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Somavanshi, Nisha
- Development and Validation of HPTLC Method for Simultaneous Estimation of Piperine and Scopoletin in Ajmodadi churna
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1 Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia - 391760, Vadodara, Gujarat, IN
1 Parul Institute of Pharmacy and Research, Parul University, Limda, Waghodia - 391760, Vadodara, Gujarat, IN
Source
Journal of Natural Remedies, Vol 23, No 1 (2023), Pagination: 143-148Abstract
In this recent study, several trials were made to develop a HPTLC method for quantification of scopoletin and piperine in the Ajmodadi formulation. HPTLC was done on pre-coated silica gel 60 F254 plates with a mobile phase of toluene:ethyl acetate:methanol:formic acid (3.9:3.9:0.3:1.7 v/v/v/v). The retardation factors for scopoletin 0.75 and for piperine was 0.86 and found a good and defined resolution peak. Densitometer analysis of scopoletin and piperine was carried out at 335 wavelength (nm). The developed method was validated as reported in ICH guidelines (Q2R1). A linearity study shows that scopoletin and piperine was linear, and the recovery studies revealed a recovery in between 98-102 % as per guideline. This method was established to be rapid, delicate, accurate and specific, thus, these methods may be used in the quantification of the drug in any polyherbal formulation.Keywords
Ajmodadi Churna, HPTLC, Piperine, Scopoletin.References
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