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Bag, Niladri
- Seed Germination Studies of Dev-ringal Thamnocalamus spathiflorus (TRIN.) Munro a Temperate Bamboo
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Indian Forester, Vol 139, No 7 (2013), Pagination: 610-614Abstract
Thamnocalamus spathiflorus (Trin.) Munro an evergreen, temperate bamboo is important in the Himalayan context. In this study seed storage, effect of temperature, GA3 and seven different type of lights were used for enhancing/uniform seed germination of this bamboo species. Sun- dried seeds were stored at room temperature (20-25°C) for four years and viability was 90-93% for the entire period. 25°C was found to be the best temperature in which after 3 days of sowing seed germination was started and 93.3% germination was recorded 12 days after sowing. Treatment of GA3 (250-5000 μM) was significant under the limitation of this experiment. Among the seven types of light treatments yellow light treatment gave the best performance with 43.3% germination at day three, 73.3% at day 5 and 100% by day 10. Red light was found to be the least effective and maximum 76.7% seeds were germinated with this treatment.Keywords
Thamnocalamus spathiflorus, Temperate Bamboo, Seed Germination, Light Treatment
- An Efficient Method for Acclimatization:in vitro Hardening of Tissue Culture-Raised Tea Plants (Camellia sinensis (l.) O. Kuntze)
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Affiliations
1 G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263 643, IN
2 Present address: Graphic Era Deemed to be University, Clement Town, Dehradun 248 002, IN
1 G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263 643, IN
2 Present address: Graphic Era Deemed to be University, Clement Town, Dehradun 248 002, IN
Source
Current Science, Vol 117, No 2 (2019), Pagination: 288-293Abstract
In the 21st century while there is all-round development in every sphere of science and technology, a major limitation in micropropagation technology still remains; a large number of plantlets of several species die during hardening and ex vitro transfer. In the present study, introducing an in vitro incubation period in plant growth regulator (PGR)-free medium followed by ischolar_main induction of micropropagated tea shoots (Camellia sinensis (L.) O. Kuntze) and increasing it to 12 weeks instead of the usual 4 weeks gave excellent results. Secondly, cultures were grown in larger vessels, i.e. in 500 ml conical flasks in place of normal 250 ml conical flasks. Interestingly, prolonged incubation (12 weeks) significantly enhanced overall growth, i.e. length, stem girth, leaf number and leaf area of plantlets. The use of larger-sized culture flask also positively influenced growth. The abovementioned two simple modifications in culture conditions improved overall growth of plantlets. Also, plantlets raised through prolonged culture were acclimatized with 94% survival efficiency in comparison to 46% with normal culture period, and showed improved growth performance on transfer to soil in the nursery, irrespective of the culture vessel size.Keywords
Acclimatization, in vitro Hardening, Lab-to-Land Transfer, Prolonged Culture, Tea Micropropagation.Full Text
References
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