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Singh, Vibha
- Profile of HIV Positive Attendees of an Integrated Counseling and Testing Centre in Meerut- a Changing Trend
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Authors
Affiliations
1 Department of Microbiology Subharti Medical College, Swami Vivekananda Subharti University, Meerut, Uttar Pradesh
1 Department of Microbiology Subharti Medical College, Swami Vivekananda Subharti University, Meerut, Uttar Pradesh
Source
Indian Journal of Public Health Research & Development, Vol 4, No 2 (2013), Pagination: 44-47Abstract
Epidemiology of HIV-AIDS in an area especially with regards to socio-demographic factors helps in choosing and implementing a particular intervention in much better way. Study included counseling and testing of clients that were either self initiated or referred from the hospital over a period of 2 years. Analysis of ICTC data of all positive attendees was done. HIV positivity rate was 1% with male to female ratio of 2.2:1. Majority of positive males were literate and belonged to service class whereas most of the positive females were housewives. Spillage of the epidemic on to general population might well cause significant alteration in trends noted till times.Keywords
HIV, Seropositive, ICTC, Attendees, CounselingReferences
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- Joardar GK, Sarkar A, Chatterjee C, Bhattacharya RN, Sarkar S, Banerjee P. Profile of attendees in the voluntary counseling and testing centre of north Bengal Medical College in Darjeeling of West Bengal. Indian Journal of Community Medicine 2006; 31:237-240
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- Role of SLC40A1 R-178G Gene Mutation on Pathophysiology of Iron Deficient Sickle Cell Anemia Patients
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Materials & Methods: A total of 140 iron-deficient sickle syndrome patients were recruited for the study with an equal number for controls. The sickle cell sub-type was diagnosed by a cation exchange high performance liquid chromatography (HPLC) and complete blood count analysis was done by automated hematoanalyzer. Screening of iron deficiency was done by serum ferritin analysis using ELISA method. Genomic DNA was isolated from peripheral blood by kit method and DNA quantification was done by Nano drop analytical Software system. SLC40A1 R178G genotype analysis was performed by PCR-RFLP method using HpyAV restriction enzyme.
Results: Among the Sickle cell disease (SCD) patients, 17 were heterozygous and 09 were homozygous for SLC40A1(R178G) mutation; while 13 controls were heterozygous and 5 patients were homozygous for SLC40A1(R178G) mutation. We reported the significant elevation of serum ferritin and hemoglobin level in SLC40A1(R178G) mutation while a decrease in the ESR and CRP levels were observed.
Conclusion: The findings of the study suggested high impact of SLC40A1(R178G) mutation in pathophysiology of iron deficient SCD and shows positive correlation. It may act as the predictor of disease severity. Detection of this mutation in iron-deficient SCD patients is useful in treatment decision.
Based on this finding, clinicians can be more confident about iron status in SCD patients. Data from this research can be used to understand the status of iron overload in mutant versus non-mutant conditions. The Data of the study provides a genotype–phenotype correlation of SLC40A1 (R178G) mutation in iron-deficient SCD patients.
Authors
Sweta Pandey
1,
Shalini Kunder
2,
Naresh Bajaj
1,
Sudhakar Dwivedi
3,
Vibha Singh
4,
Sanjay Kumar Pandey
5
Affiliations
1 Department of Pediatrics, Shyam Shah Medical College, Rewa, MP, IN
2 Department of Zoology, Govt. Girl PG College, MP, IN
3 Department. of Anesthesiology, Shyam Shah Medical College, Rewa, MP, IN
4 Department of Biotechnology, Guru Ghasidas University, Bilashpur (CG), IN
5 Multidisciplinary Research Unit, Shyam Shah Medical College, Rewa, MP, IN
1 Department of Pediatrics, Shyam Shah Medical College, Rewa, MP, IN
2 Department of Zoology, Govt. Girl PG College, MP, IN
3 Department. of Anesthesiology, Shyam Shah Medical College, Rewa, MP, IN
4 Department of Biotechnology, Guru Ghasidas University, Bilashpur (CG), IN
5 Multidisciplinary Research Unit, Shyam Shah Medical College, Rewa, MP, IN
Source
The Indian Practitioner, Vol 74, No 12 (2021), Pagination: 18-22Abstract
Introduction: Solute carrier family 40 member 1(SLC40A1) is a protein coding gene. The protein encoded by this gene is a cell membrane protein that plays a key role in the majority of iron transportation by balancing cellular and systemic iron levels. Defects in this gene are a cause of hemochromatosis type 4 (HFE4) and Hemochromatosis type 1 diseases. Ironoverload and a non-responsive phenotype are also associated with the hereditary variants of iron deficiency anemia. Based on this, it was proposed that the presence of this mutation could influence iron absorption and provide protection against the severity of iron deficiency in sickle syndrome. SLC40A1 gene mutations may have a useful clinical result in the severity of the disease due to their diverse role in iron metabolism.Materials & Methods: A total of 140 iron-deficient sickle syndrome patients were recruited for the study with an equal number for controls. The sickle cell sub-type was diagnosed by a cation exchange high performance liquid chromatography (HPLC) and complete blood count analysis was done by automated hematoanalyzer. Screening of iron deficiency was done by serum ferritin analysis using ELISA method. Genomic DNA was isolated from peripheral blood by kit method and DNA quantification was done by Nano drop analytical Software system. SLC40A1 R178G genotype analysis was performed by PCR-RFLP method using HpyAV restriction enzyme.
Results: Among the Sickle cell disease (SCD) patients, 17 were heterozygous and 09 were homozygous for SLC40A1(R178G) mutation; while 13 controls were heterozygous and 5 patients were homozygous for SLC40A1(R178G) mutation. We reported the significant elevation of serum ferritin and hemoglobin level in SLC40A1(R178G) mutation while a decrease in the ESR and CRP levels were observed.
Conclusion: The findings of the study suggested high impact of SLC40A1(R178G) mutation in pathophysiology of iron deficient SCD and shows positive correlation. It may act as the predictor of disease severity. Detection of this mutation in iron-deficient SCD patients is useful in treatment decision.
Based on this finding, clinicians can be more confident about iron status in SCD patients. Data from this research can be used to understand the status of iron overload in mutant versus non-mutant conditions. The Data of the study provides a genotype–phenotype correlation of SLC40A1 (R178G) mutation in iron-deficient SCD patients.
Keywords
PCR, RFLP, SCD, IDA, SLC40A1, SNPReferences
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