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Tiwari, Preeti

• Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Drakshasava Prepared by Traditional and Modern Methods

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Authors

Preeti Tiwari ¹, Rakesh K. Patel ²

Affiliations
1 Head of Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut (U.P.), IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN

Source

Asian Journal of Research in Chemistry, Vol 6, No 3 (2013), Pagination: 204-208

Abstract

The objective of the present study was to estimate the total phenolic content as well as flavonoids in Drakshasava-T and Drakshasava-M prepared by traditional and modern methods respectively and in its marketed preparation and also to evaluate the antioxidant activity of these test preparations on two different in vitro antioxidant activity models. Total phenolic content was determined colorimetrically using Folin-Ciocalteu reagent and was found 0.1045 and 0.1041 %w/w gallic acid equivalent in Drakshasava-T and Drakshasava-M respectively. Total flavonoid content was determined by aluminium chloride method and was found 0.01264 and 0.01214 %w/w quercetin equivalent in Drakshasava-T and Drakshasava-M respectively. Super-oxide anion scavenging activity and lipid per-oxidation assay were carried out to evaluate the antioxidant potential of Drakshasava-T and Drakshasava-M. The antioxidant activity of Drakshasava-T and Drakshasava-M was found increased in concentration dependent manner in both the in vitro antioxidant activity models as super-oxide radical scavenging activity and lipid per-oxidation assay. Drakshasava-T and Drakshasava-M showed significant scavenging of super-oxide radical and showed IC50 120.63 and 128.36 μg/ml respectively. Drakshasava-T and Drakshasava-M also inhibited the ferrous sulphate induced lipid per-oxidation in dose dependent manner and showed inhibitory concentration (IC50) 212.50 and 220.15 μg/ml respectively. Marketed Drakshasava also showed a rich concentration of total phenolics and flavonoids and showed dose dependent antioxidant activity in both the models. Thus, the results obtained in this study indicate that Drakshasava-T and Drakshasva-M can be a promising source of natural antioxidant.

Keywords

Total Phenolics, Flavonoids, Antioxidant Potential, Drakshasava

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References

• Richards RT and Sharma HM. Free radicals in health and disease. Industrial Journal of Clinical Practice 1991; 2(7): 15-26.
• Niwa Y. Effect of Maharishi four and Maharishi five on inflammatory mediators with special reference to their free radical scavenging effect. Industrial Journal of Clinical Practice 1991; 1(8): 23-27.
• Gutteridge JMC. Free radicals in disease processes: A compilation of cause and consequence. Free Radical Research Communication 1995; 19: 141.
• Ester S and Paolo S. Review on some plants of Indian traditional medicine with antioxidant activity. Journal of Ethnopharmacology 2000; 71: 23-43.
• Joyce DA. Oxygen radicals in disease. Advances in Drug Research Bulletin 1987; 127:476.
• Hertog MGL, Kromhout D and Aravanis C. Flavonoid intake and long term risk of coronary heart disease and cancer in seven countries study. Archives of Internal Medicine 1995; 155: 381- 386.
• The Ayurvedic Formulary of India, Part-II, 2000, 1st edition, The Controller of Publications, Delhi, p.35.
• Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15:335-339.
• Akoh CC, Bonilla EP, Sellappan S, Krewer G. Phenolic content and antioxidant capacity of Muscadine grapes. Journal of Agricultural and Food Chemistry 2003; 51:5497-5503.
• Frankel EN, Kanner J, German JB, Parks E, Kinsella JE. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. The Lancet 1993; 341(20):454- 457.
• Mayer AS, Yi OS, Person DA, Waterhouse DL, Frankel EN. Inhibition of human low density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vinifera). Journal of Agricultural and Food Chemistry 1997; 45:1638-1643.
• Teissedre PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Journal of the Science of Food and Agriculture 1996; 70:55-61.
• Waterhouse AL. Wine antioxidants may reduce heart disease and cancer. Presentation of American Chemical Society, Washington; 1994.
• Renaud S, Lorgeril MD. Wine, alcohol, platelets and the French paradox for coronary heart disease. The Lancet 1992; 339:1523- 1526.
• Davalos A, Bortolome B, Gomez-cordoves C. Antioxidant properties of commercial grape juices and vinegars. Food Chemistry 2005; 93(2):325-330.
• Orhan DD, Orhan N, Ergun E, Ergun F. Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats. Jornal of Ethnopharmacology 2007; 112:145-151.
• Corder R, Mullen W, Khan NQ, Marks SC, Wood EG, Carrier MJ, Crozier A. Red wine procyanidins and vascular health. Nature 2006;444:566.
• Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005.p. 253-254.
• Alam M, Radhamani S, Ali U, Purushottam KK. Microbiological Screening of Dhataki Flowers. Journal of Research in Ayurveda and Siddha 1984; 2(4):371-375.
• Singleton VL and Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 1965; 116:144-158.
• Kumar S. Antioxidant free radical scavenging potential of Citrullus colocynthis (L.) Schard. methanolic fruit extract. Acta Pharmaceutica 2008;58: 215-220.
• Nishimik M, Rao NA, Appaji N and Yagi K. The occurrence of superoxide anion in thereaction of reduced phenazine methosulphate and molecular oxygen. Biochemical and Biophysical Research Communication 1972; 46:849.
• Ohkawa H, Oshishi N and Yagi K. Assay for lipid peroxidation in animal tissues by thiobarbituricacid. Analytical Biochemistry 1979; 95:351.
• Lowery OH, Rosenbrough NJ, Farr AL and Randall RJ. Protein estimation with Folin phenol reagent. Biological Chemistry 1951; 193: 265.
• Cao G, Sofic E and Prior RL. Antioxidant capacity and prooxidant behaviour of flavonoids: structure activity relationships. Free Radical Biology and Medicine 1997; 22: 749-760.
• Wang H, Cao G and Prior RL. Oxygen radical absorbing capacity of anthocyanins. Journal of Agriculture and Food Chemistry 1997; 45: 304-309.
• Govindrajan R, Vjaykumar M, Rawat AKS and Mehrotra S. Free radical scavenging potential of Picrorrhiza kurroae. Indian Journal of Experimental Biology 2003; 3(41), 875.
• Purohit A and Vyas KB. Hypolipidemic efficacy of Capparis deciduas fruit and shoot extracts in cholesterol fed rabbits. Indian Journal of Experimental Biology 2005; 43:863-866.
• Bragghler JM, Duncan CA and Chase IR. The involvement of iron in lipid peroxidation . Importance of ferrous to ferric ion in initiation. Journal of Biological Chemistry 1986; 261:10282-89.

• Development and Validation of HPTLC Method for Quantification of Gallic acid and Catechin from Draksharishta

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Authors

Preeti Tiwari ¹, D.J. Sen ², Rakesh K. Patel ³

Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
3 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN

Source

Asian Journal of Research in Chemistry, Vol 6, No 3 (2013), Pagination: 248-253

Abstract

A simple, precise and accurate HPTLC method has been established for the determination of gallic acid and catechin in test formulations of Draksharishta as Draksharishta-T and Draksharishta-M prepared by traditional and modern method respectively and also in its marketed formulation. Draksharishta is a polyherbal hydro alcoholic preparation and is used to improve digestion, as blood purifier, in the treatment of anaemia and advised as a choice of remedy in respiratory problems. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ and specificity. The amount of gallic acid in Draksharishta-T, M and in its marketed formulation was found to be 0.0227, 0.0225 and 0.0226 % w/w respectively while catechin was found to be 0.0176, 0.0175 and 0.0175 %w/w respectively. Quantification of gallic acid and catechin in Draksharishta has been reported first time by validated HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and catechin from Draksharishta.

Keywords

Draksharishta, HPTLC, Validation, Gallic Acid, Catechin

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References

• The Ayurvedic Formulary of India Part-I, Controller of Publications, Delhi; 2000:15-16.
• Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15: 335-339.
• Frankel EN, Kanner J, German JB, parks E, Kinsella JE. Inhibition of oxidation of human low density lipoprotein by phenolic substances in red wine. Lancet 1993; 341 (20): 454-457.
• Mayer AS, Yi OS, Person DA, waterhouse DL, Frankel EN. Inhibition of human low density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vonofera). Journal of Agriculture and Food Chemistry 1997; 45: 1638-1643.
• Teissure PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Journal of Science Food and Agriculture 1996; 70:55-61.
• Waterhouse AL. Wine antioxidants may reduce heart disease and cancer. Presentation of American Chemical Society, Washington; 1994.
• Mishra S, Bhaisazya Kalpana Vigyan. Varanasi (India): Chaukambha Surbharati Prakashan; 2005:253-254.
• Alam M, Radhamani S, Ali U, Puroshottam KK. Microbiological screening of dhataki flowers. Journal of research in Ayurveda and Siddha 1984; 2(4): 371-375.
• ICH guidelines Q2 (R1). Validation of analytical procedures. Methodology, Geneva, 2006.
• Singh B, Mungara P, Nivsarkar M, Anandjiwala S. HPTLC densitometry quantification of glycyrrhizin, glycyrrhizinic acid, apigenin, kaempferol and quercetin from Glycyrrhiza glabra. Chromatographia 2009; 70: 1665-1672.
• R.P.W. Scott, Encyclopedia of Chromatography, 10th edn., Marcel Dekker, USA, 2001, p.p. 252-254.

• Evaluation of Antihyperlipidemic Potential of Drakshasava Prepared by Traditional and Modern Methods in Hyperlipidemic Rats

Abstract Views :650  |  PDF Views:2

Authors

Preeti Tiwari ¹, Rakesh K. Patel ²

Affiliations
1 Head of Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut, (U.P.), IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN

Source

Research Journal of Pharmacology and Pharmacodynamics, Vol 5, No 2 (2013), Pagination: 92-97

Abstract

The objective of the present study was to evaluate the lipid peroxidation activity and related antihyperlipidemic activity of Drakshasava-T and Drakshasava-M prepared by traditional and modern methods and its marketed formulation in high fat diet induced hyperlipidemic rats. The antioxidant activity of Drakshasava-T and Drakshasava-M was increased in concentration dependent manner. Drakshasava-T and Drakshasava-M inhibited the ferrous sulphate induced lipid peroxidation in a dose dependent manner and showed inhibitory concentration (IC50) value 212.50 and 220.15 μg/ml respectively. Oral administration of Drakshasava-T and Drakshasava- M for nine weeks at the dose of 2 ml/kg significantly reduced serum cholesterol, serum LDL and serum triglycerides while showed significant rise in serum HDL as compared to high fat diet fed control group. Marketed Drakshasava also showed significant decrease in serum cholesterol, serum LDL, serum triglycerides and showed significant rise in serum HDL. Atorvastatin (1.2 mg/kg, p.o.) was used as standard antihyperlipidemic drug. Both types of Drakshasava as Drakshasava-T and Drakshasava-M showed significant reduction in atherogenic index as compared to high fat diet fed control group which strongly supports antiatherosclerotic property of Drakshasava.

