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Venkatnarayanan, R.

• Hepatoprotective Activity of Borreria hispida on Paracetamol Induced Liver Damage

Abstract Views :539  |  PDF Views:0

Authors

D. Benito Johnson ¹, C. Senthil Kumar ¹, R. Rajesh ¹, R. Venkatnarayanan ¹, VK Mohammed Ansar ¹

Affiliations
1 Department of Pharmacology, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamil Nadu, IN

Source

Research Journal of Pharmacy and Technology, Vol 6, No 1 (2013), Pagination: 61-65

Abstract

Hepatocyte are the functional cells of the liver and perform a wide range of metabolic, secretory and endocrine functions. Hepatotoxicity implies chemical-driven liver damage. The liver plays a major role in transforming and clearing chemicals and is susceptible to the toxicity from these agents. Certain medicinal agents, when taken in overdoses and sometimes even when introduced within therapeutic ranges, may injure the organ. Other chemical agents, those used in laboratories and industries, natural chemicals and herbal remedies can also cause hepatotoxicity. Borreria hispida seed flavonoid-rich fraction possesses free radical scavenging and antioxidant activity both in vitro and in vivo Borreria hispida Linn has been in use in the Indian system of medicine. Various part of the plant are useful in the treatment of antifertility, appetite, Bleeding in child birth, body ache, Gum trouble, scabies and skin disease, Stomach compliance, Ulcers, hepatitis, Wounds, head ache and tooth ache. The hepatotoxicity is induced by the paracetamol overdose, and the methanolic extract of Borreria hispida shows a good reduction of hepatotoxicity.

Keywords

Borreria hispida, Paracetamol, Hepatocyte, Hepatotoxicity

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References

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• Acute and Subacute Toxicity study of Milnacipran Hydrochloride in Wistar rats by Oral Route

Abstract Views :295  |  PDF Views:0

Authors

D. Benito Johnson ¹, R. Suresh ¹, Prakash Rao Prathima ¹, R. Venkatnarayanan ¹, P. M. Ashir Ahammad ¹

Affiliations
1 Department of Pharmacology, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamil Nadu, IN

Source

Research Journal of Pharmacology and Pharmacodynamics, Vol 5, No 1 (2013), Pagination: 51-57

Abstract

Toxicity of a substance is nothing but unwanted or series of adverse events that was initiated after administration of particular chemical, physical or biological agent. Acute toxicity study aim is to determine the occurred toxic manifestation of the administered test substance after expose to animals in one or more doses for a period of 14 days. The study provides the information or determination of therapeutic index, i.e. T.I. = LD50/ ED 50. Sub-acute toxicity testing evaluates the toxic effects of drug on repeated exposure and also provides the information on delayed and cumulative effect of the chemicals on the tissues or other biochemical mechanisms Depression is one of the most common psychiatric disorders. The symptoms of depression are often subtle and unrecognized both by patients and by physicians. Major depression remains difficult to treat, despite the wide array of registered antidepressant. Milnacipran is indicated for the treatment of major depressive disorder and management of fibromyalgia. Milnacipran inhibits norepinephrine and serotonin reuptake in a 3:1 ratio, in practical use this means a balanced (equal) action upon both transmitter.

Keywords

Milancipran, Depression, Acute Toxicity, Sub-Acute Toxicity

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References

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• Wound Healing and Anti Bacterial Activity of the Leaves Extracts of Jasminum grandiflorum Linn

Abstract Views :243  |  PDF Views:1

Authors

T. Raja Sekharan ¹, M. Syam Mohan ², P. Natarajan ³, A. S. William ³, Arputha Sundar ³, A. Thanga Thirupathi ³, R. Venkatnarayanan ⁴

Affiliations
1 Department of Pharmaceutics , Sankaralingam Bhuvaneswari College of Pharmacy, Anaikuttam – 626 130, Sivakasi, Tamil Nadu, IN
2 UPM Makna Cancer Research Lab, Serdang - 43400, Selangor, MY
3 Sankaralingam Bhuvaneswari College of Pharmacy, Anaikuttam – 626 130, Sivakasi, Tamil Nadu, IN
4 RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641 402, Tamil Nadu, IN

Source

Research Journal of Pharmacology and Pharmacodynamics, Vol 2, No 6 (2010), Pagination: 388-391

