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Zarna Dedania ¹, Ronak Dedania ¹, G. Vidhyasagar ¹, Bhavna Patel ², Chetan Ramolia ³, Vaishali Karkhanis ⁴

Affiliations
1 Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, IN
2 Ramanbhai Patel College of Pharmacy, Changa, Gujarat, IN
3 Veeda, Ahmedabad, Gujarat, IN
4 A. R. College of Pharmacy, Vallabh, Vidhyanagar, IN

Abstract

A simple, precise, sensitive, rapid and reproducible HPTLC method for the simultaneous estimation of the omeprazole and ondansetron in tablets was developed and validated. This method involves separation of the components by HPTLC on precoated silica gel G₆₀F254 plate with solvent system of methanol: ethyl acetate: toluene(2:6:2% v/v) and detection was carried out densitometrically using a UV detector at 280 nm in absorbance mode. This system was found to give compact spots for omeprazole (Rf value of 0.68±0.02) and for ondansetron (Rf value of 0.17±0.02). Linearity was found to be in the range of 1000-3500 ng/spot and 1000-3500 ng/spot for omeprazole and ondansetron respectively. The limit of detection and limit of quantification for omeprazole were 123.42 and 374.01 ng/spot and for ondansetron were 134.63 and 407.97 ng/spot, respectively. The method was found to be beneficial for the routine analysis of combined dosage form.

Keywords

Simultaneous Estimation, HPTLC, Omeprazole, Ondansetron.

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Zarna Dedania ¹, Ronak Dedania ¹, Vaishali Karkhanis ², G. Vidya Sagar ¹, Meeta Baldania ¹, N. R. Sheth ³

Affiliations
1 Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, Gujarat, IN
2 A.R. College of Pharmacy, Vallabh, Vidhyanagar, Gujarat, IN
3 Department of Pharmaeutical Sciences, Saurastra University, Rajkot, Gujarat, IN

Abstract

A RP-HPLC method is developed for simultaneous estimation of omeprazole and ondansetron in combined tablet dosage form. Omeprazole is a proton pump inhibitor and in the treatment of gastro-oesophageal reflux disease (GERD), peptic ulcer and Zollinger-Ellison syndrome. Ondansetron is used as selective 5-HT3 receptor antagonist and used in the management of nausea and vomiting induced by cytotoxic chemotherapy and radio therapy and also post operative nausea and vomiting. The mobile phase used was a combination of Methanol: Acetonitrile (90:10). The detection of the combined dosage form was carried out at 218 nm and a flow rate employed was 0.5 ml/min. The retention time for omeprazole and ondansetron was found to be 5.39 and 11.08 min respectively. Linearity was obtained in the concentration range of 4 to 20 μg/ml of omeprazole and 4 to20 μg/ml of ondansetron with a correlation coefficient of 0.997 and 0.9967. Detector consists of photodiode array detector; the reversed phase column used was RP-C18 (2.27μm size, 250 mm´4.6 mm i.d.) at ambient temperature. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed method was successfully applied for simultaneous determination of ondansetron and omeprazole in routine analysis.

Keywords

Simultaneous Estimation, RP-HPLC, Omeprazole, Ondansetron.

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