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Balan, P.
- RP-HPLC Method Development and Validation of Etodolac and Paracetamol in Combined Dosage Form
Authors
1 Dept. of Pharmaceutical Analysis, PRIST University, Thanjavur -614904, Tamilnadu, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 7 (2011), Pagination: 1073-1076Abstract
A simple, specific, accurate and precise reverse phase high pressure liquid chromatographic method has been developed for the simultaneous determination of Etodolac and Paracetamol from tablets. Stationary phase used was C18 column (symmetry C18, 250 mm x 4.5 mm). The sample was analyzed using acetonitrile: water in the ratio of 50:50 (pH adjusted to 5.8 with ortho phosphoric acid) as a mobile phase at a flow rate of 1.0 ml/min and detection was done at 232nm. The described method was linear over a concentration range of 2-14 μg/ml (r2 =0.999793 and0.999708) for Etodolac and Paracetamol respectively. The retention time for Etodolac and Paracetamol was found to be 1.932 and 2.456 min respectively, and recoveries from tablet were between 98.2%w/w and 101.2 %w/w. The limit of quantification (LOQ) for Etodolac and Paracetamol were found to be 0.15 and 0.04 μg/ml respectively The system suitability parameters were within the limit. The method developed was validated according to ICH guidelines which prove that the method was precise, accurate and can be used for routine determination of Etodolac and Paracetamol in bulk drug and its pharmaceutical dosage forms.
Keywords
Etodolac, Paracetamol, RP-HPLC Method, Validation.
- Simultaneous RP-HPLC Estimation of Ciprofloxacin Hydrochloride and Ornidazole in Tablet Dosage Form
Authors
1 Dept. of Pharmaceutical Analysis, Faculty of Pharmacy, PRIST University, Thanjavur -614904, Tamilnadu, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 2 (2011), Pagination: 227-230Abstract
A high performance liquid chromatographic method has been proposed for the simultaneous determination of ciprofloxacin hydrochloride and ornidazole in pure and dosage form. The HPLC instrument of Agilent technologies (Agilent 1120 LC Germany) model was used where the drug was chromatographed on a C-18 Zorabax column (4.6mm x 250mm) using a mixture of acetonitrile and water (Millipore Q, pH 3.0 adjusted with O-phosphoric acid) in the ratio of (45:55v/v) as the mobile phase at a flow rate of 1ml/min and the detection was done at 299nm (isobestic point). The retention time for ciprofloxacin HCL and ornidazole were 1.96 and 4.33 min respectively. The calibration curves were found to be linear in the range of 12 to 20 μg/ml with a correlation coefficient of 0.9998 and 0.9997 respectively. The results of analysis have been validated statistically and by recovery studies. The percentage recovery was obtained for ciprofloxacin HCL and ornidazole of 99.86 to 100.14% and 100.40 to100.53% respectively. The simplicity and accuracy of the proposed method ensures its use in routine quality control analysis of pharmaceutical formulations.