Abstract

Petroleum ether, Chloroform, Ethanol and Aqueous extracts of Jasminum grandiflorum Linn leaves were tested for wound healing and antibacterial activity. Investigation were undertaken to screen the wound healing activity of different extracts of the leaves of Jasminum grandiflorum Linn on excision wound models in albino rats. The aqueous extracts shows significance wound healing activity when compared to the standard (Povidone iodine ointments). Other extract also shows wound healing activity but the wound contraction was less when compared to aqueous extracts and standard. For Salmonella typhi the zone of inhibition for petroleum ether extract is equal and for chloroform extract it is more active compared to that of the standard (Ciprofloxacin disc 5mcg/disc). Ethanolic extract is more active against Staphylococcus aureus than other microorganisms. Aqueous extract is more effective against Streptococcus pyogen and less active against Proteus when compared to the standard.

Keywords

Jasminum Grandiflorum Linn; Antibacterial Activity, Wound Healing Activity.

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References

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• Zhao GQ, Xia JJ and Dong JX. Glycosides from leaves of Jasminum grandiflorum var officinale, Acta Pharmaceutica Sinica, 2007, 42: 1066-9.
• Villegas LF, Fernandez ID, Maldonado H, Torres R, Zavaleta A, Vaisberg AJ and Hammond GB. Evaluation of the woundhealing activity of selected traditional medicinal plants from Peru. J Ethnopharmacol, 1997, 55: 193-200.
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• In Vivo Anti-Snake Venom Activity of Methanol Extract of Leaves of Orthosiphon stamineus in Mice

Abstract Views :274  |  PDF Views:0

Authors

C. Maheswari ¹, R. Venkatnarayanan ², P. Babu ³, C. S. Kanadasamy ⁴

Affiliations
1 Department of Pharmacology, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, IN
2 Department of Pharmacognosy, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamilnadu, IN
3 Department of Pharmaceutical Chemistry, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore Tamilnadu, IN
4 Department of Pharmacognosy, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore Tamilnadu, IN

Source

Research Journal of Pharmacology and Pharmacodynamics, Vol 6, No 3 (2014), Pagination: 126-128

Abstract

Aim of this study was to evaluate the in vivo anti-snake venom activity of leaves of Orthosiphon stamineus were studied against Cobra (Naja Naja) venom. The in vivo study was carried out by using Swiss albino mice in modifying the lethal effect of the test dose of the Cobra venom. In in vivo model the effectiveness of the extract was evaluated by oral administration of two different doses (200 and 400 mg/kg) of the methanolic extract of the leaves of Orthosiphon stamineus 5 minutes prior to the injection of the venom and the percentage of mortality was observed. The extract markedly decreased the percentage of mortality in venom induced toxicity in mice at the dose of 400mg/kg b.w which indicates the significant anti-snake venom activity of the plant there by justifying its use in the indigenous system of medicine. The present study has confirmed the ethnomedical use of the plant for the treatment of snake bite.

Keywords

Snake venom, Naja Naja, Orthosiphon stamineus, Leaves, Mice.

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References

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• A Validated Reversed Phase HPLC-Method for the Determination of Aceclofenac and Tizanidine in Tablets

Abstract Views :308  |  PDF Views:159

Authors

R. Siva Kumar , P. Senthil Kumaran , P. Kumar Nallasivan , W. D. Sam Solomon , R. Venkatnarayanan

Source

Asian Journal of Pharmaceutical Research and Health Care, Vol 2, No 1 (2010), Pagination: 84-94

Abstract

A new simple, accurate, precise and reproducible Reverse Phase-High Performance Liquid Chromatographic method has been developed for the simultaneous estimation of Aceclofenac and Tizanidine in tablet dosage forms using C₁₈ column (Ineretsil, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of acetonitrile, methanol and 20 mM phosphate buffer adjusted to pH 3.5 in ratio of (40:30:30 v/v) with Ultraviolet-Visible detection at 230 nm. The method was linear over the concentration range for aceclofenac120-280 μg/ml and for tizanidine 2-40 μg/ml. The recoveries of Tizanidine and Aceclofenac were found to be in the range of 99.45-100.61% and 99.56-101.32% respectively. The validation of method was carried out using International Conference on Harmonization-guidelines. The described High Performance Liquid Chromatographic method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.

Keywords

Simultaneous Estimation, RP - HPLC, Aceclofenac, Tizanidine, ICH Guidelines.