Keywords

Drakshasava, Lipid per Oxidation, Atherogenic Index, Antihyperlipidemic Activity, Atorvastatin

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References

• Tripathi. K.D .Essentials of Medical Pharmacology. 4th Edition, published by Jaypee Brothers, New Delhi, 2002; 612-614.
• Singh N, Kapur KK and Singh SP. Mechanism of cardiovascular action of Terminalia arjuna . J Med Plant Res. 1982; 45:102-104.
• The Ayurvedic Formulary of India, Part-II, 2000, 1st edition, The Controller of Publications, Delhi, p.35.
• Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15:335-339.
• Akoh CC, Bonilla EP, Sellappan S, Krewer G. Phenolic content and antioxidant capacity of Muscadine grapes. Journal of Agricultural and Food Chemistry 2003; 51:5497-5503.
• Frankel EN, Kanner J, German JB, Parks E, Kinsella JE. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. The Lancet 1993; 341(20):454- 457.
• Mayer AS, Yi OS, Person DA, Waterhouse DL, Frankel EN. Inhibition of human low density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vinifera). Journal of Agricultural and Food Chemistry 1997; 45:1638-1643.
• Teissedre PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Journal of the Science of Food and Agriculture 1996; 70:55-61.
• Waterhouse AL. Wine antioxidants may reduce heart disease and cancer. Presentation of American Chemical Society, Washington; 1994.
• Renaud S, Lorgeril MD. Wine, alcohol, platelets and the French paradox for coronary heart disease. The Lancet 1992; 339:1523- 1526.
• Davalos A, Bortolome B, Gomez-cordoves C. Antioxidant properties of commercial grape juices and vinegars. Food Chemistry 2005; 93(2):325-330.
• Orhan DD, Orhan N, Ergun E, Ergun F. Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats. Jornal of Ethnopharmacology 2007; 112:145-151.
• Corder R, Mullen W, Khan NQ, Marks SC, Wood EG, Carrier MJ, Crozier A. Red wine procyanidins and vascular health. Nature 2006;444:566.
• Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005.p. 253-254.
• Alam M, Radhamani S, Ali U, Purushottam KK. Microbiological Screening of Dhataki Flowers. Journal of Research in Ayurveda and Siddha 1984; 2(4):371-375.
• Braugghler JM, Duncan CA and Chase IR. The involvement of iron in lipid peroxidation. J Biol Chem. 1986; 261(22): 10282- 89.
• Lowery OH, Rosenbrough NJ, Farr AL and Randall RJ. Protein estimation with Folin phenol reagent. Biol Chem. 1951;193:265-275.
• Khanna AK, Chander R and Kapoor NK. Terminala arjuna: an Ayurvedic cardiotonic, Regulates lipid metabolism in Hyperlipidemic rats. Phytother Res. 1996;10: 663-665.
• Allain CC, Poon LS, Chan CS and Richmond W. Enzymatic Determination of Total Serum cholesterol. Clin Chem .1974;20::447-475.
• Friedewald WT, Levy RI and Fredrickson DS. Estimation of concentration of low density cholesterol in plasma without use of ultracentrifuge. J Clin Chem .1972;18: 449-502.
• Muller PH, Schmulling RM, Liebich HM and Eggstein M. A fully Enzymatic Triglyceride Determination. J Clin Chem. 1977;15:457-464.
• Anne SM, Ock SY, Debra AP, Andrew LW and Edwin NF. Inhibition of Human Low Density Lipoprotein oxidation in relation to composition of Phenolic antioxidants in Grapes (Vitis Vinifera). J Agri Food Chem. 1997;45 (5):1638-1643.
• S Renaud, and M De Lorgeril. Wine, alcohol, Platelets and the French Paradox for Coronary Heart Disease. The Lancet. 1992; 339: 1523-1526.
• Pederson TR. Low- density lipoprotein cholesterol lowering is and will be the key to the future of lipid management. American J Cardiol. 2001; 87(5A): 8B-12B.
• Boden WE and Pearson TA. Raising low levels of High Density Lipoprotein Cholesterol is an important target of therapy. American J cardiol. 2000; 85(5):645-650.

• Development and Validation of Simultaneous Equation UV-Spectrophotometric Method for the Estimation of Telmisartan and Amlodipine besylate in Combined Dosage Form

Abstract Views :310  |  PDF Views:1

Authors

Shishir Maheshwari ¹, Preeti Tiwari ², Atul Srivastav ¹

Affiliations
1 Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001
2 HOD, Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut

Source

Asian Journal of Research in Chemistry, Vol 6, No 8 (2013), Pagination: 765-768

Abstract

A simple, sensitive, precise and specific UV spectrophotometric method for simultaneous estimation of Telmisartan and Amlodipine besylate in combine dosage form has been developed. The wavelengths 238 nm and 295 nm were selected for estimation of Amlodipine besylate and Telmisartan respectively, where Methanol was used as solvent. Linearity were observed in the concentration range of 2-25 µg/ml (r2=0.9994) and 2-30 µg/ml (r2=0.9999) for Telmisartan and Amlodipine besylate respectively. These methods were validated according to ICH guidelines

Keywords

Telmisartan, Amlodipine Besylate, Simultaneous Equation Method

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References

• Gupta, Y., 2009. Isocratic RP-HPLC-UV method development and validation for the simultaneous estmation of ramipril and Telmisartan in tablet dosage form, Asian Journal of Pharmaceutical and Clinical Research, Vol. 2, Issue 4, pp. 104- 111.
• Tripathi, K.D., 2006. Essentials of medical pharmacology, Jaypee Brother’s Publishers, Edition 6, pp. 489.
• Taylor, J.B., and Triggle, D.J., 2006. Comprehensive Medicinal Chemistry, New York, Elsevier Sciences Inc, Vol. 2, pp. 10-15.
• Mahathi, G., 2011, development and validation of Telmisartan and Atorvastatin calcium in combined dosage form by RP-HPLC, International Journal Of Pharmacy and Technology, Vol. 3, Issue 3, pp. 3370-3389.
• Rajeswari, A., 2013. RP-HPLC method development and validation for Simultaneous estimation of Amlodipine besylate and Telmisartan in tablet dosage form, International Journal of Pharmaceutical Research and Analysis, Vol. 3, Issue 1, pp. 13-17.
• Cetin, G. and Sungur, S., 1995. A rapid and sensitive method for the spectrophotometric assay of amlodipine was based on the formation of a colored derived during the reaction of the drug, Scientia Pharmaceutica, Vol. 63, Issue 2, pp. 93-98.
• Mahant, S.S., Shetty, A.S., Gopinath, B., Ahmed, M. And Sridhar, B.K., 2010. A new reverse RP-HPLC method for the simultaneous determination of amlodipine besylate and Telmisartan in combined dosage form, International Journal of Chemical Sciences, Vol. 8, Issue 3, pp. 1895-1904.
• Moen, M.D., 2010. Worked on Telmisartan /amlodipine a singlepill combination of Telmisartan, an angiotensin II receptor antagonist, and amlodipine, a dihydropyridine calcium channel antagonist, WHO, Vol. 10, Issue 6, pp. 401-412.
• Marcia, L., 2003. Pediatric Pharmacotherapy, Jaypee brothers, Vol. 9 Issue 7, pp 86-95.
• Joel, G.H., et al., 1996. Goodman Gilman’s the pharmacological basis of therapeutics, New Jersy McGraw Hill Companies, Edition 9, Vol. 1, pp. 785-797.
• ICH, Q2 (R1) Validation of analytical procedure: Text and methodology, International Conference on Harmonization, Geneva, Switzerland, 2005.

• Development and Validation of Simultaneous Equation UV-Spectrophotometric Method for the Estimation of Metformin HCl and Glimepiride in Combined Dosage Form

Abstract Views :415  |  PDF Views:2

Authors

Atul Srivastav ¹, Preeti Tiwari ², Shishir Maheshwari ¹

Affiliations
1 Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001
2 HOD, Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut

Source

Asian Journal of Research in Chemistry, Vol 6, No 9 (2013), Pagination: 845-848

Abstract

A simple, sensitive, precise and specific UV spectrophotometric method for simultaneous estimation of Metformin HCl and Glimepiride in combined dosage form has been developed. Two wavelengths 233 nm and 228.4 nm were selected for estimation of Metformin HCl and Glimepiride respectively. Acetonitrile was used as solvent for analysis of both the drugs. Linearity was observed in the concentration range of 5-10 μg/ml (r2=0.9992) and 3-18 μg/ml (r2=0.9999) for Metformin HCl and Glimepiride respectively. The method was validated as per ICH guidelines.

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References

• Klepser T.B., Kelly M.W.; Metformin hydrochloride: An antihyperglycemic agent. Am J Health System Pharm. 1997; 54:893–903.
• Hitt E.;Uses of metformin may extend beyond patients with type 2 diabetes. Drugs. 1995; 63:1879–94.
• Maria C.R., Ranetti A.;The pharmacokinetics of some oral antidiabetics –A pharmaceutical approach. Farmacia, 2006; LIV, 6, 3- 13.
• Hermann L. S.; Metformin:A review of its pharmacological properties and therapeutic use. Diabete metab. 1979; 5(3): 233- 45.
• Budavari S, in. The Merck Index., ed. Merck and Co Inc., 13thed., 1997; Whitehouse Station (NJ), 5792.
• Arayne MS, Sultana N, Zuberi MH, Siddiqui FA. Spectrophotometric quantitation of Metformin in bulk drug and pharmaceutical formulations using multivariate technique. Indian J Pharm Sci 2009; 71 (3): 331-335.
• Chaturvedi PK, Sharma R. Simultaneous spectrophotometric estimation and validation of three component tablet formulation containing pioglitazone hydrochloride, Metformin hydrochloride and Glibenclamide. Analytical Letters 2008; 41(12):2133-2142.
• Rohokale BS, Jadhav VM, Kadam VJ. Studies in prospective process validation of Metformin HCl tablet Dosage formulation. Int J PharmaTech Res 2010; 2(3):1673-1678.
• Satoskar RG, Bhandarkar SD, Nirmala N, pharmacology and pharmacotherapeutics, Vol II,17th ed, Popular publication, Mumbai, Maharastra, India.
• Muller G, Hartz D, Punter J, Okonomopulos R, Kramer W; Differential interaction of glimepiride and glibenclamide with the beta-cell sulfonylurea receptor. I. Binding characteristics. Biochim Biophys Acta. 1994; 1191(2):267-77.
• ICH, Q2 (R1) Validation of analytical procedure: Text and methodology, International Conference on Harmonization, Geneva, Switzerland, 2005.

• Cardioprotective Activity of Amritarishta on Isoproterenol Induced Myocardial Infarction

Abstract Views :268  |  PDF Views:0

Authors

Preeti Tiwari ¹

Affiliations
1 Department of Pharmacognosy, Dr K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar (U. P.), IN

Source

Research Journal of Pharmacology and Pharmacodynamics, Vol 5, No 6 (2013), Pagination: 356-361

Abstract

The present study was designed to evaluate the cardio protective activity of Amritarishta-T, Amritarishta-M prepared by traditional and modern methods respectively and its marketed preparation on isoproterenol (ISO) induced myocardial infarction (MI) in albino rats. Wistar albino rats of either sex were randomly divided into 06 groups comprising 06 animals in each group as normal control, ISO control, pretreatment with Inderal*10 (10 mg/kg) per os, pretreatment with Amritarishta-T, M and its marketed preparation at the dose of 2 ml/kg per os per day for 30 days. MI was induced in all the groups except normal control, by administering ISO (85 mg/kg) intraperitoneally, on 29th and 30th day. On 31st day, level of serum marker enzymes was determined and serum lipid profile was also measured. Then, animals were subsequently sacrificed, hearts were removed, weighed and immediately processed for biochemical studies. Pretreatment with Inderal*10 and all the test preparations of Amritarishta significantly prevented the ISO-induced adverse changes in the levels of serum marker enzymes as creatine kinase (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and also improved serum lipid profile. All the test formulations pretreated groups showed significant increase in glutathione (GSH) content and significantly reduced malonyldialdehyde (MDA). Thus, experimental finding suggests that the cardio protective activity of Amritarishta-T, M and its marketed preparation may be due to an augmentation of endogenous antioxidants as GSH and inhibition of lipid peroxidation of cardiac membrane.

