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S. Gnanavel ¹, R. Bharathidasan ¹, R. Mahalingam ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 BG and Research Dept of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

The antimicrobial effect of some selected Indian medicinal plants Strychnos nux-vomica and Cassia angustifolia, were selected. The solvents used for the extraction of plant leaves were n-butanol, methanol and distilled water. The invitro antimicrobial activity was performed by agar well diffusion method. The most susceptible gram-positive bacteria was Staphylococcus and Salmonella while other most susceptible gram negative bacteria was klebsiella pneumonia and fungal species namely Aspergillus terreus, Aspergillus flavus and Aspergillus niger. The n-butanol extract of plant Strychnos nux-vomica and Cassia angustifolia, inhibit all the bacterial and fungal strains investigated. The most active extract was compared with the standard antibiotics, penicillin, streptomycin and ampicillin with 100mg disc. In the present study Strychnos nuxvoimica and Cassia angustifolia showed maximum inhibition against the fungal and bacterial organisms tested. Thus the n-butanol plant extracts could be used to control the above microbes.

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M. Mariyappan ¹, R. Bharathidasan ¹, R. Mahalingam ¹, P. Madhanraj ², A. Panneerselvam ¹, V. Ambikapathy ¹

Affiliations
1 P.G.Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur. DT, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621212, Tamil Nadu, IN

Abstract

The bacterial organisms were isolated from drinking water (Bacillus, Borchothrix, Clavibacter sp, Ancylobacter sp, and Brevi bacterium). Selected Indian medicinal plants Cardiospermum halicacabum and Melothria heterophylla were selected for antibacterial studies. The solvents used for the extraction of plant ischolar_mains were n- butanol, ethyl acetate and distilled water. The invitro antibacterial activity was performed by agar well diffusion method. The most susceptible Gram-Postive bacteria was Bacillus sp, Brevibacterium sp, and the most susceptible Gram-negative bacteria was Borchothrix sp, Clavibacter sp, and Ancylobacter sp. The extracts of plant Cardiospermum halicacabum and Melothria heterophylla inhibited the growth of the bacterial strains investigated. The most active extracts was compared with the standard antibiotics, penicillin, Streptomycin and Ampicillin 100mg/disc). The results obtained in the present study suggest that Cardiospermum halicacabum and Melothria heterophylla could be used in treating diseases caused by the test organisms. The results are discussed in detail.

Keywords

Medicinal Plants, Antibacterial Activity, Aqueous Extract, N-Butanol Excretory Ethyalcetate Extract.

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