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• Derivatized HPTLC Method for Simultaneous Estimation of Glucosamine and Ibuprofen in Tablets

Abstract Views :228  |  PDF Views:149

Authors

W. D. Sam Solomon , Rahul A. Kumar , P. R. Vijai Anand , R. Sivakumar , R. Venkatnarayanan

Source

Asian Journal of Pharmaceutical Research and Health Care, Vol 2, No 2 (2010), Pagination: 156-162

Abstract

A selective, precise and derivatized HPTLC method has been developed for the simultaneous estimation of Glucosamine and Ibuprofen in tablet formulation. In this method standard and sample solutions of Glucosamine and Ibuprofen were applied on pre-coated silica gel 60F₂₅₄ TLC plate, and developed using Propanol-Ethyl acetate-Ammonia-Water (4: 3: 2: 1 v/v), as mobile phase and derivatized using Iodine vapor. The drugs on the plate were scanned at 254 nm. The dynamic linearity range was 5-25 μg/spot for Glucosamine and 2-10 μg/spot for Ibuprofen. The method was validated for precision, recovery and reproducibility.

Keywords

Simultaneous Estimation, HPTLC, Glucosamine and Ibuprofen.

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• Evaluation of Standards of Some Selected Cosmetic Preparations

Abstract Views :190  |  PDF Views:136

Authors

T. Akelesh , R. Siva Kumar , R. Jothi , Vijairajan , P. Arulraj , R. Venkatnarayanan

Source

Asian Journal of Pharmaceutical Research and Health Care, Vol 2, No 4 (2010), Pagination: 302-306

Abstract

The aim of the present work is to analyze the standards of marketed cosmetic products which are largely consumed in day to day life of the people. The cosmeceutical should be tested for efficacy to ensure a proven skin benefit and also to substantiate marketing claims. The work was done by keeping the ideas of Bureau of Indian Standards to analyze the cosmetic products. The evaluation for the following cosmetics such as tooth pastes (Colgate, Closeup, Pepsodent, Vicco and Anchor) and face powders (Ponds, Eva, Fa, Cuticura and Spinz) are performed for their quality. All the marketed tooth pastes and face powders which had been evaluated complied with the standards specified by Bureau of Indian Standards. Hence all the selected marketed tooth pastes and selected face powders were found to be of good quality.

Keywords

Cosmetic Products, Bureau of Indian Standards, Tooth Paste, Face Powder.

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• Formulation and Evaluation of Mycophenolate Mofetil Capsules

Abstract Views :181  |  PDF Views:0

Authors

T. Akelesh ¹, R. Ramya ², P. Venkatesh ², Divya Jacob ², R. Venkatnarayanan ²

Affiliations
1 Department of Pharmaceutics, R.V.S College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, IN
2 Department of Pharmaceutics, R.V.S. College of Pharmaceutical Sciences, Trichy Road, Coimbatore-641402, IN

Source

Research Journal of Pharmaceutical Dosage Form and Technology, Vol 3, No 4 (2011), Pagination: 148-151

Abstract

Mycophenolate Mofetil is an immunosuppressant drug used in treatment of systemic lupus erythematosus and in organ transplantation in doses ranging from 180mg to 500mg. Preformulation studies including micromeritic properties of pure drug and drug excipient compatibility studies using DSC were performed. Formulation was done by Wet Granulation process. The physical properties of the blend like LOD, Bulk density, Compressibility index, Hausner ratio, and Sieve analysis were tested and the finished product were evaluated for Invitro drug release profile, Weight variation, Thickness, and Stability studies. The dissolution profile of the formulated capsules was compared with Innovator product. The release rate was identical to that of Innovator product.

Keywords

Mycophenolate Mofetil, Immunosuppressant, Wet Granulation Process, Pregelatinized Starch.

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• Pharmacognostical and Preliminary Phytochemical Screening the Leaves of Jasminum grandiflorum Linn

Abstract Views :149  |  PDF Views:0

Authors

T. Raja Sekharan ¹, M. Syam Mohan ², R. Venkatnarayanan ³, A. Thanga Thirupathi ⁴

Affiliations
1 Sankaralingam Bhuvaneswari College of Pharmacy, Anaikuttam – 626 130, Sivakasi, Tamil Nadu, IN
2 UPM, Makna Cancer Research Lab, Serdang – 43400, Selangor, MY
3 RVS college of Pharmaceutical Sciences, Sulur, Coimbatore-641 402, IN
4 Sankaralingam Bhuvaneswari College of Pharmacy, Anaikuttam – 626 130, Sivakasi, Tamil Nadu, IN

Source

Research Journal of Pharmacognosy and Phytochemistry, Vol 2, No 6 (2010), Pagination: 438-440

Abstract

This study deals with the detailed pharmacognostical evaluation of the leaves of Jasminum grandiflorum linn. The macroscopical, microscopical, physicochemical analysis and phytochemical analysis will help to differentiate the drug from other species.