Keywords

Myocardial Infarction, Isoproterenol, Amritarishta.

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References

• Bolli R. Myocardial ischemic metabolic disorder leading to cell death. Rev Post Cardiol 1994; 13:649-53.
• Dhar ML, Dhar MM, Dhawan BN, Ray C. Screening of Indian plants for biological activity. J Exp Biol 1968; 6:232-47.
• Hertog MGL, Feskens EJM, Hollam PCH, Katan MB, Kromhout D. Dietary antioxidant flavonoids and risk of coronary heart diseases. Lancet 1993; 342:1007-20.
• The Ayurvedic Formulary of India, Part-I. 2000, 1^st edition, The Controller of Publications, Delhi, 6.
• Kumar S, Verma NS, Pande D and Srivastava PS. In vitro regeneration and screening of berberinein Tinospora cordifolia. Journal of Medicinal and Aromatic Plant Science 2000;22:61.
• Biset NG and Nwaiwu J.Quaternary alkaloids of Tinospora species. Planta Medica 1983;48:275-9.
• Maurya R, Wazir V, Tyagi A and Kapil RS. Cordifoliosides A and B, two new phenylpropene disaccharides from Tinospora cordifolia possessing immunostimulant activity. Natural Product Letter 1996;8:7-10.
• Gangan VD, Pradhan P, Sipahimalani AT and Banerji A. Cordifoliosides A, B, C:Norditerpene furan glycosides from Tinospora cordifolia. Phytochemistry 1994; 37:781-6.
• Dixit SN and Khosa RL. Chemical investigation of Tinospora cordifolia. Indian Journal of Applied Chemistry 1971;34:46-7.
• Maurya R and Handa SS. Tinocordifolin, a sesquiterpene from Tinospora cirdifolia. Phytochemistry 1998;49:1343-6.
• Kidwai AR, Salooja KC, Sharma VN, Siddiqui S. Chemical examination of Tinospora cordifolia. Journal of Science and Indian Research 1949; 8:115-8.
• Stanely M, Prince P and Menon VP. Antioxidant action of Tinospora cordifolia ischolar_main extract in alloxan diabetic rats.Phytotherapy Research 2001; 15:213-8.
• Mehrotra R, Katiyar CK and Gupta AP. Hepatoprotective compositions and composition for treatment of conditions related to hepatitis-B and E infection. US Patent 749296. 2000.
• Prince PS and Menon VP. Antioxidant activity of Tinospora cordifolia ischolar_mains in experimental diabetes. Journal of Ethnopharmacology 1999; 65:277-81.
• Ikram M, Khattak SG and Gilani SN. Antipyretic studieson some indigenous Pakistani medicinal plants. Journal of Ethnopharmacology 1987; 19:185-92.
• Manjrekar PN, Jolly CI and Narayanan S. Comparative studies of immunomodulatory activity of Tinospora cordifolia and Tinospora sinensis. Fitoterapia 2000; 71:254-7.
• Jagetia GC, Nayak V and Vidyasagar MS. Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in cultured HeLa cells. Cancer Letter 1998;127:71-82.
• Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005.p. 253-254.
• Alam M, Radhamani S, Ali U and Purushottam KK. Microbiological Screening of Dhataki flowers. Journal of Research in Ayurveda and Siddha 1984; 2(4):371-375.
• Rona G, Chapel CI, Balazs T, Gaudry R. An infarct like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat. Arch Pathol 1959;76:443-55.
• Tripathi KD. Essentials of Medical Pharmacology. 6th ed. New Delhi (India): Jaypee Brothers Medical Publishers Limited; 2008. p. 137-8, 537.
• Varley H. Practical Clinical Biochemistry. 4th ed. NY: William Heinemann; 1967.p. 161-2.
• Lamprecht W, Stan F, Weisser H, Heinz F. Determination of creatine phosphate and adenosine triphosphate with creatine kinase. In: Methods of Enzymatic analysis. Ed. HU Vergmeyer. NY: Academic Press; 1974. p. 1776-8.
• Mohun AF, Cook IGY. Simple methods for measuring serum levels of Glutamic oxaloacetic and Glutamic pyruvic transaminases in routine laboratories. J Clin Pathol 1957;10 (4):394-9.
• Allain CC, Pool LS, Chan CS, Richmond W. Enzymatic determination of serum cholesterol. Clin Chem 1974;20:447-75.
• Friedewald WT, Levy RI, Fredrickson DS. Estimation of the Concentration of Low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18 :499-502.
• Muller PH, Schmulling RM, Liebich HM, Eggstein M. A fully enzymatic triglyceride determination. J Clin Chem 1977;15:457-64.
• Ohkawa H, Ohisi N, Yagi K. Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
• Ellman GL. Tissue Sulphydril groups. Arch Biochem Biophys 1959;82:72-7.
• Nirmala C, Puvanakrishnan R. Isoproterenol induced myocardial infarction in rats; functional and biochemical alterations. Med Sci Res 1994;22:575-7.
• Haenen GR, Veerman M, Bast A. Reduction of beta adrenoceptor functions by oxidative stress in heart. Free Radic Biol Med 1990;9:279-88.
• Tappia PS, Heta T, Dhalla NS. Role of oxidative stress in catecholamine induced changes in cardiac sarcolemmal Ca2+ transport. Arch Biochem Biophys 2001; 377:85-92.
• Pederson TR. Low density lipoprotein cholesterol lowering is and will be the key to the future of lipid management. Am J cardiol 2001; 87(5A):8B-12B.
• Bolden WE, Pearson TA. Raising low levels of High density lipoprotein cholesterol is an important target of therapy. Am J cardiol 2000;85(5):645-50.

• Evaluation of Diuretic Potential of Amritarishta Prepared by Traditional and Modern Methods in Experimental Albino Rats

Abstract Views :629  |  PDF Views:0

Authors

Preeti Tiwari ¹

Affiliations
1 Department of Pharmacognosy, Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar (U. P.), IN

Source

Research Journal of Pharmacology and Pharmacodynamics, Vol 6, No 2 (2014), Pagination: 71-74

Abstract

The objective of the present study was to evaluate the diuretic potential of Amritarishta-T and Amritarishta-M prepared by traditional and modern methods respectively and its marketed formulation in experimental rats using furosemide (10 mg/kg p.o.) as a standard diuretic drug. Oral administration of Amritarishta-T, Amritarishta-M and its marketed formulation at the dose of 2.0 ml/kg over a period of 5 h showed a significant increase in urine volume as compared to control group. Both types of Amritarishta as Amritarishta-T and Amritarishta-M prepared by traditional and modern methods respectively and its marketed formulation showed significant increase in sodium, potassium and chloride level in urine sample as compared to control group. The maximum diuretic effect was produced by furosemide. Thus, both types of Amritarishta as Amritarishta-T and Amritarishta-M and its marketed formulation showed significant diuretic, natriuretic and kaliuretic effects.

Keywords

Diuretic Potential, Furosemide, Amritarishta, Natriuretic Effect, Kaliuretic Effect.

Full Text

     

References

• The Ayurvedic Formulary of India, Part-I. 2000, 1^st edition, The Controller of Publications, Delhi, 6.
• Kumar S, Verma NS, Pande D and Srivastava PS. In vitro regeneration and screening of berberinein Tinospora cordifolia. Journal of Medicinal and Aromatic Plant Science 2000;22:61.
• Biset NG and Nwaiwu J. Quaternary alkaloids of Tinospora species. Planta Medica 1983;48:275-9.
• Maurya R, Wazir V, Tyagi A and Kapil RS. Cordifoliosides A and B, two new phenylpropene disaccharides from Tinospora cordifolia possessing immunostimulant activity. Natural Product Letter 1996;8:7-10.
• Gangan VD, Pradhan P, Sipahimalani AT and Banerji A. Cordifoliosides A, B, C: Norditerpene furan glycosides from Tinospora cordifolia. Phytochemistry 1994; 37:781-6.
• Dixit SN and Khosa RL. Chemical investigation of Tinospora cordifolia. Indian Journal of Applied Chemistry 1971; 34:46-7.
• Maurya R and Handa SS. Tinocordifolin, a sesquiterpene from Tinospora cirdifolia. Phytochemistry 1998; 49:1343-6.
• Kidwai AR, Salooja KC, Sharma VN, Siddiqui S. Chemical examination of Tinospora cordifolia. Journal of Science and Indian Research 1949; 8:115-8.
• Stanely M, Prince P and Menon VP. Antioxidant action of Tinospora cordifolia ischolar_main extract in alloxan diabetic rats. Phytotherapy Research 2001; 15:213-8.
• Mehrotra R, Katiyar CK and Gupta AP. Hepatoprotective compositions and composition for treatment of conditions related to hepatitis-B and E infection. US Patent 749296. 2000.
• Prince PS and Menon VP. Antioxidant activity of Tinospora cordifolia ischolar_mains in experimental diabetes. Journal of Ethnopharmacology 1999; 65:277-81.
• Ikram M, Khattak SG and Gilani SN. Antipyretic studies on some indigenous Pakistani medicinal plants. Journal of Ethnopharmacology 1987; 19:185-92.
• Manjrekar PN, Jolly CI and Narayanan S. Comparative studies of immunomodulatory activity of Tinospora cordifolia and Tinospora sinensis. Fitoterapia 2000;71:254-7.
• Jagetia GC, Nayak V and Vidyasagar MS. Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in cultured HeLa cells. Cancer Letter 1998; 127:71-82.
• Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005.p. 253-254.
• Alam M, Radhamani S, Ali U and Purushottam KK. Microbiological Screening of Dhataki flowers. Journal of Research in Ayurveda and Siddha 1984; 2(4):371-375.
• Lipschitz WL, Hadidian Z, Kerpcsar A. Bioassay of Diuretics. Journal of Pharmacology and Experimental Therapeutics 1943; 79: 97-110.
• Afzal M, Khan NA, Ghufran A, Iqbal A, Inamuddin M. Diuretic and nephroprotective effect of Jawarish Zarooni Sada - a polyherbal Unani formulation. Journal of Ethnopharmacology 2004;91:219-223.
• Loew D, Heimsoth V, Erwin K, Schilcher H. 1991. Diureticos: Quimica, Farmacologiay Therapeutica incluida Fitoterapia, Barcelona, Salvat Editores S.A.:270.
• Das PK, Goswami S, Chinniah A. Woodfordia fruticosa: Traditional uses and recent findings. Journal of Ethnopharmacology 2007; 110:189-199.
• Hollman PCH, Katan MB. Dietary Flavonoids: Intake, Health effects and bioavailability. Food and Chemical Toxicology 1999; 37:937-942.

• Antidiabetic Potential of Amritarishta Prepared by Traditional and Modern Methods in Alloxan Induced Diabetic Rats

Abstract Views :319  |  PDF Views:1

Authors

Preeti Tiwari ¹

Affiliations
1 Department of Pharmacognosy, Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar, Uttar Pradesh, IN

Source

Research Journal of Pharmacology and Pharmacodynamics, Vol 6, No 3 (2014), Pagination: 129-134

Abstract

The objective of the present study was to evaluate the effect of Amritarishta-T and Amritarishta-M prepared by traditional and modern methods respectively and marketed Amritarishta on fasting blood glucose and serum lipid profile in alloxan induced diabetic rats. Oral administration of Amritarishta-T, Amritarishta-M and marketed Amritarishta ( 2 ml/kg p.o.) for 21 days caused a significant decrease in fasting blood glucose (FBG) and showed significant rise in blood glutathione level (GSH) in diabetic rats. Glibenclamide was used as a standard antidiabetic drug (10 mg/kg, p.o). These preparations also caused significant reduction in serum cholesterol, LDL and triglycerides and showed significant rise in serum HDL level in diabetic albino rats. Thus all these preparations were able to maintain the tested parameters near to the normal level significantly.

