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Molecular Profiling of Dendrocalamus asper and D. Hamiltonii Using RAPD, ISSR and SSR Markers - Effects of DNA Extraction Methods on PCR Amplification


Affiliations
1 Department of Biotechnology, Kurukshetra University, Kurukshetra-136119
2 Centre for Plant Biotechnology, CCS HAU Campus, Hisar-125004, India
3 C-en tral University of Punjab,B hatinda-151001, (Punjab), India
     

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Extraction of good quality DNA is the most essential requirement for molecular analysis of any species. Five protocols for DNA extraction were compared to assess their effects on amount of DNA recovered, its purity and efficacy in PCR amplification using RAPD, ISSR and SSR markers in two species of Dendrocalamus asper. and D. hamiltonii. Leaf tissues from in vitro cultures, tissue culture raised plants in green house,and young and mature leaves from field grown plants of the two species were subjected to five DNA extraction methods using the protocols. Significant differences in DNA quantity and quality between samples and protocols were observed. Leaves of tissue culture raised plants growing in the green house gave best DNA yield (5681, 5015 ng/ μl)followed by in vitro shoot cultures (4645, 4379 ng/ μl), and young (2720, 1801 ng/ μl) and mature leaves (1800, 1368 ng/ μl) from field grown plants of D. hamiltonii and D. asper respectively.The concentration of DNA obtained was more for D. hamiltonii samples than the corresponding D. asper tissues.

Keywords

Bamboo, Dendrocalamus, DNA Extraction Methods, PCR Efficiency, Quality and Quantity of DNA
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About The Authors

Sharbati R. Singh
Department of Biotechnology, Kurukshetra University, Kurukshetra-136119

Sunita Dalal
Department of Biotechnology, Kurukshetra University, Kurukshetra-136119

Rohtas Singh
Centre for Plant Biotechnology, CCS HAU Campus, Hisar-125004
India

A .K. Dhawan
C-en tral University of Punjab,B hatinda-151001, (Punjab)
India

Rajwant K. Kalia


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  • Molecular Profiling of Dendrocalamus asper and D. Hamiltonii Using RAPD, ISSR and SSR Markers - Effects of DNA Extraction Methods on PCR Amplification

Abstract Views: 544  |  PDF Views: 3

Authors

Sharbati R. Singh
Department of Biotechnology, Kurukshetra University, Kurukshetra-136119
Sunita Dalal
Department of Biotechnology, Kurukshetra University, Kurukshetra-136119
Rohtas Singh
Centre for Plant Biotechnology, CCS HAU Campus, Hisar-125004, India
A .K. Dhawan
C-en tral University of Punjab,B hatinda-151001, (Punjab), India

Abstract


Extraction of good quality DNA is the most essential requirement for molecular analysis of any species. Five protocols for DNA extraction were compared to assess their effects on amount of DNA recovered, its purity and efficacy in PCR amplification using RAPD, ISSR and SSR markers in two species of Dendrocalamus asper. and D. hamiltonii. Leaf tissues from in vitro cultures, tissue culture raised plants in green house,and young and mature leaves from field grown plants of the two species were subjected to five DNA extraction methods using the protocols. Significant differences in DNA quantity and quality between samples and protocols were observed. Leaves of tissue culture raised plants growing in the green house gave best DNA yield (5681, 5015 ng/ μl)followed by in vitro shoot cultures (4645, 4379 ng/ μl), and young (2720, 1801 ng/ μl) and mature leaves (1800, 1368 ng/ μl) from field grown plants of D. hamiltonii and D. asper respectively.The concentration of DNA obtained was more for D. hamiltonii samples than the corresponding D. asper tissues.

Keywords


Bamboo, Dendrocalamus, DNA Extraction Methods, PCR Efficiency, Quality and Quantity of DNA

References