Keywords

Jasminum grandiflorum, Macroscopical, Microscopical.

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• Simultaneous Estimation of Amoxycillin Trihdrate and Dicloxacillin Sodium in Formulation by UV-Spectroscopy

Abstract Views :182  |  PDF Views:1

Authors

M. Manu ¹, W. D. Sam Solomon ¹, R. Venkatnarayanan ¹

Affiliations
1 Department of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore - 641402, Tamilnadu, IN

Source

Asian Journal of Research in Chemistry, Vol 4, No 2 (2011), Pagination: 241-243

Abstract

A simple UV- Spectrophotometric method has been developed for simultaneous estimation of Amoxycillin Trihydrate and Dicloxacillin Sodium from Pharmaceutical dosage forms. Methanol: water (1:1) was used as solvent. The method involves the measurement of absorbance at two wavelengths 290 nm (λmax for Amoxycillin) and 274nm (λmax for Dicloxacillin). The linearity lies between 2-10 μg/ml for Amoxycillin and10-50 μg/ml for Dicloxacillin. The accuracy and precision of the methods were determined and validated statiscally. The method showed good reproducibility and recovered with %RSD less than 2. The method are found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of simultaneous estimation of Amoxycilin and Dicloxacillin in bulk and combined dosage form.

Keywords

Simultaneous, UV-Spectrophotometric, Amoxycilin, Dicloxacillin.

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• Chemo Preventive Activity of Triumfetta rhomboidea in 7, 12-Dimethylbenz (A) Anthracene Induced Breast Cancer in Sprague–Dawley Rat Model

Abstract Views :150  |  PDF Views:1

Authors

R. Suresh ¹, D. Benitojohnson ¹, C. Maheswari ¹, R. Venkatnarayanan ², R. Manavalan ³

Affiliations
1 Department of Pharmacology, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, IN
2 RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, IN
3 Department of Pharmaceutics, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, IN

Source

Research Journal of Pharmacy and Technology, Vol 10, No 3 (2017), Pagination: 687-692

Abstract

The chemopreventive potential was assessed by monitoring the tumour incidence, total no of tumours and tumour volume and also by analyzing the level of biochemical markers such as 17-β estradiol (E2), TBARS and antioxidants during DMBA induced mammary carcinoma. A single subcutaneous injection of DMBA (25mg/kg) produced mammary carcinoma in female Sprague-Dawley rats. Oral administration of 100mg/kg and 200mg/kg of TRM to DMBA treated rats significantly prevented the tumour incidence, total no of tumours, tumour volume and brought back the above said biochemical markers to normal. The present study confirmed the chemopreventive activity of leaves of Triumfetta rhomboidea in mammary carcinoma

Keywords

Mammary Carcinoma, Triumfetta rhomboidea, Leaves, DMBA, Antioxidants.

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• RP-HPLC Method for Simultaneous Estimation of Tramadol HCl and Paracetamol Bulk Drug and its Combined Dosage Form

Abstract Views :154  |  PDF Views:0

Authors

Rajesh Shukla ¹, R. Sivakumar ¹, P. R. Vijayanand ¹, P. Kumar Nallasivan ¹, R. Venkatnarayanan ¹

Affiliations
1 Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamilnadu, IN

Source

Asian Journal of Research in Chemistry, Vol 3, No 3 (2010), Pagination: 552-554

Abstract

This work is concerned with application of simple, accurate, precise and highly selective reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of Tramadol HCl and Paracetamol in combined dosage form. Chromatographic separation was achieved isocratically at room temperature on phenomenex C18 column (250×4.6 mm) with a mobile phase composed of 5% Tri-fluoroaceticacid in water:Acetonitrile:Methanol in the ratio of 70:20:10% v/v/v at flow rate of 1.0 ml/min. Detection is carried out using a UV detector at 254 nm. The retention time of Tramadol HCl and Paracetamol was found to be 3.890±0.5 min and 1.990±0.5 min respectively. The method was found to be linear in the range of 0.1-10 μg/ml with mean recovery of 98.96% for Tramadol HCl and 99.11% for Paracetamol. The correlation coefficients for all components are close to 1. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed method was successfully applied for simultaneous determination of Tramadol HCl and Paracetamol in routine analysis.

