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K. Shailaja ¹, R. Revathi ¹, V. S. Saravanan ¹

Affiliations
1 Department of Pharmaceutical Analysis, The Erode College of Pharmacy and Research, Erode- 638112, IN

Abstract

A simple, accurate and precise reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous quantitative estimation of Emtricitabine (EMT) and Tenofovir disoproxil fumerate (TDF) in tablet dosage form. Quantitation was carried on an ACE (150 mm x 4.6mm, 5μ) column using gradient composition of 0.01 M citric acid monohydrate buffer pH 4.5 as mobile phase A and methanol as mobile phase B at a flow rate of 1.0 mL/min with detection at 260 nm. Determination of the different validation parameters such as precision, linearity, accuracy, specificity, robustness, limit of detection, limit of quantification and filter validation was done. The system suitability parameters such as retention time, theoretical plates and tailing factor were found to be 3.181min, 13438, 0.14 and 6.090min, 48847, 1.05 respectively for EMT and TDF. The detector response is linear from 16 μg/mL to 64 μg/mL for EMT and 24 μg/mL to 96 μg/mL TDF. The limit of detection and limit of quantification was 0.101, 0.189 μg/mLand 0.306, 0.575 μg/mL for EMT and TDF respectively. The percentage recovery for average of three different concentrations (50, 100 and 150%) was 101.1, 100.6, 99.8 and 99.7, 99.4, 99.6% for EMT and TDF respectively.The high percentage of recovery and low % RSD confirms the suitability of the method for the simultaneous estimation of EMT and TDF in tablet dosage form.The method could effectively separate the drugs from their degradation products, it can be regarded as stability indicating method.

Keywords

RP-HPLC, Simultaneous Estimation, EMT, TDF, Forced Degradation Studies

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R. Revathi ¹, V. S. Saravanan ¹, T. Ethiraj ², Jhansi Lakshmi Marreddy ¹

Affiliations
1 Department of Pharmaceutical Analysis, The Erode College of Pharmacy and Research, Erode, Tamil Nadu, IN
2 Department of Pharmaceutics, The Erode College of Pharmacy and Research, Erode, Tamil Nadu, IN

Abstract

A simple reverse phase liquid chromatographic method was developed and validated as per the ICH guidelines for the quantitative determination of candesartan cilexetil in pharmaceutical dosage forms. The mobile phase consisted of 0.02 M mono basic potassium phosphate buffer: acetonitrile: triethyl amine (40:60:0.2) and adjust pH to 6.0 with ortho phosphoric acid. The eluent was monitored at 254 nm, at a flow rate of 2 mL/min and retention time was observed at 9.153 min. The linearity was observed from 0.08071-0.24213 mg/mL with R2= 0.998. The LOD and LOQ were found to be 0.00005 mg/mL and 0.00017 mg/mL respectively. This method was also validated and can be successfully applied for routine analysis.

Keywords

Candesartan Cilexetil, RP-HPLC, Validation.

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S. Manokaran ¹, V. S. Saravanan ², Y. Bhargavi ²

Affiliations
1 Dept. of Pharmacognosy, The Erode College of Pharmacy, Veppampalayam, Vallipurathan Palayam (PO), Erode – 638 112 Tamilnadu, IN
2 The Erode College of Pharmacy, Tamilnadu, IN

Abstract

Hepato- toxicity is a common toxicity happening during the treatment of chronic disease like tuberculosis, diabetics. Prunus persica belongs to Rosaceae is a plant growing in temperate region has laxative , sedative, anticancer property and also consist of glycosides, flavonoids, anthocyanins, vitamins. It also posses hepato-protactent property. To prove the activity scientifically the ethanolic extract of Prunus persica was studied against paracetamol induced hepatotoxicity in albino rats. The extract when evaluated at dose of 100mg/ kg was found to significantly reduce the elevated levels of serum lysosomal enzyme Sgpt, Sgot, Alp and Bilirubin when compared with standard silymarin.

Keywords

Prunus persica, Paracetamol, Albino Rats, Serum Enzymes.