Keywords

Cardiovascular Risk, Blood Glucose, Anti-Diabetic, Glutathione, Lipid Profile, Amritarishta, Alloxan.

Full Text

     

References

• Mohanty P, Hamouda W, Garg R, Aljada A, Ghanim H , Dandona P. Glucose challenge stimulates reactive oxygen species (ROS) generation by leucocytes. J Clin Endocrinol Met 2000; 85:2970-2973.
• Pickup JC, William G. Epidemiology of Diabetes mellitus. In Textbook of Diabetes. vol.I, 2nd ed. Blackwell, Oxford; 1997. p. 3.1-3.28.
• Sagara M, Satoh J, Wada R, Yagihashi S, Takahashi K, Fukuzawa M, Muto G, MutoY , Toyota T. Inhibition of development of peripheral neuropathy in STZ-induced diabetic rats with N-acetylcysteine. Diabetologia 1996; 53:446-449.
• Kesari AN, Gupta RK, Watal G. Hypoglycemic effects of Murraya koenigii on normal and alloxan diabetic rabbits. J Ethnopharmacol 2005; 97: 247-251.
• Davidson MB. Diabetes mellitus Diagnosis and treatment. New York: Wiley; 1981. p. 27-48.
• The Ayurvedic Formulary of India, Part-I. 2000, 1^st edition, The Controller of Publications, Delhi, 6.
• Kumar S, Verma NS, Pande D and Srivastava PS. In vitro regeneration and screening of berberinein Tinospora cordifolia. Journal of Medicinal and Aromatic Plant Science 2000;22:61.
• Biset NG and Nwaiwu J.Quaternary alkaloids of Tinospora species. Planta Medica 1983;48:275-9.
• Maurya R, Wazir V, Tyagi A and Kapil RS. Cordifoliosides A and B, two new phenylpropene disaccharides from Tinospora cordifolia possessing immunostimulant activity. Natural Product Letter 1996;8:7-10.
• Gangan VD, Pradhan P, Sipahimalani AT and Banerji A. Cordifoliosides A, B,C:Norditerpene furan glycosides from Tinospora cordifolia. Phytochemistry 1994;37:781-6.
• Dixit SN and Khosa RL. Chemical investigation of Tinospora cordifolia. Indian Journal of Applied Chemistry 1971;34:46-7.
• Maurya R and Handa SS. Tinocordifolin, a sesquiterpene from Tinospora cirdifolia. Phytochemistry 1998;49:1343-6.
• Kidwai AR, Salooja KC, Sharma VN, Siddiqui S. Chemical examination of Tinospora cordifolia. Journal of Science and Indian Research 1949; 8:115-8.
• Stanely M, Prince P and Menon VP. Antioxidant action of Tinospora cordifolia ischolar_main extract in alloxan diabetic rats.Phytotherapy Research 2001;15:213-8.
• Mehrotra R, Katiyar CK and Gupta AP. Hepatoprotective compositions and composition for treatment of conditions related to hepatitis-B and E infection. US Patent 749296. 2000.
• Prince PS and Menon VP. Antioxidant activity of Tinospora cordifolia ischolar_mains in experimental diabetes. Journal of Ethnopharmacology 1999;65:277-81.
• Ikram M, Khattak SG and Gilani SN. Antipyretic studieson some indigenous Pakistani medicinal plants. Journal of Ethnopharmacology 1987;19:185-92.
• Manjrekar PN, Jolly CI and Narayanan S. Comparative studies of immunomodulatory activity of Tinospora cordifolia and Tinospora sinensis. Fitoterapia 2000;71:254-7.
• Jagetia GC, Nayak V and Vidyasagar MS. Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in cultured HeLa cells. Cancer Letter 1998;127:71-82.
• Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005.p. 253-254.
• Alam M, Radhamani S, Ali U and Purushottam KK. Microbiological Screening of Dhataki flowers. Journal of Research in Ayurveda and Siddha 1984; 2(4):371-375.
• Nishikant AR, Naresh JG. Antidiabetic Activity of hydroethanolic extract of Cyperus rotundus in alloxan induced Diabetes in rats. Fitoterapia 2006; 77:585-588.
• Sharma SR, Dwivedi SK, Swarup D. Hypoglycemic and hypolipidemic effects of Cinnamomum tamala Nees leaves. Indian J Exp Biol 1996; 34:372-374.
• Giordano BP, Thrash W, Hollenbaugh L, Dube WP, Hodges C, Swain A, Banion CR , Klingensmith GJ. Performance of seven blood glucose testing systems at high altitude. Diabet Educa 1989; 15: 444-448.
• Allain CC, Poon LS, Chan CS, Richmond W. Enzymatic Determination of Total Serum cholesterol. Clin Chem 1974; 20: 471-475.
• Muller PH, Schmulling RM, Liebich HM, Eggstein M. A fully Enzymatic Triglyceride Determination. J Clin Chem 1977; 15: 457-464.
• Friedewald WT, Levy RI, Fredrickson DS. Estimation of the Concentration of Low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18: 499-502.
• Bowers LD. Kinetic Serum Creatinine Assay. The role of various factors in determining specificity. Clin Chem 1980; 26: 551-554.
• Wilson BW. Automatic Estimation of urea using urease and alkaline phenol. Clin Chem 1966; 12: 360-368.
• Sasaki MA. New method for the determination of serum alkaline phosphatase. Use of Berthelot's reaction for the estimation of phenol released by enzymatic activity. Igaku To Seibutsugaku 1966; 70: 208-214.
• Beutler E, Duron O, Kelly BM. Improved method for determination of blood glutathione. J Lab Med 1963; 61:882-888.
• Proks P, Reimann F, Gribble F. Sulfonyl urea stimulation in insulin secretion. Diabetes 2002; 51: S368-76.
• Shankar PK, Kumar V, Rao N. Evaluation of Anti-diabetic activity of Ginkgo biloba in streptozotocin induced Diabetic Rats. Iranian J Pharmacol Therapeutics 2005; 4: 16-19.
• Wohaleb SA, Godin DV. Alterations in free radical tissue defence mechanism in streptozotocin induced diabetes in rat. Diabetes 1987; 36: 1014-1018.
• Mitra SK, Gopumadhvans, Muralidhar TS, Anturlikar SD, Sujatha MB. Effect of D-400, a mineraloherbal preparation on lipid profile, glycosylated haemoglobin and glucose tolerance in streptozotocin induced diabetic rats. Indian J Exp Biol 1995; 33: 798-800.
• Bopanna KN, Kanna J, Sushma G, Balaram R, Rathod SP. Antidiabetic and antihyperlipidemic effects of neem seed kernel powder on alloxan diabetic rabbits. Indian J Pharmacol 1997; 29:162-7.
• Cho SY, Park JY, Park EM. Alteration of hepatic antioxidant enzyme activities and lipid profile in streptozotocin induced diabetic rats by supplementation of dandelion water extract. Clin Chem Acta 2002; 317: 109-117.

• Evaluation of Diuretic Potential of Drakshasava Prepared by Traditional and Modern Methods in Experimental Albino Rats

Abstract Views :222  |  PDF Views:0

Authors

Preeti Tiwari ¹, Rakesh K. Patel ²

Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN

Source

Research Journal of Pharmacology and Pharmacodynamics, Vol 4, No 5 (2012), Pagination: 281-284

Abstract

The objective of the present study was to evaluate the diuretic potential of Drakshasava-T and Drakshasava-M prepared by traditional and modern methods respectively and its marketed formulation in experimental rats using furosemide (10 mg/kg p.o.) as a standard diuretic drug. Oral administration of Drakshasava-T, Drakshasava-M and its marketed formulation at the dose of 2.0 ml/kg over a period of 5 h showed a significant increase in urine volume as compared to control group. Both types of Drakshasava as Drakshasava-T and Drakshasava-M prepared by traditional and modern methods respectively and its marketed formulation showed significant increase in sodium, potassium and chloride level in urine sample as compared to control group. The maximum diuretic effect was produced by furosemide. Thus, both types of Drakshasava as Drakshasava-T and Drakshasava-M and its marketed formulation showed significant diuretic, natriuretic and kaliuretic effects.

Keywords

Diuretic Potential, Furosemide, Drakshasava, Natriuretic Effect, Kaliuretic Effect.

Full Text

     

References

• The Ayurvedic Formulary of India, Part-II, 2000, 1^st edition, The Controller of Publications, Delhi, p.35.
• Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15:335-339.
• Akoh CC, Bonilla EP, Sellappan S, Krewer G. Phenolic content and antioxidant capacity of Muscadine grapes. Journal of Agricultural and Food Chemistry 2003; 51:5497-5503.
• Frankel EN, Kanner J, German JB, Parks E, Kinsella JE. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. The Lancet 1993; 341(20):454- 457.
• Mayer AS, Yi OS, Person DA, Waterhouse DL, Frankel EN. Inhibition of human low density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vinifera). Journal of Agricultural and Food Chemistry 1997; 45:1638-1643.
• Teissedre PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Journal of the Science of Food and Agriculture 1996; 70:55-61.
• Waterhouse AL. Wine antioxidants may reduce heart disease and cancer. Presentation of American Chemical Society, Washington; 1994.
• Renaud S, Lorgeril MD. Wine, alcohol, platelets and the French paradox for coronary heart disease. The Lancet 1992; 339:1523- 1526.
• Davalos A, Bortolome B, Gomez-cordoves C. Antioxidant properties of commercial grape juices and vinegars. Food Chemistry 2005; 93(2):325-330.
• Orhan DD, Orhan N, Ergun E, Ergun F. Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats. Jornal of Ethnopharmacology 2007; 112:145-151.
• Corder R, Mullen W, Khan NQ, Marks SC, Wood EG, Carrier MJ, Crozier A. Red wine procyanidins and vascular health. Nature 2006;444:566.
• Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005.p. 253-254.
• Alam M, Radhamani S, Ali U, Purushottam KK. Microbiological Screening of Dhataki Flowers. Journal of Research in Ayurveda and Siddha 1984; 2(4):371-375.
• Lipschitz WL, Hadidian Z, Kerpcsar A. Bioassay of Diuretics. Journal of Pharmacology and Experimental Therapeutics 1943; 79:97-110.
• Afzal M, Khan NA, Ghufran A, Iqbal A, Inamuddin M. Diuretic and nephroprotective effect of Jawarish Zarooni Sadaa polyherbal Unani formulation. Journal of Ethnopharmacology 2004;91:219-223.
• Loew D, Heimsoth V, Erwin K, Schilcher H. 1991. Diureticos: Quimica, Farmacologiay Therapeutica incluida Fitoterapia, Barcelona, Salvat Editores S.A.:270.
• Das PK, Goswami S, Chinniah A. Woodfordia fruticosa: Traditional uses and recent findings. Journal of Ethnopharmacology 2007; 110:189-199.
• Hollman PCH, Katan MB. Dietary Flavonoids: Intake, Health effects and bioavailability. Food and Chemical Toxicology 1999; 37:937-942.

