Informatics Publishing Limited

Kumaraswamy Gandla ¹, R. Lalitha ¹, Sadhana Bommakanti ¹, R. Suthakaran ¹, K. Pallavi ²

Affiliations
1 Department of Pharmaceutical Analysis, Teegala Ram Reddy College of Pharmacy, Meerpet, Hyderabad- 505172, Telangana, IN
2 SSJ College of Pharmacy, Vattinagulpalli, Hyderabad, Telangana, IN

Abstract

A Simple, specific and sensitive an isocratic simultaneous Estimation by RP-HPLC analytical Method were developed and validated for the quantification Albendazole and Praziqantel. Quantification was achieved by using the mobile phase Phosphate Buffer pH 4.0 :Acetonitrile and Methanol in the ratio of 20:70:10 Inertsil ODS,C-18,250×4.6mm ID, 5μm Particle size was used as stationary phase. The flow rate was 1.0 ml/min. Measurements were made at a wavelength of 238nm. The average retention times for Albendazole and Praziqantel was found to be 2.713 and 4.770min. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear from 60-180μg/ml and5-15μg/ml for Albendazole and Praziqantel respectively. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of Albendazole and Praziqantel.

Keywords

Albendazole And Praziqantel, RP-HPLC Method, Isocratic Elution Tablet Dosage Forms.

Full Text

     

Kumaraswamy Gandla ¹, T. Harika ¹, R. Lalitha ¹

Affiliations
1 Department of Pharmaceutical Analysis, Teegala Ram Reddy College of Pharmacy, Meerpet, Hyderabad- 505172, Telangana, IN

Abstract

The present work deals with the development of a precise, accurate, simple, specific, reliable and less time consuming RP-HPLC method for the estimation of Aceclofenac and Tramadol tablets .The chromatographic separation was achieved on a Inertsil C18 ODS(4.6 x 250mm, 5μm) with a mobile phase combination of methanol and water (50:50) v/v at a flow rate of 1.0 ml/min, and the detection was carried out by using PDA detector at 290 nm. Ambient column temperature has maintained. The total run time was 10mins.The retention time of Tramadol and Aceclofenac were found to be 2.9 min. and 3.5 min. respectively. The performance of the method was validated according to the present ICH guidelines.

Keywords

RP-HPLC, Aceclofenac, Tramadol, PDA Detection Tablet Dosage Forms.

Full Text