophila spinosa T. Ander.Keywords
Hygrophila spinosa, Lupeol, Leydig Cells, Steroids, Tri-terpenoids, Testosterone.Full Text
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