• Cardioprotective Activity of Ashwagandharishta on Isoproterenol Induced Myocardial Infarction

Abstract Views :238  |  PDF Views:0

Authors

Preeti Tiwari ¹, Rakesh K. Patel ²

Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN

Source

Research Journal of Pharmacology and Pharmacodynamics, Vol 4, No 5 (2012), Pagination: 294-298

Abstract

The present study was designed to evaluate the cardio protective activity of Ashwagandharishta-T, Ashwagandahrishta-M prepared by traditional and modern methods respectively and its marketed preparation on isoproterenol (ISO) induced myocardial infarction (MI) in albino rats. Wistar albino rats of either sex were randomly divided into 06 groups comprising 06 animals in each group as normal control, ISO control, pretreatment with Inderal*10 (10 mg/kg) per os, pretreatment with Ashwagandharishta-T, M and its marketed preparation at the dose of 2 ml/kg per os per day for 30 days. MI was induced in all the groups except normal control, by administering ISO (85 mg/kg) intraperitoneally, on 29th and 30th day. On 31st day, level of serum marker enzymes was determined and serum lipid profile was also measured. Then, animals were subsequently sacrificed, hearts were removed, weighed and immediately processed for biochemical studies. Pretreatment with Inderal*10 and all the test preparations of Ashwagandharishta significantly prevented the ISO-induced adverse changes in the level of serum marker enzymes as creatine kinase (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and also improved serum lipid profile. All the test formulations pretreated groups showed significant increase in glutathione (GSH) content and significantly reduced malonyldialdehyde (MDA). Thus, experimental finding suggests that the cardio protective activity of Ashwagandharishta-T, M and its marketed preparation may be due to an augmentation of endogenous antioxidants as GSH and inhibition of lipid peroxidation of cardiac membrane.

Keywords

Myocardial Infarction, Isoproterenol, Ashwagandharishta.

Full Text

     

References

• Bolli R. Myocardial ischemic metabolic disorder leading to cell death. Reviews of Postgraduate Cardiology 1994; 13:649-53.
• Dhar ML, Dhar MM, Dhawan BN, Ray C. Screening of Indian plants for biological activity. Journal of Experimental Biology 1968; 6:232-47.
• Hertog MGL, Feskens EJM, Hollam PCH, Katan MB, Kromhout D. Dietary antioxidant flavonoids and risk of coronary heart diseases. Lancet 1993; 342:1007-20.
• The Ayurvedic Formulary of India Part -I. Controller of Publications, Delhi, 2000; 8-9.
• Andallu B, Radhika B. Hypoglycaemic, Diuretic and Hypocholesterolemic effect of Winter cherry (Withania somnifera, Dunal) ischolar_main. Indian Journal of Experimental Biology 2000; 38:607-9.
• Budhiraja RD, Sudhir S. Review of biological activity of Withanolides. Journal of Scientific and Industrial research 1987; 46:488-91.
• Jadhav PD, Laddha KS. Estimation of gallic and ellagic acid from Terminalia chebula Retz. Indian Drugs 2004; 41(4):200- 06.
• Tuba AK, Ilhami G. Antioxidant and free radical scavenging properties of curcumin. Chemico-Biological Interactions 2008; 174:27-37.
• Mishra S. Bhaisazya Kalpana Vigyan, Chaukambha Surbharati Prakashan. Varanasi. 2005; 253-54.
• Alam M, Radhamani S, Ali U, Purushottam KK. Microbiological screening of dhataki flowers. Journal of Research in Ayurveda&Siddha 1984; 2(4):371-5.
• Rona G, Chapel CI, Balazs T, Gaudry R. An infarct like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat. Archives of Pathology 1959; 76:443-55.
• Tripathi KD. Essentials of Medical Pharmacology. 6th ed. New Delhi (India): Jaypee Brothers Medical Publishers Limited; 2008. p. 137-8, 537.
• Varley H. Practical Clinical Biochemistry. 4th ed. NY: William Heinemann; 1967. p. 161-2.
• Lamprecht W, Stan F, Weisser H, Heinz F. Determination of creatine phosphate and adenosine triphosphate with creatine kinase. In: Methods of Enzymatic analysis. Ed. HU Vergmeyer. NY: Academic Press; 1974. 1776-8.
• Mohun AF, Cook IGY. Simple methods for measuring serum levels of Glutamic oxaloacetic and Glutamic pyruvic transaminases in routine laboratories. Journal of Clinical Pathology 1957; 10 (4):394-9.
• Allain CC, Pool LS, Chan CS, Richmond W. Enzymatic determination of serum cholesterol. Clinical Chemistry 1974; 20:447-75.
• Friedewald WT, Levy RI, Fredrickson DS. Estimation of the Concentration of Low-density lipoprotein cholesterol in plasma, without use of the preparative ultra centrifuge. Clinical Chemistry 1972; 18 :499-502.
• Muller PH, Schmulling RM, Liebich HM, Eggstein M. A fully enzymatic triglyceride determination. Journal of Clinical Chemistry 1977; 15:457-64.
• Ohkawa H, Ohisi N, Yagi K. Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Analytical Biochemistry 1979; 95:351-8.
• Ellman GL. Tissue Sulphydril groups. Archives of Biochemistry&Biophysics 1959; 82:72-7.
• Nirmala C, Puvanakrishnan R. Isoproterenol induced myocardial infarction in rats; functional and biochemical alterations. Medicine Science and Research 1994; 22:575-7.
• Haenen GR, Veerman M, Bast A. Reduction of beta adrenoceptor functions by oxidative stress in heart. Free Radical Biology and Medicine 1990; 9:279-88.
• Tappia PS, Heta T, Dhalla NS. Role of oxidative stress in catecholamine induced changes in cardiac sarcolemmal Ca²⁺ transport. Archives of Biochemistry and Biophysics 2001; 377:85-92.
• Pederson TR. Low density lipoprotein cholesterol lowering is and will be the key to the future of lipid management. American Journal of Cardiology 2001; 87(5A):8B-12B.
• Bolden WE, Pearson TA. Raising low levels of High density lipoprotein cholesterol is an important target of therapy. American Journal of Cardiology 2000; 85(5):645-50.

• Antimicrobial Activity of Ashwagandharishta Prepared by Traditional and Modern Methods

Abstract Views :326  |  PDF Views:6

Authors

Preeti Tiwari ¹

Affiliations
1 Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar (U. P.), IN

Source

Asian Journal of Research in Pharmaceutical Sciences, Vol 4, No 3 (2014), Pagination: 115-117

Abstract

In the present investigation, different types of test preparations of Ashwagandharishta as Ashwagandharishta-T, Ashwagandharishta-M prepared by traditional and modern methods respectively and marketed Ashwagandharishta were evaluated for antimicrobial activity against common human pathogens. It was observed that all the test preparations of Ashwagandharishta exhibited significant zone of inhibition against selected common human pathogens. The results indicate that all the test preparations of Ashwagandharishta as Ashwagandharishta-T, Ashwagandharishta-M and marketed Ashwagandharishta might be used as natural drug for the treatment of several infectious diseases caused by these organisms.

Keywords

Ashwagandharishta-T, Ashwagandharishta-M, Antimicrobial Activity.

Full Text

     

References

• Prusti A, Mishra SR, sahoo S and Mishra SK. Antibacterial activity of Some Indian Medicinal Plants. Ethnobotanical Leaflets 2008; 12:227-230.
• Ebi GC and Ofoefule SI (2000) Antimicrobial Activity of Pterocarpus osun stems. Fitoterapia 71:433-435.
• Ncube NS, Afolayan AJ, Okoh A. Assessment Techniques of antimicrobial properties of natural compounds of plant origin:current methods and future trends. Africal Journal of Biotechnology 2008; 7(12):1797-1806.
• Ramya S, Govindaraji V, Kannan NK and Jayakumararaj R. In vitro evaluation of antibacterial activity using crude extracts of Catharanthus roseus L. Ethnobatanical Leaflets 2008; 12:1013-1018.
• Shariff Z U. Modern Herbal Therapy for Common Ailments. Nature Pharmacy Series, Spectrum Books Limited. Ibadan, Nigeria in Association with Safari Books (Export) Limited, United Kingdom, 2001; vol. 1, 94.
• The Ayurvedic Formulary of India Part-I. Controller of Publications, Delhi, 2000;8-9.
• Andallu B, Radhika B. Hypoglycaemic, Diuretic and Hypocholesterolemic effect of Winter cherry (Withania somnifera, Dunal) ischolar_main. Indian J Exp Biol 2000; 38:607-9.
• Budhiraja RD, Sudhir S. Review of biological activity of Withanolides. J Sci Ind Research 1987;46:488.
• Jadhav PD, Laddha KS. Estimation of gallic and ellagic acid from Terminalia chebula Retz. Indian Drugs 2004; 41(4):200-06.
• Tuba AK, Ilhami G. Antioxidant and free radical scavenging properties of curcumin. Chem Biol Interact 2008;174:27-37.
• Mishra S. Bhaisazya Kalpana Vigyan, Chaukambha Surbharati Prakashan. Varanasi. 2005;253-54.
• Alam M, Radhamani S, Ali U, Purushottam KK. Microbiological screening of dhataki flowers. J Res Ayurveda Siddha 1984;2(4):371-5.
• Bauer RW, Kirby MDK, Sherris JC and Turck M . Antibiotic susceptibility Testingby standard single disc diffusion method. American Journal of Clinical Pathology1966; 45:493-96.
• Sasidharan VK, Krishnakumar T and Manjula CB. Antimicrobial activity of Nine Common plants in Kerala, India. PJS 1998, 127 (1):59-67.

• Antihyperlipidemic Potential of Balarishta Prepared by Traditional and Modern Methods in High Fat Diet Induced Hyperlipidemic Rats

Abstract Views :257  |  PDF Views:2

Authors

Preeti Tiwari ¹

Affiliations
1 Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar, Uttar Pradesh, IN

Source

Asian Journal of Research in Pharmaceutical Sciences, Vol 4, No 1 (2014), Pagination: 7-11

Abstract

The objective of the present study was to evaluate the lipid peroxidation activity and related antihyperlipidemic activity of Balarishta-T and Balarishta-M prepared by traditional and modern methods and its marketed formulation in high fat diet induced hyperlipidemic rats. The antioxidant activity of Balarishta-T and Balarishta-M was increased in concentration dependent manner. Balarishta-T and Balarishta-M inhibited the ferrous sulphate induced lipid peroxidation in a dose dependent manner and showed inhibitory concentration (IC50) value 196.61 μg/ml and 201.72 μg/ml respectively. Oral administration of Balarishta-T and Balarishta-M for nine weeks at the dose of 2 ml/kg significantly reduced serum cholesterol, serum LDL and serum triglycerides while showed significant rise in serum HDL as compared to high fat diet fed control group. Marketed Balarishta also showed significant decrease in serum cholesterol, serum LDL, serum triglycerides and showed significant rise in serum HDL. Atorvastatin (1.2 mg/kg, p.o.) was used as standard antihyperlipidemic drug. Both types of Balarishta as Balarishta-T and Balarishta-M showed significant reduction in atherogenic index as compared to high fat diet fed control group which strongly supports antiatherosclerotic property of Balarishta.

Keywords

Balarishta, Lipid Per Oxidation, Atherogenic Index, Antihyperlipidemic Activity, Atorvastatin.

