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M. Prabakaran ¹, V. Thennarasu ¹, R. Ayeswariya Mangala ¹, R. Bharathidasan ¹, N. Chandrakala ², N. Mohan ³

Affiliations
1 Sri Gowri Biotech Research Academy, 5, Nagai Road, Thanjavur-613 001, TN, IN
2 Department of Zoology, K.N.G. Arts College for Women (Autonomous), Thanjavur-613 007
3 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai-600025, IN

Abstract

Three fungal species namely, Aspergillus fumigatus, Penicillium chrysogenum and Verticillium terrestre, isolated from sugarcane field soil, revealed the extracellular production of amylase, cellulase and lipase. Improvement in elucidation of these enzymes was achieved by developing mutants after exposure to UV light.

Keywords

Amylase, Cellulase, Lipase, Extra Cellular Enzyme, Fungi, A. Fumigatus, P. Chrysogenum, V. Terrestre

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S. Suganya ¹, R. Bharathidasan ¹, G. Senthilkumar ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

Extraction of essential oils from locally available plant Coriandrum sativam (L.) was carried out using steam distillation followed by ether extraction. Dried and purified extracted oils were screened for their antibacterial activity of both gram positive (Staphylococcus aureus) and gram negative (Enterobacter aerogenes, Klebsiella pneumoniae, Vibrio cholerae and Salmonella typhi) bacterial strains by Agar well diffusion method. Coriandrum sativam shows antibacterial activity against the above given five bacterial strains. GC-MS was done on Coriandrum sativam oil. These results support that this plant oil can be used to cure bacterial infections and may also have role as pharmaceuticals and preservatives.

Keywords

Coriandrum sativam, GC-MS, Essential Oil, Antibacterial Activity and Extraction.

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K. Kaviyarasi ¹, A. Cholarajan ², R. Bharathidasan ², P. Madhanraj ², A. Panneerselvam ²

Affiliations
1 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
2 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN

Abstract

PGPR offers an environmentally sustainable approach to crop production and health. Many members of genera pseudomonas sp. perform beneficial effects on plants. Among them P. putida and P. fluorescens are fluorescent pseudomonas that represent Significant part of these Members. The rhizosphere soil was collected from cultivated plots. P. fluorescence was isolated and identified through morphologicaland hemical tests and it was treated against some plant pathogens. The biocontrol potential of P. fluorescens against plant pathogen such as A. terreus sp, Circinella sp, Verticillium sp, was assessed by dual plate technique. The seed treatment was made on four plants namely Hibiscus esculentus, Oryza sativa, Capsicum annum, Spinacia olerace. Pot culture techniques was applied for the growth of plants along with p. fluorescence inoculums (100ml) and chemical fertilizer (10g).The plant height, ischolar_main length, ischolar_main biomass, shoot biomass were also estimated. The results were discussed.

Keywords

PGPR, Pseudomonas fluorescence.

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S. Gnanavel ¹, R. Bharathidasan ¹, R. Mahalingam ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 BG and Research Dept of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

The antimicrobial effect of some selected Indian medicinal plants Strychnos nux-vomica and Cassia angustifolia, were selected. The solvents used for the extraction of plant leaves were n-butanol, methanol and distilled water. The invitro antimicrobial activity was performed by agar well diffusion method. The most susceptible gram-positive bacteria was Staphylococcus and Salmonella while other most susceptible gram negative bacteria was klebsiella pneumonia and fungal species namely Aspergillus terreus, Aspergillus flavus and Aspergillus niger. The n-butanol extract of plant Strychnos nux-vomica and Cassia angustifolia, inhibit all the bacterial and fungal strains investigated. The most active extract was compared with the standard antibiotics, penicillin, streptomycin and ampicillin with 100mg disc. In the present study Strychnos nuxvoimica and Cassia angustifolia showed maximum inhibition against the fungal and bacterial organisms tested. Thus the n-butanol plant extracts could be used to control the above microbes.

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M. Mariyappan ¹, R. Bharathidasan ¹, R. Mahalingam ¹, P. Madhanraj ², A. Panneerselvam ¹, V. Ambikapathy ¹

Affiliations
1 P.G.Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur. DT, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621212, Tamil Nadu, IN

Abstract

The bacterial organisms were isolated from drinking water (Bacillus, Borchothrix, Clavibacter sp, Ancylobacter sp, and Brevi bacterium). Selected Indian medicinal plants Cardiospermum halicacabum and Melothria heterophylla were selected for antibacterial studies. The solvents used for the extraction of plant ischolar_mains were n- butanol, ethyl acetate and distilled water. The invitro antibacterial activity was performed by agar well diffusion method. The most susceptible Gram-Postive bacteria was Bacillus sp, Brevibacterium sp, and the most susceptible Gram-negative bacteria was Borchothrix sp, Clavibacter sp, and Ancylobacter sp. The extracts of plant Cardiospermum halicacabum and Melothria heterophylla inhibited the growth of the bacterial strains investigated. The most active extracts was compared with the standard antibiotics, penicillin, Streptomycin and Ampicillin 100mg/disc). The results obtained in the present study suggest that Cardiospermum halicacabum and Melothria heterophylla could be used in treating diseases caused by the test organisms. The results are discussed in detail.

Keywords

Medicinal Plants, Antibacterial Activity, Aqueous Extract, N-Butanol Excretory Ethyalcetate Extract.

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