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Vyas, Niraj
- Development and Validation of Stability Indicating HPTLC Method for Estimation of Ursolic Acid
Authors
Source
Asian Journal of Research in Chemistry, Vol 6, No 6 (2013), Pagination: 546-551Abstract
A stability indicating high performance thin layer chromatographic (HPTLC) method was developed and validated for estimation of ursolic acid. Study was performed on pre-coated silica gel TLC plates using toluene: ethyl acetate: formic acid (7:3:0.1, v/v/v) as the mobile phase. After development, plates were derivatized with Anisaldehyde- Sulphuric acid reagent. Detection and quantification were performed by densitometric scanning at 530 nm. Method was validated according to ICH Q2(R<SUB>1</SUB>)guideline. The linear regression analysis for the calibration plot showed good linear relationship over the concentration range of 200-600 ng/spot. The correlation coefficient of calibration curve was found to be 0.9986. The intra and inter day precision was found to be less than 2%. Ursolic acid was also subjected to acid, base, oxidation, heat, humidity and photo-degradation studies. The degradation products obtained were well resolved from the pure drug with significantly different Rf values. As the method could effectively separate the drugs from its degradation products, it can be used for stability-indicating analysis.Keywords
Ursolic Acid, Stability Indicating Method, High Performance Thin Layer Chromatography (HPTLC), Method ValidationReferences
- http://www.85xianji.com/xiangxi.asp?id=170 (assessed on 09/02/2013)
- http://suite101.com/article/ursolic-acid-a92128#.UWLDtJP-G3s (assessed 10/02/2013)
- Amin P, Nayak B and Patel K. Extraction and estimation of different marker compounds by HPTLC from Alstonia scholaris leaves and flowers. International Journal of Pharmaceutical Science. 2012: 2731-2738.
- Shetty PG, et al. Pharmacokinetic analysis of ursolic acid in Alstonia scholaris R. Br. by high-performance thin-layer chromatography. Journal of Planer Chromatography. 20(2); 2007: 117-120.
- Rout K, et al. Thin-layer chromatographic separation and validated HPTLC method for quantification of ursolic acid in various Ocimum Species. Journal of Food and Drug Analysis. 20(4); 2012: 865-871.
- Gupta M, et al. Determination of ursolic acid a biomarker in different Swertia species through high performance thin layer chromatography. Chinese Medicine. 2; 2011: 121-124.
- Mucaji P and Nagy M. Contribution to the TLC separation of ursolic and oleanolic acid mixture. Acta Facultatis Pharmceuticae Universitatis Comenianae. 58; 2011: 56 - 61.
- Almeida M, et al. Determination of ursolic acid from polymeric nanoparticles using high-performance liquid chromatography method. Latin American Journal of Pharmacy. 31(8); 2012: 1217-1221.
- Liu Ch and Liu Y. Determination of ursolic acid in herbs of Verbena officinalis by HPLC. Zhongguo Zhong Yao Za Zhi. 27(12); 2002: 916-918.
- Taralkar SV and Chattopadhyay S. A HPLC method for determination of ursolic acid and betulinic acids from their methanolic extracts of Vitex Negundo Linn. Journal of Analytical and Bioanalytical Techniques. 3(3); 2012: 134.
- Maurya A and Srivastava S. Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC. Journal of Brazilian Chemical Society. 23(3); 2012: 468-472.
- http://en.cnki.com.cn/Article_en/CJFDTOTALSZGY200711007. htm (assessed on 14/02/2013)
- Zacchigna M, et al. Rapid HPLC analysis for quantitative determination of the two isomeric triterpenic acids, oleanolic acid and ursolic acid, in Plantago Major. Scientia Pharmaceutica. 77; 2009: 79-86.
- Chun-Hua Zhou, et al. Oleanolic and ursolic acid in the fruit of Eriobotrya japonica Lindl. Journal of Medicinal Plants Research. 5(9); 2011: 1735-1740.
- Sundaram RS, et al. Quantification of bioactive principles in Indian traditional herb Ocimum Sanctum Linn. (Holy Basil) Leaves by high performance liquid chromatography. Asian Journal of Biomedical and Pharmaceutical Sciences. 1(3); 2011: 35-41.
- Srivastava P and Chaturvedi R. Simultaneous determination and quantification of three pentacyclic triterpenoids—betulinic acid, oleanolic acid, and ursolic acid—in cell cultures of Lantana camara L. In Vitro Cellular and Developmental Biology Plant. 46; 2010: 549–557.
- Xia Y, et al. Quantitation of ursolic acid in human plasma by ultra performance liquid chromatography tandem mass spectrometry and its pharmacokinetic study. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 879(2); 2011: 219-224.