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S. Manokaran ¹, V. S. Saravanan ², T. M. Kulanthavel ², C. T. Kumarrapan ²

Affiliations
1 Dept. of Pharmacognosy, The Erode College of Pharmacy, Veppampalayam, Vallipurathan Palayam (PO), Erode – 638 112, Tamilnadu, IN
2 The Erode College of Pharmacy, Erode, IN

Abstract

The hydro-alcoholic extract of Cucurbita maxima pulp shows the haematological activity against phenyl hydrazine induced anaemic rat. The hydro alcoholic extract of 150 mg/kg and 300 mg/kg are shown significant action against the anaemia, when compared to the 10 mg/kg of phenylhydrazine induced group after 4 weeks of treatment. The parameters determined are haemaglobulin content, RBC, WBC.

Keywords

Haematinic Activity, Cucurbita maxima Pulp, Phenylhydrazine, Haematological Parameters.

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V. S. Saravanan ¹

Affiliations
1 Department of Microbiology, Indira Gandhi College of Arts and Science, Kathirkamam, Puducherry 605 009, IN

Abstract

Recently, the University Grants Commission, India has provided a structure of the model curriculum for B Sc (Honours) programme in Microbiology. It is based on the choice-based credit system pattern aiming to improve the academic standards and quality of microbiology education in the country. The structure of the programme has been divided into core courses, discipline-centric elective courses, generic electives, ability enhancement compulsory courses and skill enhancement elective courses. Most of the basic papers related to microbiology are well covered under the core courses and care has been taken to include the recently published books as references. However, certain terms that could bring confusion among the teachers and students have been included, and some topics that are important to microbiology are missing. The subject contents offered under the generic electives seem to be mere repetition of the core courses. Here, I provide a few suggestions to improve the standards of the above-mentioned programme.

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A. Rama Chandra Reddy ¹, K. Roshna ¹, P. Mohanraj ¹, T. M. Kulanthavel ¹, V. S. Saravanan ¹, V. Ganesh ¹

Affiliations
1 Department of Pharmaceutical Analysis, The Erode College of Pharmacy, Veppampalayam, Erode-638112, TN, IN

Abstract

A new simple, sensitive, precise and economical spectrophotometric method of analysis for a bulk drug was developed and validated. The method employed methanol as solvent. The drug was then estimated at 270 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r²=0.999 in the concentration range 0.5-3.0 μg/ml. The mean value of correlation coefficient, slope and intercept were 0.999, 0.0332 and 0.0002 respectively. The method was validated for precision, accuracy and recovery studies. LOD and LOQ for norethisterone was found to be 0.01987 (μg/ml) and 0.06024 (μg/ml) respectively. The method has been successfully applied in the analysis of marketed formulations.

Keywords

Norethisterone, UV Spectrophotometric Analysis.

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J. Vanitha ¹, A. Saravana Kumar ², M. Ganesh ², V. S. Saravanan ¹

Affiliations
1 Department of Analytical Chemistry, Erode College of Pharmacy, Erode-112, IN
2 Department of Pharmaceutical Bio-Technology, Nandha College of Pharmacy, Erode-52, IN

Abstract

Climbazole is a member of the azole class of anti-mycotic, is often incorporated in several pharmaceutical forms and in shampoo formulation it is known to be effective against fungal infection on the scalp. This paper describes a method to quantify climbazole in shampoo formulation by cylinder plat assay method and the HPLC method. The test organism used for the agar diffusion assay was candida albicans ATCC10231. Three different concentrations of climbazole were used for the diffusion assay. A mean zone diameter was obtained for each concentration. A standard curve was obtained by plotting the three values derived from the zone diameter. A prospective validation of the method showed that the method was linear (r=0.9766), precise (RSD=2.45%) and accurate. The results obtained by the cylinder plate method was statistically evaluated by analysis of variance (ANOVA) and the results obtained indicate that there is no significant different between these two methods.

Keywords

Climbazole Shampoo, Microbiological Assay, Cylinder-Plate, Candida albicans.

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