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Gaurav Patel ¹, Sanjay Patel ², Rajendra Mehta ³, Mandev Patel ¹

Affiliations
1 Nootan Pharmacy College, Visnagar - 384 315, Gujarat, IN
2 Shri B.M. Shah College of Pharmaceutical Education & Research, Modasa, Gujarat, IN
3 A. R. College of Pharmacy, Vallabh Vidyanagar - 388 120, Anand, Gujarat, IN

Abstract

A method for simultaneous estimation of Amlodipine besylate and Hydrochlorothiazide in tablet dosage form has been described. The method is based on UV-Spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 236.17 nm (emax of Amlodipine besylate) and 268.58 nm (emax of Hydrochlorothiazide) in methanol. Linearity was obtained in the range of 6-33 µg/ml for Amlodipine besylate and 3-33 μg/ml for Hydrochlorothiazide. The method allows rapid analysis of binary pharmaceutical formulation with accuracy. Limit of Quantification and Limit of Detection for Amlodipine besylate at 236.17 nm was found to be 2.1 and 0.6, respectively and for Hydrochlorothiazide 0.9 and 0.28, respectively. Limit of Quantification and Limit of Detection for Amlodipine besylate at 268.58 nm was found to be 1.0 and 0.31, respectively and for Hydrochlorothiazide 0.15 and 0.045, respectively. Results of analysis for this method were validated statistically and were found satisfactory.

Keywords

Amlodipine Besylate (AML), Hydrochlorothiazide (HCT), UV-Spectrophotometry.

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Nidhi Dubey ¹, Nitin Dubey ², Rajendra Mehta ³, Ajay Saluja ³

Affiliations
1 School of Pharmacy, Devi Ahilya Vishwavidyalaya, Indore [M.P], IN
2 College of Pharmacy, IPS Academy, Indore [M.P], IN
3 A. R. College of Pharmacy, Vallabh Vidyanagar [Gujarat], IN

Abstract

A simple, sensitive, rapid and selective high-performance liquid chromatographic (HPLC) method has been developed and validated for the analysis of catechin in marketed Ayurvedic Taila (oil) formulations containing Acacia catechu. Chromatography of methanolic: 0.1% formic acid (7:3, v/v) extracts of these formulations was performed on C₁₈ (5 μm×25 cm×4.6 mm i.d) column using isocratic mobile phase consisting of methanol:acetonitrile:water (40:15:45, v/v/v) containing 1.0% acetic acid at a flow rate of 0.5 ml/min and SPD-10 A_VP photodiode array (PDA) UV-Visible detector. The analytical marker, catechin, was quantified at 279 nm. The retention time of catechin was about 3.27 min. The linear regression analysis data for the calibration plot showed a good linear relationship with correlation coefficient of 0.9988 in the concentration range of 15 to 90 μg/ml for catechin with respect to peak area. The limit of detection and limit of quantitation values were found to be 0.5 μg/ml and 1.7 μg/ml respectively. Repeatability of the method was found to be 0.62 RSD. Recovery values from 99.73 to 100.22% indicate excellent accuracy of the method. The developed HPLC method is accurate, precise, and cost-effective, and it can be successfully applied for the determination of catechin in marketed ayurvedic oil formulations containing Acacia catechu.

Keywords

Acacia catechu, HPLC, Oil, Quantitation.

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