- Qiongfeng Liao, et al. LC-MS determination and pharmacokinetic studies of ursolic acid in rat plasma after administration of the traditional chinese medicinal preparation Lu-Ying extract. Yakugaku Zassi. 125(6); 2005: 509-515.
- Sundaram RS,et al. LC-MS quantification of rosmarinic acid and ursolic acid in the Ocimum Sanctum Linn. leaf extract (Holy Basil, Tulsi), Journal of Liquid Chromatography & Related Technologies. 35(5); 2012: 634-650.
- Zhi-Rong Tan, et al. LC–MS–MS quantitative determination of ursolic acid in human plasma and its application to pharmacokinetic studies. Chromatographia. 72; 2010: 1107- 1113.
- Lacikova L, et al. A rapid tandem mass spectrometric assay for determination of ursolic acid –application to analysis of ursolic acid in four species of Staphylea L. and leaves of Staphylea Pinnata L. gathered during ontogenesis. Chromatographia. 63; 2006: 117-122.
- Janicsak G,et al. Gas Chromatographic method for routine determination of oleanolic and ursolic acids in medicinal plants. Chromatographia. 58; 2003: 295-299.
- Razborsek MA, et al. Determination of oleanolic, betulinic and ursolic acid in lamiaceae and mass spectral fragmentation of their trimethylsilylated derivatives. Chromatographia. 67; 2008: 433- 440.
- ICH Harmonized Tripartite Guideline Q2 (R1): Validation Of Analytical Procedure: Text and Methodology. London; 2005.
- Bakshi M and Singh S. Development of validated stability indicating assay-methods – critical review. Journal of Pharmaceutical and Biomedical Analysis. 28; 2002:1011- 1040.
- Development and Validation of RP-HPLC Method for Simultaneous Estimation of Nebivolol and Indapamide in Pharmaceutical Dosage Form
Authors
1 Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, At & Post- Changa, Ta- Petlad, Dist. - Anand, 388421, Gujarat, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 4, No 3 (2014), Pagination: 98-102Abstract
A simple, sensitive, precise and specific Reverse Phase High Performance Liquid Chromatographic method was developed and validated for the determination of Nebivolol and Indapamide in bulk and tablet dosage form. It was found that the excipient in the tablet dosage form does not interfere in the quantification of active drug by proposed method. The HPLC separation was carried out by reverse phase chromatography by Inertsil ODS C-18 (150 x 4.6 mm) column with a mobile phase composed of Buffer: Acetonitrile (60:40) in isocratic mode at a flow rate of 1 ml/min. The detection was monitored at 282 nm. The calibration curve for Nebivolol and Indapamide was linear from 25-225 μg/ml and 7.5-67.5 μg/ml respectively. The inter-day and intra-day precision was found to be within limits. The proposed method has adequate sensitivity, reproducibility and specificity for the determination of Nebivolol and Indapamide.Keywords
HPLC, Nebivolol, Indapamide, Validation, Tablet.- Assessment of Testosterone Biogenesis Enhancement by a Lupeol Derivative Separated from Seeds of Hygrophila spinosa T. Ander in vitro
Authors
1 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
2 Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
Source
Journal of Natural Remedies, Vol 21, No 4 (2021), Pagination: 317-322Abstract
The present study aimed towards assessing the isolated lupeol derivative in vitro for testosterone biogenesis enhancement by the action on isolated rat leydig cells. Studies were carried out to separate a novel bioactive molecule from an Indian medicinal plant, Hygrophila spinosa, used as an aphrodisiac and spermatogenic for years. Unsaponifiable bioactive fraction, prepared from seeds of the plant, was subjected to isolation of phytoconstituents by column and flash chromatography. The isolated compound was characterized by spectral studies as lupeol methyl ether. Later, it was subjected to in vitro studies using isolated leydig cells from rat for its action. Different amounts of isolated compound were incubated with leydig cells for 3 hours and concentration of testosterone after incubation was considered as a parameter. Dehydroepiandrosterone was used as positive control in the studies. The results indicated that the isolated compound was active and enhanced the testosterone biogenesis, as evident from increased amount of testosterone found in a medium treated with compound. This was the first report included isolation, chemical characterization and biological activity of Lupeol methyl ether from Hygrophila spinosa T. Ander.Keywords
Hygrophila spinosa, Lupeol, Leydig Cells, Steroids, Tri-terpenoids, Testosterone.References
- Nadkarni KM. Indian Materia Medica, Vol. 713, Popular Prakashan, Bombay; 1976
- Bhavmishra BS, Nighantu B. Pandit Shri Brahma Sankar Mishra with the Vidyotani Hindi Commentatry. Chaukhambha Sanskrit Sansthan, Varanasi; 2005.
- Kirtikar KR, Basu BD. Indian Medicinal Plants. Vol. 2, International book distributors, Dehradun. 1991. 1182 p.
