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Rajeshwari, R.
- Detection and Characterization of Wolbachia in Cotesia plutellae (Kurdjumov) (Hymenoptera: Braconidae), a Parasitoid of the Diamond Back Moth Plutella xylostella (Linn.)
Authors
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 3 (2011), Pagination: 213-216Abstract
Cotesia plutellae (Kurdjumov) is an indigenous, larval endoparasitoid that attacks mid instar larvae of the diamond back moth (DBM). Although the parasitoid is distributed widely, not all local populations appear to be equally effective in controlling the DBM. Bacterial endosymbionts may play regulatory role in determining their efficiency. Field collected C. plutellae populations from cauliflower fields were assessed for the prevalence of the bacterial endosymbionts. Bacterial endosymbionts in the genus Wolbachia were detected in the populations obtained from Hoskote (Karnataka) and Thirupathi (Andhra Pradesh). PCR amplification using specific primers for Wolbachia revealed 528 and 518 bp for the populations, respectively. Sequencing of the Wolbachia surface protein, wsp, revealed Wolbachia infection to be related to Wolbachia endosymbiont of Cotesia glomerata outer surface protein, wsp gene (Genbank Accession No. AB094202) with maximum identity of 99% with BLAST search of NCBI. The sequence was submitted to the GenBank with the Accession No. JF421566. The detection of Wolbachia in the parasitoid signifying its role in biological manipulations of the parasitoid for enhanced efficiency is discussed.Keywords
Wolbachia, Characterization, Cotesia plutellae, Cabbage, Plutella xylostella.Full Text
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- Assessment of Genetic Variation in Cotesia flavipes Cameron (Hymenoptera: Braconidae) Populations as Revealed by Mitochondrial Cytochrome Oxidase Gene Sequences
Authors
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 23, No 3 (2009), Pagination: 249-253Abstract
The cytochrome oxidase gene (COI) was employed to assess the genetic variation in different populations of Cotesia flavipes. Partial sequences of the COI gene for the populations from Bangalore, Hyderabad, New Delhi and Shimla were analyzed to assess the homology and the usefulness of this genetic region for phylogenetic studies. PCR using COI-F and COI-R primers amplified a product of approximately 550 bp which was similar for all the four populations. Populations were by and large similar in the COI gene sequenced and there was no variation with our sequences and those of sequences worldwide. The multiple alignments were performed for four populations which revealed similarity of the partial COI gene sequences. Comparative analysis of partial sequences of COI gene produced a phylogenetic tree. Phylogenetic analysis revealed that all our populations were in a single clade with high boot strap value, suggesting highest similarity.Keywords
Cotesia flavipes, Cytochrome Oxidase, Genetic Variation.Full Text
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- Occurrence of Chrysoperla zastrowi arabica Henry et al. (Neuroptera: Chrysopidae), a Cryptic Song Species of Chrysoperla (Carnea-group), in India
Authors
1 Biological Control, Post Bag No. 2491, Bellary Road, Hebbal, Bangalore - 560024, Karnataka, IN
2 Biological Control, Post Bag No. 2491, Bellary Road, Hebbal, Bangalore - 560024, Karnataka., IN
Source
Journal of Biological Control, Vol 22, No 1 (2008), Pagination: 143-147Abstract
The Chrysoperla species (carnea-group) widely used in India in augmentative biological control programmes was found to be Chrysoperla zastrowi arabica Henry et al. The acoustic profile of the mating song of this species was analyzed and found to match that of C. zastrowi arabica, the song species characterized earlier as 'Cc5 generator'. The correct taxonomic name for this species remains to be determined as it is morphologically identical to C. sillemi (Esben-Petersen), a species already known from India. Molecular characterization of the species was also done and the DNA sequence of the ITS-2 region was deposited in GenBank (Accession No. DQ 825504, as that of C. sillemi). The implications of this finding are discussed.Keywords
Chrysopidae, Carnea-group, Chrysoperla zastrowi Arabica, C. sillemi, India, Molecular Characterization, Song Analysis.Full Text
- Effectiveness of Structured Teaching Programme on Knowledge and Techniques Regarding Metered Dose Inhalers (MDI) Among Bronchial Asthma Patients in Selected Hospitals of Mangalore Taluk
Authors
1 Department of Medical-Surgical Nursing, Alva’s College of Nursing, Moodbidri, IN
Source
International Journal of Advances in Nursing Management, Vol 4, No 3 (2016), Pagination: 223-234Abstract
Objectives of the study:
The objectives of the study were to:
*assess the pre-test knowledge and techniques regarding metered dose inhalers among bronchial asthma patients.
*evaluate the effectiveness of structured teaching programme on knowledge and techniques regarding metered dose inhalers among bronchial asthma patients.