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References

• Tripathi. K.D. Essentials of Medical Pharmacology. 4^th Edition, published by Jaypee Brothers, New Delhi, 2002; 612-614.
• Singh N, Kapur KK and Singh SP. Mechanism of cardiovascular action of Terminalia arjuna . J Med Plant Res. 1982; 45:102-104.
• The Ayurvedic Formulary of India, Part-I. 2000, 1^st edition, The Controller of Publications, Delhi, 17.
• Prakash A, Verma RK, Ghosal S. Alkaloid constituents of Sida acuta, Sida humilis, S. rhombifolia and Sida spinosa. Planta Medica 1981;43:384-388.
• Ghosal S, Chauhan RR, Mehta R. Chemical constituents of Malvaceae, Alkaloids of Sida cordifolia. Phytotherapy Research 1975;14:830-832.
• Sutradhar RK, Rahman AM, Ahmad M, Bachar SC. Bioactive flavones of Sida cordifolia. Phytotherapy Letters 2008;1(4):179-182.
• Sutradhar RK, Rahman AM, Ahmad M, Bachar SC, Saha A. Analgesic and anti-inflammatory principle from Sida cordifolia Linn. Journal of Biological Sciences 2006;6(1):160-163.
• Budhiraja RD, Sudhir S. Review of biological activity of withanolides. Journal of Science and Industrial Research 1987;46:488.
• Andallu B, Radhika B. Hypoglycemic, diuretic and hypocholesterolemic effect of winter cherry (Withania somnifera, Dunal) ischolar_main. Indian Journal of Experimental Biology 2000;38:607-609.
• Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005.p. 253-254.
• Alam M, Radhamani S, Ali U and Purushottam KK. Microbiological Screening of Dhataki flowers. Journal of Research in Ayurveda and Siddha 1984; 2(4):371-375.
• Ohkawa H, Oshishi N and Yagi K. Assay for lipid peroxidation in animal tissues by thiobarbituricacid. Analytical Biochemistry 1979; 95:351.
• Lowery OH, Rosenbrough NJ, Farr AL and Randall RJ. Protein estimation with Folin phenol reagent. Biol Chem. 1951;193:265-275.
• Khanna AK, Chander R and Kapoor NK. Terminala arjuna: an Ayurvedic cardiotonic, Regulates lipid metabolism in Hyperlipidemic rats. Phytother Res. 1996;10: 663-665.
• Allain CC, Poon LS, Chan CS and Richmond W. Enzymatic Determination of Total Serum cholesterol. Clin Chem .1974;20::447-475.
• Friedewald WT, Levy RI and Fredrickson DS. Estimation of concentration of low density cholesterol in plasma without use of ultracentrifuge. J Clin Chem .1972;18: 449-502.
• Muller PH, Schmulling RM, Liebich HM and Eggstein M. A fully Enzymatic Triglyceride Determination. J Clin Chem. 1977;15:457-464.
• Anne SM, Ock SY, Debra AP, Andrew LW and Edwin NF. Inhibition of Human Low Density Lipoprotein oxidation in relation to composition of Phenolic antioxidants in Grapes (Vitis Vinifera). J Agri Food Chem. 1997;45 (5):1638-1643.
• S Renaud, and M De Lorgeril. Wine, alcohol, Platelets and the French Paradox for Coronary Heart Disease. The Lancet. 1992; 339: 1523-1526.
• Pederson TR. Low- density lipoprotein cholesterol lowering is and will be the key to the future of lipid management. American J Cardiol. 2001; 87(5A): 8B-12B.
• Boden WE and Pearson TA. Raising low levels of High Density Lipoprotein Cholesterol is an important target of therapy. American J cardiol. 2000; 85(5):645-650.

• Cardioprotective Activity of Draksharishta on Isoproterenol Induced Myocardial Infarction

Abstract Views :169  |  PDF Views:0

Authors

Preeti Tiwari ¹

Affiliations
1 Department of Pharmacognosy, Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar, Uttar Pradesh, IN

Source

Research Journal of Science and Technology, Vol 6, No 3 (2014), Pagination: 151-155

Abstract

The present study was designed to evaluate the cardio protective activity of Draksharishta-T, Draksharishta-M prepared by traditional and modern methods respectively and its marketed preparation on isoproterenol (ISO) induced myocardial infarction (MI) in albino rats. Wistar albino rats of either sex were randomly divided into 06 groups comprising 06 animals in each group as normal control, ISO control, pretreatment with Inderal*10 (10 mg/kg) per os, pretreatment with Draksharishta-T, M and its marketed preparation at the dose of 2 ml/kg per os per day for 30 days. MI was induced in all the groups except normal control, by administering ISO (85 mg/kg) intraperitoneally, on 29^th and 30^th day. On 31^st day, level of serum marker enzymes was determined and serum lipid profile was also measured. Then, animals were subsequently sacrificed; hearts were removed, weighed and immediately processed for biochemical studies. Pretreatment with Inderal*10 and all the test preparations of Draksharishta significantly prevented the ISO-induced adverse changes in the level of serum marker enzymes as creatine kinase (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and also improved serum lipid profile. All the test formulations pretreated groups showed significant increase in glutathione (GSH) content and significantly reduced malonyldialdehyde (MDA). Thus, experimental finding suggests that the cardio protective activity of Draksharishta-T, M and its marketed preparation may be due to an augmentation of endogenous antioxidants as GSH and inhibition of lipid peroxidation of cardiac membrane.

Keywords

Myocardial Infarction, Isoproterenol, Draksharishta.

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• Effect of Rate of Crude Oil Contamination on Index Properties and Engineering Properties of Clays and Sands

Abstract Views :110  |  PDF Views:0

Authors

Gupta Harsh ¹, Ashray Patel ¹, Bavishi Himanshu ¹, Preeti Tiwari ¹

Affiliations
1 Department of Civil Engineering, Indus University, Ahmedabad - 382115, Gujarat, IN

Source

Indian Journal of Science and Technology, Vol 9, No 30 (2016), Pagination: 

Abstract

Background: The recent advancements and industrial growth has adversely affected the environment which has direct or indirect impact on geotechnical properties of soil. One of the most perturbed sources of contamination is crude oil contamination, which basically takes place either due to accidental spillage of crude oil or through the industrial waste. This leads to alternation of index properties, chemical properties as well as engineering properties of soil. Methodology: Many researchers have worked on studying the impact of crude oil contamination on various types of soil. Present research work, focuses on not only evaluating the extent of alternation of the index properties (Specific Gravity, Liquid Limit, Plastic Limit, Shrinkage Limit) as well as engineering properties (Free Swelling Index) for Kaolinite Clay and fine grained sand due to crude oil contamination but also the effect of rate of crude oil contamination viz. 3%, 6% and 9% of crude oil contamination on both the soil types by performing geotechnical tests in accordance with IS Code:2720 part 3-1 (1980), part 5,6 (1985), part 40 (1977) respectively on non-contaminated soil samples as well as crude oil contaminated soil samples. For this purpose, the crude oil contaminated samples were prepared in the laboratory at water content equal to the liquid limit; simulating the in-situ conditions. Findings and Conclusion: The results thus obtained find its application in comparing the effect of crude oil on fine grained and coarse grained soil. The coarse grained soil (sand) due to its inherent structure and high permeability allows the penetration of crude oil at higher rate than that of fine grained soil (clay) which have low permeability and thus are less liable to get affected due to crude oil contamination. Increment in rate of crude oil contamination can be stated for the deterioration of geotechnical properties for both the soil types.

Keywords

Crude Oil-Sand and Clay Interaction, Engineering Properties, Index Properties.

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• Study of Effect of Hydrocarbon Contamination on Geotechnical Properties of Kaolnitic Clay and Expansive Soil through Linear Regression Analysis

Abstract Views :112  |  PDF Views:0

Authors

Gupta Harsh ¹, Ashray Patel ¹, Bavishi Himanshu ¹, Preeti Tiwari ¹

Affiliations
1 Department of Civil Engineering, Indus University, Ahmedabad - 382115, Gujarat, IN

Source

Indian Journal of Science and Technology, Vol 9, No 30 (2016), Pagination: 

Abstract

Background: Hydrocarbon contamination is one of the perturbed issues of concern in the current scenario of tremendous development in petroleum sector all over the world. Intrusion of such contaminants results in altering the engineering properties of soil which lie in the vicinity of such effected zones. Many research works have been done to evaluate the extent of deterioration of soil properties due to such contaminants on various soil samples. Methodology: The present research work focuses not only on hydrocarbon-clay and hydrocarbon-expansive soil interaction mechanism and effect of hydrocarbon on Kaolinite Clay and black cotton soil which is expansive in nature through laboratory tests viz. specific gravity test, Atterberg's limit test, free swell index test as per IS Code:2720 part 3-1 (1980), part 5,6 (1985) and part 40 (1977) respectively on non-contaminated as well as contaminated soil samples but also the effect of rate of hydrocarbon contamination on both the soil types and generate a comparative plot for the both so as to evaluate the behavior of different soil on subjection with hydrocarbon contamination. Soil sample was prepared by adding water equal to liquid limit of the soil and hydrocarbon in 3%, 6% and 9% of the total mass of soil sample taken followed by performing each test. Findings and Conclusion: Co-relations are obtained for each property w.r.t. % of hydrocarbon contamination for both the soil types through LRA. Using coefficient of regression as a parameter (R2 values) the co-relations obtained are validated. The conclusions were drawn stating that there is a remarkable alternation of swelling and shrinkage characteristics of black cotton soil due to hydrocarbon contamination. The extent of deterioration of geotechnical properties in expansive soils is more distinct then that in clays. Applications/Improvements: With the use of LRA, the co-relations can be validated and comparison for the suitability of a particular soil type at such contaminated zone can be determined.

Keywords

Hydrocarbon Contamination, Index and Engineering Properties, Linear Regression Analysis (LRA).

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• Phenolics and Flavonoids and Antioxidant Potential of Balarishta Prepared by Traditional and Modern Methods

Abstract Views :209  |  PDF Views:2

Authors

Preeti Tiwari ¹

Affiliations
1 Dr K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar (U. P.), IN

Source

Asian Journal of Pharmaceutical Analysis, Vol 4, No 1 (2014), Pagination: 5-10

Abstract

The objective of the present study was to estimate the total phenolic content as well as flavonoids in Balarishta-T and Balarishta-M prepared by traditional and modern methods respectively and in its marketed preparation and also to evaluate the antioxidant activity of these test preparations on two different in vitro antioxidant activity models. Total phenolic content was determined colorimetrically using Folin-Ciocalteu reagent and was found 0.1104 and 0.1098 %w/w gallic acid equivalent in Balarishta-T and Balarishta-M respectively. Total flavonoid content was determined by aluminium chloride method and was found 0.01548 and 0.01542 %w/w quercetin equivalent in Balarishta-T and Balarishta-M respectively. Super-oxide anion scavenging activity and lipid per-oxidation assay were carried out to evaluate the antioxidant potential of Balarishta-T and Balarishta-M. The antioxidant activity of Balarishta-T and Balarishta-M was found increased in concentration dependent manner in both the in vitro antioxidant activity models as super-oxide radical scavenging activity and lipid per-oxidation assay. Balarishta-T and Balarishta-M showed significant scavenging of super-oxide radical and showed IC50 65.75 μg/ml and 67.85 μg/ml respectively. Balarishta-T and Balarishta-M also inhibited the ferrous sulphate induced lipid per-oxidation in dose dependent manner and showed inhibitory concentration (IC50) 196.61 μg/ml and 201.72 μg/ml respectively. Marketed Balarishta also showed a rich concentration of total phenolics and flavonoids and showed dose dependent antioxidant activity in both the models. Thus, the results obtained in this study indicate that Balarishta-T and Balarishta-M can be a promising source of natural antioxidant.

