Informatics Publishing Limited

Hagga M. A. Mohamed ¹, Mohd. Imran ¹, Monadil H. M. Ali ¹, Mohammed F. Abdelwahab ¹, Abdullrahman A. Alhaj ²

Affiliations
1 Northern Border University, Rafha, 91911, PO Box 840, SA
2 KCMS, Khartoum, SD

Abstract

In the present study, a simple, inexpensive, precise and accurate uv-spectrophotometric method based on chemometrics, has been developed for the simultaneous determination of sulfadoxine and pyrimethamine in tablet formulation. The % recoveries obtained were 99.7% ± 0.9 and 101.5% ± 0.8 for sulfadoxine and pyrimethamine, respectively. The developed method has been compared to USP-HPLC method with regard to accuracy and precision. The calculated F-ratio and the (t) statistics indicate that there is no significant difference at 5% level with regard to precision and accuracy between the proposed and the USP methods. Moreover, the developed method is simple, cost-effective, and less time-consuming. Accordingly, it can be used advantageously in routine quality control of sulfadoxine and pyrimethamine in tablet formulation.

Keywords

Chemometrics, Pyrimethamine, Simultaneous Determination, Sulfadoxine, Tablets, UV.

Full Text

   

Vijay R. Chidrawar ¹, Mohd. Imran ²

Affiliations
1 Department of Pharmacology and Toxicology, Northern Border University, Rafha, SA
2 Department of Pharmaceutical Chemistry, Northern Border University, Rafha, SA

Abstract

Angiogenesis is the development of new blood vessels from pre-existing one. The ion-channels on endothelium plays vital role in cell proliferation and related angiogenesis. We aimed to investigate the effects of L-type (Verapamil and Diltiazem) and T-type (Ethosuximide) Calcium Channel Blockers (CCBs) on neovascularization. The effects on neovascularization were investigated by in-ovo (CAM, Chick Chorioallantoic membrane) and in-vitro (aortic ring assay) methods. Each test drug was tested for at least 3 doses and the anti-angiogenic effect was compared with Suramin as standard and normal control groups. Various vital parameters were recorded during the experiment like the number of blood capillaries, sprouts formation, angiogenic score etc. The L-type Ca²⁺ channel blockers Verapamil at the dose of 50μM, 110 and 220 μM/disk has shown significant (p < 0.001) reduction in the number of branching points in CAM assay. For the further confirmation, angiogenic activity was evaluated in vitro by rat aortic ring assay method; the area of sprouts was reduced by the medium and high dose of verapamil. Diltiazem has demonstrated modest anti-angiogenic activity by both the models, whereas T-type calcium channel blocker ethosuximide has not shown any effect on the neovascularization. Among all the tested drugs verapamil has shown the promising anti-angiogenic property. Thus verapamil and diltiazem may have antiangiogenic activity defines novel beneficial effects in angiogenic mediated pathological conditions in addition to their main indications in cardiovascular complications.

Keywords

Angiogenesis, CAM Assay, L-Type Ca²⁺ Channel Blockers, Rat Aortic Ring Assay, T-Type Calcium Channel Blocker.

Full Text