*find out the correlation between knowledge and techniques regarding metered dose inhalers among bronchial asthma patients.
*find out the association between the pre-test knowledge score and selected demographic variables among bronchial asthma patients.
*find out the association between pre-test techniques score and selected demographic variables among bronchial asthma patients.
Methods:
An evaluative approach was used for the study to test the effectiveness of structured teaching programme prepared for bronchial asthma patients. The conceptual framework adopted for the study was based on Wiedenbach’s helping art of clinical nursing theory (1964).
To obtain content validity of the tool, the prepared tool along with the problem statement, objectives, operational definition, inclusion and exclusion criteria, and structured interview schedule along with the demographic proforma, structured teaching programme were submitted to nine experts. Seven were from the field of medical surgical nursing speciality and two were doctors in the field of pulmonology and medicine.
To ensure the reliability, the tool was administered to 6 bronchial asthma patients in selected hospitals of Mangalore Taluk (annexure 1). Who fulfilled the sampling criteria. Split half technique was used to assess the internal consistency. The reliability was tested by applying Karl Pearson’s coefficient of Spearman Brown Prophecy formula. The reliability quotient was found to be 0.80 for structured interview schedule and 0.86 for observational checklist, which indicated that the tools are reliable.
Non probability purposive sampling was used to select the sample for the study. The sample size was 60. Pilot study was conducted to find out the feasibility of the study. Data collected from the sample were analysed by descriptive and inferential statistics.
Significant findings of the study:
*The findings of this study demonstrated that 48.3% belonged to age group of 41-50, majority of the subjects
(78.3%) were male, maximum subjects (36.7%) were Hindus, majority of them belonged to nuclear family, most of them (33.3%) had secondary education, maximum (48.35) were in the field of business, most (43.3%) of their family monthly income was 5,001-10,000 rupees, most (45%) of them had illness >5 years, most (23.3%) of them were using MDI since 4 years, many of them (51.7%) had the family history of bronchial asthma, and majority of them (73.3%) had the source of information.
*The mean percentage of pre-test knowledge score was 37.5%, whereas post-test knowledge score was 91.4%.
*The mean percentage of pre-test technique score was 66.3% and post-test technique score was (91.8%).
*The ‘t’ value computed between mean pre-test and post-test knowledge score is statistically significant (t(cal) = 35.33, table value t(59) = 1.67, p<0.05) and ‘t’ value computed between pre-test and post-test technique score is statistically significant(t(cal) =15.57 table value t(59) = 1.67, p <0.05). this shows that there is significant difference between the mean pre-test and post-test knowledge score and mean pre-test and posttest score of bronchial asthma patients regarding MDI, it shows that Structured Teaching Programme (STP) was effective in increasing the knowledge and technique of bronchial asthma patients regarding MDI. Thus it showed that as the knowledge increased technique improved.
*There was negative correlation between pre-test knowledge and technique (r(cal) = -0.180, table value r(58)=0.236;p<0.05), whereas there was positive correlation between pre-test knowledge and technique (r(cal)=0.839, table value r(58)=0.236;p<0.05) of bronchial asthma patients regarding MDI.
*The findings of the study revealed that there was no association between pre-test knowledge score and selected demographic variables.
*The findings of the study revealed that there was no association between pre-test technique score and selected demographic variables.
Conclusion:
The findings of the study showed that the structured teaching programme was effective in all the areas in improving the knowledge and technique of bronchial asthma patients regarding MDI.
Keywords
Bronchial Asthma Patients, Structured Teaching Programme, Metered Dose Inhalers.
- Don't Let Media Bully Your Body:Using Positive Media Images to Change Body Image Perception among Young Adolescents
Authors
1 Department of Psychology Kamala Nehru College, University of Delhi, Delhi, IN
2 Department of Psychology, Kamala Nehru College, University of Delhi, Delhi, IN
Source
IAHRW International Journal of Social Sciences Review, Vol 6, No 4 (2018), Pagination: 653-658Abstract
Of all the components that play a role in determining a person's self image, the perception that she/he has of their body and physical appearance is one of the more essential considerations. Such perceptions are not formed in isolation, but in response to socio-cultural stimuli present around us. One of the more impactful of these is the media. To explore this relationship, this study aims to uncover the role of media on body image. For this purpose, two short videos depicting body positivity and unrealistic beauty standards present in the media and two standard questionnaires were used to assess media influence on a sample of 100 people between the ages of 18 and 24. Results revealed that while desires to alter their appearance for the better existed among the women, a significant relationship existed only between positive media representation and not for the unrealistic beauty standards.Keywords
Body Image, Media Representation, Perception, Body Image States Scale, Body Parts Dissatisfaction Scale.References
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