Keywords

Total Phenolics, Flavonoids, Antioxidant Potential, Balarishta.

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• Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Ashwagandharishta Prepared by Traditional and Modern Methods

Abstract Views :194  |  PDF Views:0

Authors

Preeti Tiwari ¹, Rakesh K. Patel ²

Affiliations
1 Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut (U.P.), IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat,, ID

Source

Asian Journal of Pharmaceutical Analysis, Vol 3, No 4 (2013), Pagination: 147-152

Abstract

The objective of the present study was to estimate the total phenolic content as well as flavonoids in Ashwagandharishta-T and Ashwagandharishta-M prepared by traditional and modern methods respectively and in its marketed preparation and also to evaluate the antioxidant activity of these test preparations on two different in vitro antioxidant activity models. Total phenolic content was determined colorimetrically using Folin-Ciocalteu reagent and was found 0.1068 and 0.1065 %w/w gallic acid equivalent in Ashwagandharishta-T and Ashwagandharishta-M respectively. Total flavonoid content was determined by aluminium chloride method and was found 0.01366 and 0.01315 %w/w quercetin equivalent in Ashwagandharishta-T and Ashwagandharishta-M respectively. Super-oxide anion scavenging activity and lipid per-oxidation assay were carried out to evaluate the antioxidant potential of Ashwagandharishta-T and Ashwagandharishta-M. The antioxidant activity of Ashwagandharishta-T and Ashwagandharishta-M was found increased in concentration dependent manner in both the in vitro antioxidant activity models as super-oxide radical scavenging activity and lipid per-oxidation assay. Ashwagandharishta-T and Ashwagandharishta-M showed significant scavenging of super-oxide radical and showed IC50 91.32 and 99.39 μg/ml respectively. Ashwagandharishta-T and Ashwagandharishta-M also inhibited the ferrous sulphate induced lipid peroxidation in dose dependent manner and showed inhibitory concentration (IC50) 181.88 and 191.05 μg/ml respectively. Marketed Ashwagandharishta also showed a rich concentration of total phenolics and flavonoids and showed dose dependent antioxidant activity in both the models. Thus, the results obtained in this study indicate that Ashwagandharishta-T and Ashwagandharishta-M can be a promising source of natural antioxidant.

Keywords

Total Phenolics, Flavonoids, Antioxidant Potential, Ashwagandharishta.

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• Antimicrobial Activity of Draksharishta Prepared by Traditional and Modern Methods

Abstract Views :215  |  PDF Views:0

Authors

Preeti Tiwari ¹

Affiliations
1 Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar, Uttar Pradesh, IN

Source

Asian Journal of Pharmacy and Technology, Vol 4, No 3 (2014), Pagination: 131-133

Abstract

In the present investigation, different types of test preparations of Draksharishta as Draksharishta-T, Draksharishta-M prepared by traditional and modern methods respectively and marketed Draksharishta were evaluated for antimicrobial activity against commom human pathogens. It was observed that all the test preparations of Draksharishta exhibited significant zone of inhibition against selected common human pathogens. The results indicate that all the test preparations of Draksharishta as Draksharishta-T, Draksharishta-M and marketed Draksharishta might be used as natural drug for the treatment of several infectious diseases caused by these organisms.

Keywords

Draksharishta-T, Draksharishta-M, Antimicrobial Activity.

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• Diuretic Potential of Balarishta Prepared by Traditional and Modern Methods in Experimental Albino Rats

Abstract Views :192  |  PDF Views:1

Authors

Preeti Tiwari ¹

Affiliations
1 Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar (U. P.), IN

Source

Asian Journal of Pharmacy and Technology, Vol 4, No 1 (2014), Pagination: 13-16

Abstract

The objective of the present study was to evaluate the diuretic potential of Balarishta-T and Balarishta-M prepared by traditional and modern methods respectively and its marketed formulation in experimental rats using furosemide (10 mg/kg p.o.) as a standard diuretic drug. Oral administration of Balarishta-T, Balarishta-M and its marketed formulation at the dose of 2.0 ml/kg over a period of 5 h showed a significant increase in urine volume as compared to control group. Both types of Balarishta as Balarishta-T and Balarishta-M prepared by traditional and modern methods respectively and its marketed formulation showed significant increase in sodium, potassium and chloride level in urine sample as compared to control group. The maximum diuretic effect was produced by furosemide. Thus, both types of Balarishta as Balarishta-T and Balarishta-M and its marketed formulation showed significant diuretic, natriuretic and kaliuretic effects.

Keywords

Diuretic Potential, Furosemide, Balarishta, Natriuretic Effect, Kaliuretic Effect.

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• Evaluation of Diuretic Potential of Amritarishta Prepared by Traditional and Modern Methods in Experimental Albino Rats

Abstract Views :183  |  PDF Views:1

Authors

Preeti Tiwari ¹

Affiliations
1 Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar, Uttar Pradesh, IN

Source

Asian Journal of Research in Pharmaceutical Sciences, Vol 3, No 4 (2013), Pagination: 229-232

Abstract

The objective of the present study was to evaluate the diuretic potential of Amritarishta-T and Amritarishta-M prepared by traditional and modern methods respectively and its marketed formulation in experimental rats using furosemide (10 mg/kg p.o.) as a standard diuretic drug. Oral administration of Amritarishta-T, Amritarishta-M and its marketed formulation at the dose of 2.0 ml/kg over a period of 5 h showed a significant increase in urine volume as compared to control group. Both types of Amritarishta as Amritarishta-T and Amritarishta-M prepared by traditional and modern methods respectively and its marketed formulation showed significant increase in sodium, potassium and chloride level in urine sample as compared to control group. The maximum diuretic effect was produced by furosemide. Thus, both types of Amritarishta as Amritarishta-T and Amritarishta-M and its marketed formulation showed significant diuretic, natriuretic and kaliuretic effects.

Keywords

Diuretic Potential, Furosemide, Amritarishta, Natriuretic Effect, Kaliuretic Effect.

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• Evaluation of Cardioprotective Potential of Drakshasava in Induced Diabetic Condition

Abstract Views :181  |  PDF Views:0

Authors

Preeti Tiwari ¹, Rakesh K. Patel ², T. S. Eashwari ³

Affiliations
1 Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut, (U.P.), IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
3 IIMT College of Medical Sciences, Meerut, (U.P.), IN

Source

Research Journal of Pharmacognosy and Phytochemistry, Vol 5, No 2 (2013), Pagination: 87-93

Abstract

The objective of the present study was to evaluate the effect of Drakshasava- T and Drakshasava-M prepared by traditional and modern methods respectively and marketed Drakshasava on fasting blood glucose and serum lipid profile in alloxan induced diabetic rats. Oral administration of Drakshasava-T, Drakshasava-M and marketed Drakshasava (2 ml/kg p.o.) for 21 days caused a significant decrease in fasting blood glucose (FBG) and showed significant rise in blood glutathione level (GSH) in diabetic rats. Glibenclamide was used as a standard antidiabetic drug (10 mg/kg, p.o). These preparations also caused significant reduction in serum cholesterol, LDL and triglycerides and showed significant rise in serum HDL level in diabetic albino rats. Thus all these preparations were able to maintain the tested parameters near to the normal level significantly.

Keywords

Cardiovascular Risk, Blood Glucose, Anti-Diabetic Potential, Glutathione, Lipid Profile, Drakshasava, Alloxan.

Full Text

     

• Evaluation of Antihyperlipidemic Potential of Amritarishta Prepared by Traditional and Modern Methods in Hyperlipidemic Rats

Abstract Views :135  |  PDF Views:0

Authors

Preeti Tiwari ¹

Affiliations
1 Department of Pharmacognosy, Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar, Uttar Pradesh, IN

Source

Research Journal of Pharmacognosy and Phytochemistry, Vol 5, No 6 (2013), Pagination: 315-319

Abstract

The objective of the present study was to evaluate the lipid peroxidation activity and related antihyperlipidemic activity of Amritarishta-T and Amritarishta-M prepared by traditional and modern methods and its marketed formulation in high fat diet induced hyperlipidemic rats. The antioxidant activity of Amritarishta-T and Amritarishta-M was increased in concentration dependent manner. Amritarishta-T and Amritarishta-M inhibited the ferrous sulphate induced lipid peroxidation in a dose dependent manner and showed inhibitory concentration (IC₅₀) value 236.84 and 243.66 μg/ml respectively. Oral administration of Amritarishta-T and Amritarishta-M for nine weeks at the dose of 2 ml/kg significantly reduced serum cholesterol, serum LDL and serum triglycerides while showed significant rise in serum HDL as compared to high fat diet fed control group. Marketed Amritarishta also showed significant decrease in serum cholesterol, serum LDL, serum triglycerides and showed significant rise in serum HDL. Atorvastatin (1.2 mg/kg, p.o.) was used as standard antihyperlipidemic drug. Both types of Amritarishta as Amritarishta-T and Amritarishta-M showed significant reduction in atherogenic index as compared to high fat diet fed control group which strongly supports antiatherosclerotic property of Amritarishta.

Keywords

Amritarishta, Lipid Per Oxidation, Atherogenic Index, Antihyperlipidemic Activity, Atorvastatin.

Full Text

     

• Antimicrobial Activity of Drakshasava Prepared by Traditional and Modern Methods

Abstract Views :155  |  PDF Views:0

Authors

Preeti Tiwari ¹

Affiliations
1 Department of Pharmacognosy, Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar (U. P.), IN

Source

Research Journal of Pharmacognosy and Phytochemistry, Vol 6, No 3 (2014), Pagination: 126-128

Abstract

In the present investigation, different types of test preparations of Drakshasava as Drakshasava-T, Drakshasava-M prepared by traditional and modern methods respectively and marketed Drakshasava were evaluated for antimicrobial activity against commom human pathogens. It was observed that all the test preparations of Drakshasava exhibited significant zone of inhibition against selected common human pathogens. The results indicate that all the test preparations of Drakshasava as Drakshasava-T, Drakshasava-M and marketed Drakshasava might be used as natural drug for the treatment of several infectious diseases caused by these organisms.

Keywords

Drakshasava-T, Drakshasava-M, Antimicrobial Activity.

Full Text

     

• Total Phenolics and Flavonoids and Antioxidant Potential of Draksharishta Prepared by Traditional and Modern Methods

Abstract Views :184  |  PDF Views:0

Authors

Preeti Tiwari ¹, Rakesh K. Patel ²

Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711,Gujarat, IN

Source

Research Journal of Pharmacognosy and Phytochemistry, Vol 4, No 5 (2012), Pagination: 244-249

Abstract

The objective of the present study was to estimate the total phenolic content as well as flavonoids in Draksharishta-T and Draksharishta-M prepared by traditional and modern methods respectively and in its marketed preparation and also to evaluate the antioxidant activity of these test preparations on two different in vitro antioxidant activity models. Total phenolic content was determined colorimetrically using Folin-Ciocalteu reagent and was found 0.0967 and 0.0961 %w/w gallic acid equivalent in Draksharishta-T and Draksharishta-M respectively. Total flavonoid content was determined by aluminium chloride method and was found 0.01163 and 0.01129 %w/w quercetin equivalent in Draksharishta-T and Draksharishta-M respectively. Super-oxide anion scavenging activity and lipid per-oxidation assay were carried out to evaluate the antioxidant potential of Draksharishta-T and Draksharishta-M. The antioxidant activity of Draksharishta-T and Draksharishta-M was found increased in concentration dependent manner in both the in vitro antioxidant activity models as super-oxide radical scavenging activity and lipid per-oxidation assay. Draksharishta-T and Draksharishta-M showed significant scavenging of super-oxide radical and showed IC₅₀ 138.06 and 145.35 μg/ml respectively. Draksharishta-T and Draksharishta-M also inhibited the ferrous sulphate induced lipid peroxidation in dose dependent manner and showed inhibitory concentration (IC₅₀) 230.03 and 236.11 μg/ml respectively. Marketed Draksharishta also showed a rich concentration of total phenolics and flavonoids and showed dose dependent antioxidant activity in both the models. Thus, the results obtained in this study indicate that Draksharishta-T and Draksharishta-M can be a promising source of natural antioxidant.