- Basu NK, Rakhit S. Investigation of the seeds of Asteracantha longifolia Nees. Indian J Pharm. 1957; 19:282–4.
- Chauhan NS, Sharma V, Dixit VK. Effect of Asteracanthalongifolia seeds on the sexual behaviour of male rats. Nat Prod Res. 2011; 25(15):1423–31. https://doi.org/10.1080/14786410802588493
- Vyas NY, Raval MA. Aphrodisiac and spermatogenic potential of alkaloidal fraction of Hygrophila spinosa T. Ander in rats. J Ethnopharmacol. 2016; 194:947– 53. https://doi.org/10.1016/j.jep.2016.10.080
- Vyas NY, Raval M. Effect of unsaponifiable fraction of seeds of Hygrophila spinosa T. Ander on testosterone production of rat leydig cells in vitro. Asian J Pharm Clin Res. 2016; 9(6):184–6. https://doi.org/10.22159/ajpcr.2016.v9i6.14049
- MdYaseen K, Vimal K, Anuradha G, Niraj V, Siddharth P, Amee B. Reverse pharmacognosy in new drug discovery. Curr Pharma Res J. 2007; 1(5):31–6.
- Vyas N, Khan M, Panchal S, Butani A, Kumar V. Supercritical fluid technology: An unlimited frontier in herbal research. Int J Pharm Sci Nanotechnol. 2009; 1(4):303–8. https://doi.org/10.37285/ijpsn.2008.1.4.10
- Vyas N, Gamit K, Raval M, Patel S. Isolation and chemical characterization of bioactive alkaloid from Argyreia speciosa Linn. having action on isolated rat leydig cells. Asian J Pharm Clin Res. 2019; 12(10):276–80. https://doi.org/10.22159/ajpcr.2019. v12i10.34789
- Muhtadi FJ. Analytical profiles of drug substances and excipients. Vol. 23, Academic Press. Elsevier; 1994. p. 153–228. https://doi.org/10.1016/S0099-5428(08)60603-6
- Silverstein RM, Bassler GC, Morrill TC. Spectroscopy of organic compounds, New York, Chichester, Brisbane. Toronto, Singapore: John Wiley & Sons Inc; 2009.
- Urolithiasis: HPTLC Method for Quantitative Detection of Rutin and Quercetin in an Herbal Plant
Authors
1 Department of Pharmacology, Indukaka Ipcowala College of Pharmacy, New Vallabh Vidyanagar, Anand - 388121, Gujarat, IN
2 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Changa, Anand - 388421, Gujarat, IN
3 Department of Pharmacology, L.M. College of Pharmacy, Ahmedabad - 380058, Gujarat, IN
Source
Journal of Natural Remedies, Vol 22, No 3 (2022), Pagination: 371 - 379Abstract
Anti-inflammatory, Hypoglycemic, Hepatoprotective, Antihyperlipidemic, Anti-Ulcerative, Cardioprotective Stimulant, Sedative, Hypnotic, Anticonvulsant Activity, Memory Retention, Stroke Preventive Activity, Antimicrobial Activity, Antimycobacterial, Antiviral, Larvicidal, Antiparasitic, Chemopreventative, Chemo-modulatory, Anticancer, Cytoprotective activities were found in a variety of herbal plants, including Ocimum basilicum. The purpose of this study was to design and develop a new HPTLC method that was accurate, precise and cost-effective for simultaneous measurement of rutin and quercetin in a hydroalcoholic extract of Ocimum basilicum seeds. The mobile phase was Toluene: Ethyl Acetate: Methanol: formic acid (6:4:3:1, v/v/v/v/v) and densitometric scanning was performed at 254 nm. Merck TLC aluminium sheets of silica gel 60 F254, (10 x 10 cm) with a thickness of 250 mm was used as stationary phase. At a wavelength of 254 nm, rutin and quercetin were detected. The constituents were resolved satisfactorily, with Rf values of 0.25 ± 2.01 for rutin and 0.80 ± 0.64 for quercetin, respectively. Linearity (300-1300 ng/spot for rutin and quercetin) was used to test the method’s accuracy and reproducibility. For both analytes, intra- and inter-day precision, as evaluated by coefficient of variation, was less than 3%. For rutin, the detection and quantification limits were 46.52 and 140.96 ng/spot, respectively, and for quercetin, they were 81.79 and 247.84 ng/spot. The proposed approach was found to be precise, accurate, repeatable, and specific, and it could be used to measure quercetin and rutin in samples at the same time.