Keywords

Tramadol HCl, Paracetamol, RP-HPLC.

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• Derivatized HPTLC Method for Simultaneous Estimation of Glucosamine, Vitamin C and Vitamin E in Tablets

Abstract Views :182  |  PDF Views:0

Authors

W. D. Sam Solomon ¹, Rahul A. Kumar ¹, P. R. Vijai Anand ¹, R. Venkatnarayanan ¹

Affiliations
1 Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, IN

Source

Asian Journal of Research in Chemistry, Vol 3, No 2 (2010), Pagination: 329-331

Abstract

An accurate, precise and derivatized HPTLC method has been developed for the simultaneous estimation of Glucosamine, Vitamin C and Vitamin E in tablet formulation. In this method standard and sample solutions of Glucosamine, Vitamin C and Vitamin E were applied on pre-coated silica gel 60F₂₅₄ TLC plate, and developed using ethanol:Acetic acid (9:1 v/v), as mobile phase and derivatized using Iodine vapor. The drugs on the plate were scanned at 500 nm. The dynamic linearity range was 20-100 μg/spot for Glucosamine, 2-10 μg/spot for Vitamin C and 0.2-1 μg/spot for Vitamin E. The method was validated for precision, accuracy and reproducibility.

Keywords

Simultaneous Estimation, HPTLC, Glucosamine, Vitamin C and Vitamin E.

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• Computer Aided Docking Studies on Antiviral Drugs for Bird Flu

Abstract Views :176  |  PDF Views:0

Authors

R. Siva Kumar ¹, Shaik Nafeez Basha ¹, P. Kumar Nallasivan ¹, W. D. Sam Solomon ¹, R. Venkatnarayanan ¹

Affiliations
1 Dept. of Pharmaceutical Chemistry, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, (T.N.), IN

Source

Asian Journal of Research in Chemistry, Vol 3, No 2 (2010), Pagination: 370-373

Abstract

The Protein-Ligand interaction plays a significant role in structural based drug designing. The highly pathogenic influenza A virus subtype H5N1 virus is an emerging avian influenza virus that has been causing global concern as a potential pandemic threat. It is often referred to simply as "bird flu" or "avian influenza". In our research work we have taken influenza A virus H5N1 receptor. The receptor was docked to the commercially available drugs zanamivir and oseltamivir and the energy value obtained are as follows; zanamivir (-231.74) and oseltamivir (-243.74) using the HEX docking software. We tried to improve the binding efficiency and steric compatibility of zanamivir against N5N1 receptor. Several modifications were made to the probable functional groups which were interacting with the receptor molecule. Analogs of this drug molecule were prepared using ACD ChemSketch and docked using HeX docking software. Zanamivir analog 3 and oseltamivir analog 5 were detected with significant energy values and probable lead molecules. The Modified drugs was sketched using Chemsketch were found to be better than the conventional drugs available. Further from this work we can improve the steric compatibility and then ADME properties of the Analogs can be analyzed using Inslico ADME tools available.

Keywords

Bird Flu, Chemsketch, Docking, Hex, Rasmol.

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• Simultaneous RP-HPLC Estimation of Nitazoxanide and Ofloxacin in Tablet Dosage Forms

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Authors

R. Siva Kumar ¹, P. Kumar Nallasivan ¹, S. Saravanakumar ¹, C. S. Kandasamy ¹, R. Venkatnarayanan ¹

Affiliations
1 Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, IN

Source

Asian Journal of Research in Chemistry, Vol 2, No 1 (2009), Pagination: 43-45

Abstract

Reverse Phase HPLC method for the determination of nitazoxanide and ofloxacin in bulk and tablet formulations. The determination was carried out by using Phenomenex C18 column with 0.24% sodium lauryl sulphate:acetonitrile:acetic acid (pH-4.0) (58:40:02) as the mobile phase. The flow rate was 1.5 ml/min and the eluent was monitored at 295 nm. The Retention time of nitazoxanide and ofloxacin were 2.2 and 5.4 respectively. Linearity for the nitazoxanide and ofloxacin were found in the range of 400-600 μg/ml and 160-240 μg/ml respectively. The method was reproducible, with good resolution between nitazoxanide and ofloxacin and can be use for routine analysis.

Keywords

RP-HPLC, Nitazoxanide and Ofloxacin.

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