Keywords

Total Phenolics, Flavonoids, Antioxidant Potential, Draksharishta.

Full Text

     

• Chances of Reduction in Cardiovascular Risk by Ashwagandharishta in Induced Diabetic Condition

Abstract Views :170  |  PDF Views:0

Authors

Preeti Tiwari ¹, R. K. Patel ²

Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384 001 (N. Gujarat), IN
2 Shri. S.K. Patel College of Pharmaceutical Education and Research, Kherva- 382711, (N. Gujarat), IN

Source

Research Journal of Pharmacognosy and Phytochemistry, Vol 2, No 2 (2010), Pagination: 171-174

Abstract

The objective of the study was to evaluate the chances of reduction in cardiovascular risk factors associated with diabetic conditions. Both types of Ashwagandharishta-T and Ashwagandharishta-M, were prepared by traditional and modern methods, respectively, and evaluated for fasting blood sugar , blood glutathione levels and serum biochemical parameters in alloxan induced diabetic rats. Both the Ashwagandharishta preparations were able to maintain the tested parameters near to normal level significantly.

Keywords

Cardiovascular Risk , Antidiabetic, Ashwagandharishta, Glutathione.

Full Text

     

• Development and Validation of Stability Indicating High-Performance Liquid Chromatographic Method for Determination of Pramipexole in Solid Dosage Forms

Abstract Views :211  |  PDF Views:0

Authors

Mayur G. Raval ¹, Preeti Tiwari ¹, C. N. Patel ¹

Affiliations
1 Shree Sarvajanik Pharmacy Collage, Near Arvind Baug , Mehsana-384001, Gujarat, IN

Source

Asian Journal of Research in Chemistry, Vol 4, No 9 (2011), Pagination: 1393-1397

Abstract

The Objective Of the current study was to develop a validated stability indicating high performance liquid chromatographic method for Pramipexole in solid dosage form. The method was validated by subjecting the drugs to forced decomposition under hydrolysis, Oxidation, photolysis, and thermal stress conditions prescribed in international Conference on Harmonization. The drug was successfully separated from major and minor degradation products on a reversed -phase Zorbex SB CN column by using Tri Ethyl Amine buffer (PH 7): Methanol (65:35%V/V) as the mobile phase with determination at 263 nm. The flow rate was 1 ml/min. The method was validated with respect to linearity, precision, accuracy, robustness. The response was linear over the range of 2-24 for Pramipexole. The recovery of the drugs from a mixture product was in the range of 99.60-101.84%. The utility of the procedure was verified by its application to marketed formulations that were subjected to accelerated stability studies.

Keywords

Pramipexole, Stability Assay, High Performance Liquid Chromatography, Validation, Mirapex.

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• Development and Validation of Spectrophotometric Method for Determination of Pramipexole in Solid Dosage Forms

Abstract Views :180  |  PDF Views:0

Authors

Mayur G. Raval ¹, Preeti Tiwari ¹, C. N. Patel ¹

Affiliations
1 Shree Sarvajanik Pharmacy Collage, Near Arvind Baug, Mehsana-384001, Gujarat, IN

Source

Asian Journal of Research in Chemistry, Vol 4, No 8 (2011), Pagination: 1340-1342

Abstract

The Objective Of the current study was to develop and validated UV spectrophotometric method for Pramipexole in solid dosage form. The method was validated by subjecting the drug to UV radiation. The drug was successfully quantified at 263 nm. The method was validated with respect to linearity, precision, accuracy, robustness. The response was linear over the range of 3-15 for Pramipexole. The recovery of the drugs from a mixture product was in the range of 99.00-101.46%. The utility of the procedure was verified by its application to marketed formulations. The specificity of the method was also checked with reference to placebo. The method was found to be specific also, hence other validation parameter were in the limit also.

Keywords

Pramipexole, Assay, UV Spectrophotometric, Validation, Mirapex.

Full Text

     

• Quantification of Quercetin and Rutin in Arjunarishta Prepared by Traditional and Modern Methods by Validated HPTLC Densitometry

Abstract Views :145  |  PDF Views:0

Authors

Preeti Tiwari ¹, Rakesh K. Patel ²

Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, IN

Source

Asian Journal of Research in Chemistry, Vol 4, No 6 (2011), Pagination: 1019-1024

Abstract

Arjunarishta, also known as Parthadhyarishta, is a polyherbal hydro alcoholic formulation and is advised as a choice of remedy in cardiovascular disorders. A simple, precise and accurate HPTLC method has been established for the determination of quercetin and rutin in Arjunarishta-T and Arjunarishta-M prepared by traditional and modern methods respectively and also in its marketed formulation. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ, specificity, robustness and ruggedness. The amount of quercetin in Arjunarishta-T, M and its marketed formulation was found to be 0.00260, 0.00234 and 0.00252% w/w respectively while rutin was found to be 0.00585, 0.00553 and 0.00568% w/w respectively. This is the first report for the quantification of quercetin and rutin in Arjunarishta by HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of quercetin and rutin from Arjunarishta.

Keywords

Arjunarishta, HPTLC, Validation, Quercetin.

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• Antimicrobial Activity of Amritarishta Prepared by Traditional and Modern Methods

Abstract Views :177  |  PDF Views:1

Authors

Preeti Tiwari ¹

Affiliations
1 Department of Pharmacognosy, Dr. K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar (U.P.), IN

Source

Asian Journal of Pharmaceutical Research, Vol 4, No 2 (2014), Pagination: 114-116

Abstract

In the present investigation, different types of test preparations of Amritarishta as Amritarishta-T, Amritarishta-M prepared by traditional and modern methods respectively and marketed Amritarishta were evaluated for antimicrobial activity against commom human pathogens. It was observed that all the test preparations of Amritarishta exhibited significant zone of inhibition against selected common human pathogens. The results indicate that all the test preparations of Amritarishta as Amritarishta-T, Amritarishta-M and marketed Amritarishta might be used as natural drug for the treatment of several infectious diseases caused by these organisms.

Keywords

Amritarishta-T, Amritarishta-M, Antimicrobial Activity.

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• Implementation of DNA Cryptosystem using Public and Private Key Cryptography

Abstract Views :300  |  PDF Views:1

Authors

Manoj Kumar Pandey ¹, Preeti Tiwari ²

Affiliations
1 Department of CSE, Swami Shri Swaroopanand Saraswati Mahavidyalaya, Hudco, Bhilai, IN
2 Department of CSE, Shri Shankracharya Technical College, Junwani, IN

Source

Networking and Communication Engineering, Vol 11, No 4 (2019), Pagination: 53-58

Abstract

Data security is one of the important aspects of network security so a encryption algorithm is needed to increase data security like DNA Cryptography. DNA cryptography is a fusion of biotech and Computer Science field. This paper mainly focuses on implementation of the DNA cryptosystem with the use of AES and keys management using RSA algorithm and also for verification of data at other side message digest SHA-256 has been used to achieve Authentication, integrity, confidentiality, Non-repudiation, Access control, availability & signature. The system has been implemented in java using NETBEANS IDE 8.2.

Keywords

Data Security, Advance Encryption Standard (AES), Rivest, Shamir, and Adelman (RSA), DNA Encryption (DNAE), Cryptography, Data Verification, SHA-256.

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References

• Jawahar Thakur, Nagesh Kumar “DES, AES and Blowfish: Symmetric Key Cryptography Algorithms Simulation Based Performance Analysis”, International Journal of Emerging Technology and Advanced Engineering, Volume 1, Issue 2, December 2011, ISSN 2250-2459
• ShraddhaSoni, HimaniAgrawal, Monisha Sharma “Analysis and Comparison between AES and DES Cryptographic Algorithm”, International Journal of Engineering and Innovative Technology (IJEIT), Volume 2, Issue 6, December 2012, ISSN: 2277-3754.
• Yunpeng Zhang and Liu He Bochen Fu (2012), “Research on DNA Cryptography, Applied Cryptography and Network Security”, Dr. Jaydip Sen (Ed.), ISBN: 978-953-51-0218-2, InTech, Available from: http://www.intechopen.com/books/applied-cryptography-and-network-security/research-on-dna-cryptography
• Radu Terec, Mircea-Florin Vaida, Lenuta Alboaie, Ligia Chiorean, “DNA Security using Symmetric and Asymmetric Cryptography”, pp 34-51, Available at https://www.researchgate.net/publication/230771223
• Yogesh Kumar, Rajiv Munjal, Harsh Sharma, “Comparison of Symmetric and Asymmetric Cryptography with Existing Vulnerabilities and Countermeasures”, International Journal of Computer Science and Management Studies, Vol. 11, Issue 03, Oct 2011.
• Posch, K. C., Posh, R., “Designing a new encryption method for optimum parallel performance”, IEEE First International Conference on Algorithms and Architectures for Parallel Processing, Brisbane, Qld., Australia 1995.
• Vinay kumar Pant, Ashutosh kumar, “DNA Cryptography: An New Approach to Secure Cloud Data”, International Journal of Scientific & Engineering Research, Volume 7, Issue 6, June-2016, pp 890-895
• Vaida MF., Terec R., Alboaie L. (2011), “Alternative DNA Security Using BioJava”, In: Cherifi H., Zain J.M., El-Qawasmeh E. (eds) Digital Information and Communication Technology and Its Applications, DICTAP 2011, Communications in Computer and Information Science, volume 166. Springer, Berlin, Heidelberg
• Tatiana, Hodorogea, Vaida, Mircea, Monica, Borda, Cosmin, Streletchi. (2008), “A Java Crypto implementation of DNA Provider featuring complexity in theory and practice”, pp 607 – 612,10.1109/ITI.2008.4588479
• Harneet Singh, Karan Chugh, Harsh Dhaka, A. K. Verma, “DNA based Cryptography: An Approach to Secure Mobile Networks”, International Journal of Computer Applications (0975 - 8887) Volume 1 – No. 19, pp 77-80
• Snehal Javheri, Rahul Kulkarni, “Secure DataCommunication Using DNA based Cryptography in Mobile Adhoc Network”, International Journal of Science and Research (IJSR), Volume 3, Issue 9, September 2014, pp 1504-1508.
• http://technet.microsoft.com/en-us/library/cc962035.aspx.
• RaziehMokhtarnameh, NithiapidaryMuthuvelu, Sin Ban Ho, Lan Chai “A Comparison Study on Key Exchange-Authentication Protocol”, Faculty of Information Technology, Multimedia University, Jalan Multimedia, 63100 Cyberjaya, Selangor, Malaysia, International Journal of Computer Applications (0975 – 8887)Volume 7– No.5, September 2010.
• Manoj kumar pandey, Prateeksha Pandey, “An Effective and Secure Key Management Scheme in Multiphase Cryptosystem”, CiiT International Journal of Networking and Communication Engineering ISSN: 0974 – 9616 Volume 6, Issue 6, August 2014, pp 237-242.