Keywords
Densitometry, Ocimum basilicum, Quercetin, High-performance Thin-layer Chromatography (HPTLC), Rutin, Validation.References
- Schreiner M, Huyskens-Keil S. Phytochemicals in fruit and vegetables: Health promotion and postharvest elici- tors. Cri Rev Plant Sci. 2006; 25:267-78. https://doi. org/10.1080/07352680600671661
- Soleas GJ, Grass L, Josephy PD, Goldberg DM, Diamandis EP. A comparison of the anticarcinogenic properties of four red wine polyphenols. Clin Biochem. 2002; 35:119-24. https:// doi.org/10.1016/S0009-9120(02)00275-8
- Middleton EM, Teramura AH. The role of flavonol gly- cosides and carotenoids in protecting soybean from ultraviolet-B damage. Plant Physiol. 1993; 103:741-52. https://doi.org/10.1104/pp.103.3.741. PMid:12231976. PMCid:PMC159044
- Hein KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants:
- Chemistry, metabolism and structure-activity relation- ships. J Nutr Biochem. 2002; 13(10):572-84. https://doi. org/10.1016/S0955-2863(02)00208-5
- Bors W, Heller W, Michel C, Saran M. Flavonoids as antioxidants: determination of radical-scavenging effi- ciencies. Method Enzymol. 1990; 186:343-55. https://doi. org/10.1016/0076-6879(90)86128-I
- Dixon RA, Dey PM, Lamb CJ. Phytoalexins: enzymology and molecular biology. Adv Enzymol RAMB.1983; 55:1-136.
- Rice-Evans CA, Miller NJ, Bolwell PG, Broamley PM, Pridham JB. The relative antioxidant activities of plant- derived polyphenolic flavonoids. Free Radical Res. 1995; 22(4):375-83. https://doi.org/10.3109/10715769509145649. PMid:7633567
- Agati G, Azzarello E, Pollastri S, Tattini M. Flavonoids as antioxidants in plants: location and functional signifi- cance. Plant Sci. 2012; 196:67-76. https://doi.org/10.1016/j. plantsci.2012.07.014. PMid:23017900
- Du F, Zhang F, Chen F, Wang A. Advances in microbial heter- ologous production of flavonoids. Afr J Microbiology Res. 2011; 5(18):2566-74. https://doi.org/10.5897/AJMR11.394
- Middleton EJ. Effect of plant flavonoids on immune and inflammatory cell function. Adv Exp Med Biol. 1998; 439:175-82. https://doi.org/10.1007/978-1-4615-5335- 9_13. PMid:9781303
- Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. Scientific World J. 2013; 2013:1- 16. https://doi.org/10.1155/2013/162750. PMid:24327805. PMCid:PMC3845396
- Nijveldt RJ, Nood EL, Van Horn DEC, Boelens PG, Van Norren K, van Leeuwen PAM. Flavonoids: A review of prob- able mechanisms of action and potential applications. Am J Clin Nutr. 2001; 74(4):418-25. https://doi.org/10.1093/ ajcn/74.4.418. PMid:11566638
- Bilal A, Jahan N, Ahmed A, Bilal SN, Habib S, Hajra S. Phytochemical and pharmacological studies on Ocimum basilicum linn — A review. Int J Curr Res. 2012; 4(23).
- International Conference on Harmonization. Validation of Analytical Procedures Methodology Q2B; 1996.
- Method for determination of forskolin in crude drug and pharmaceutical dosage form. Chromatographia. 2008; 67:441-47. https://doi.org/10.1365/s10337-008-0521-x
- Alam P, Ali M, Ahmad SA. A validated HPLC method for estimation of cordifolioside A in Tinospora cardifolia, Miers and marketed formulations. J Chromatogr Sci. 2009; 47: 910-13. https://doi.org/10.1093/chromsci/47.10.910. PMid:19930804
- Parveen R, Baboota S, Ahmad S, Ali J, Ahuja A. Stability- indicating HPTLC method for quantitative estimation of silybin in bulk drug and pharmaceutical dosage form. Biomed Chromatogr. 2010; 24:639-47. https://doi. org/10.1002/bmc.1340. PMid:19816854
- Rozylo JK, Janicka M. Different planar techniques for predic- tion of solute retention in column liquid chromatography. J Planar Chromatogr. 1996; 9:418-24.
- Weins C, Hauck HE. Advances and developments in thin layer chromatography. LC-GC Int. 1996; 4:455-71.
- Poole CF, Poole SK. Instrumental thin layer chromatography. Anal Chem. 1994; 66:27A-37A. https://doi.org/10.1021/ ac00073a001
- Sejal P, Niraj V. Validated spectrofluorimetric method for estimation of piperine in an ayurvedic formulation. Asian J Pharm Clin Res. 2012; 5(4):231-233.
- Niraj V, Sangita P. Development and Validation of RP-HPLC Method for Simultaneous estimation of Nebivolol and Indapamide in Pharmaceutical Dosage Form. Asian J Pharm Anal. 2014; 4(3):98-102.