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Rani, T.
- Assessment of Genetic Diversity in Jatropha Curcas (L.) Germplasm from India Using Rapd Markers
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Indian Forester, Vol 138, No 6 (2012), Pagination: 491-497Abstract
Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic diversity among populations of Jatropha curcas (L.) from different agro-climatic regions of India. Out of 305 amplified bands obtained with 30 primers, 291 were found polymorphic. The polymorphisms were scored and used in band-sharing analysis to identify genetic relationship. Evaluated accessions were grouped into two main clusters except MP-020 from Ratlam (Madhya Pradesh) was out crouped from rest of accessions at a similarity coefficient of 0.50. Based on Jaccard's coefficient of similarity values, the maximum similarity was found between accessions MP-022 and MP-031 (0.95). Molecular diversity among the accessions was low at a level of 30 per cent, indicating the need of widening the genetic base of J.curcas through various means.Keywords
Jatropha curcas, accessions, RAPD, genetic diversity
- RAPD Analysis of Induced Mutants of Jatropha (Jatropha curcas)
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Affiliations
1 CCS Haryana Agricultural University, Hisar-125004, IN
2 Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, DE
3 Central Soil and Water Conservation Research and Training Institute, Dehradun, IN
1 CCS Haryana Agricultural University, Hisar-125004, IN
2 Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, DE
3 Central Soil and Water Conservation Research and Training Institute, Dehradun, IN
Source
Indian Forester, Vol 139, No 12 (2013), Pagination: 1097-1104Abstract
Genetic variability in Jatropha curcas was induced by different doses (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 kR) of gamma-rays. Gamma radiation induced earliness in flowering and the plants set flowers earlier than that of normal (control), which took longer duration of 327 days for flowering. The improved reproductive and yield parameters such as days taken to first flowering, flowering population, male to female ratio and seed yield per plant were recorded in 25 kR dose and seed germination in 5 and 10 kR treated seeds. Molecular characterization of induced mutants (M1 generation) with 47 RAPD primers showed 65.27 per cent polymorphism. The variability created by gamma rays ranged from 9 to 28 per cent. The 50 kR mutant was found to be the most diverse from control followed by 25 kR mutant. Thus, this integrated approach can be used for carrying out the mutation-assisted breeding and subsequent selection of desired mutants using molecular markers in J. curcas.Keywords
Jatropha curcas, Gamma-rays, Induced Mutagenesis, RAPDReferences
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