Refine your search
Collections
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Karunakaran, M.
- Studies on the Effects of Ph and Incubation Period on Protease Production by bacillus spp. Using Groundnut Cake and Wheat Bran
Abstract Views :442 |
PDF Views:114
Authors
Affiliations
1 Department of Industrial Biotechnology, Bharath University, Chennai-600 073, IN
2 Chemical Engineering Department, Anna University, Chennai-600 025, IN
3 Department of Biotechnology, St. Peter’s Engineering College, Chennai-54, IN
1 Department of Industrial Biotechnology, Bharath University, Chennai-600 073, IN
2 Chemical Engineering Department, Anna University, Chennai-600 025, IN
3 Department of Biotechnology, St. Peter’s Engineering College, Chennai-54, IN
Source
Indian Journal of Science and Technology, Vol 1, No 4 (2008), Pagination: 1-4Abstract
The production of alkaline protease by Bacillus licheniformis (MTCC 1483) and Bacillus subtilis of indigenous isolates using cheapest sources- groundnut cake (N2 Source) and wheat bran (C source) was studied. The proteolytic activity was found maximum at 72 hr of the culture for both the bacterial types but the pH of the medium for maximal enzyme production varied as 9 for Bacillus licheniformis and as 8 for Bacillus subtilis. Thus investigations of this study reveal the possibility of employing cheapest nutrient source for microbial proteases that can be used on a commercial scale.Keywords
pH, Protease, Bacillus licheniformis, B. subtilis, Groundnut Cake, Wheat BranFull Text
References
- Abu Sayem SM, Alam MJ and Mozammel Hoq MD (2006) Effect of temperature, pH and metal ions on the activity and stability of alkaline protease form novel Bacillus licheniformis MZK03. Proc. Pakistan Acad. Sci. 43(4), 257-262.
- Anson M.L (1938). The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J. Gen. Physiol. 22, 79-89.
- Aslim B, Yuksekdag ZN, Beytali Y (2002) Determination of PHB growth quantities of certain Bacillus species isolated from the soil. Turkish Electronic J. Biotechnol. 24- 32.
- Beg KB, Gupta R (2003) Purification and characterization of an oxidation-stable, thiol-dependent serine alkaline protease form Bacillus mojavensis, Enz. Microbial Technol. 32, 294-304.
- Beg KB, Sahai V, Gupta R (2003) Statistical media optimization and alkaline protease production form Bacillus mojavensis in a bioreactor. Process Biochem. 39,2003- 2009.
- Dixit VS and Pant A (2000) Hydrocarbon degradation and protease production by Nocardiopsis sp. NCIM 5124. Letters in Applied Microbiol. 30:67–69.
- Ellaiah P, Adinarayana K, Rajyalaxmi P, Srinivasulu B (2003) Optimization of process parameters for alkaline protease production under solid state fermentation by alkalophilic Bacillus sp. Asian J. Microbial Biotechnol. Environ. Sc. 5,49-54.
- Emmanuel A. Abu and Saleh A. Ado (2004). Comparative studies on the effect of organic and inorganic nitrogen supplementation of millet and sorghum pomace on the production of three industrial enzymes by Aspergillus niger SL.1. Biokemistri. 16, 64-70.
- Fikret Uyar and Zubeyde Baysal (2004) Production and Optimization of process parameters for alkaline protease production by newly isolated Bacillus sp. under solid state fermentation. Process Biochem. 39,1893-1898.
- Folasade M. Olajuyigbe and Joshua O. Ajele (2005) Production dynamics of extracellular protease from Bacillus species. African J. Biotechnol. 4(8), 776- 779.
- Folin O & Ciocalteu V (1927). On tyrosine and tryptophan determination in proteins. J. Biol. Chem. 73, 627-649.
- Han-Seung Joo and Chung-Soon Chang (2005) Production of protease from a new alkalophilic Bacillus sp. I-312 grown on soybean meal: Optimization and some properties. Process Biochem. 40,1263- 1270.
- Krishna Suresh Babu Naidu and Kodhidela Lakshmi Devi (2005) Optimization of thermostable alkaline protease production from species of Bacillus using Groundnut cake. African J. Biotechnol. 4 (7), 724- 726.
- Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951) Protein measurement with Folin Phenol Reagent. J.Biol. Chem. 193, 265-275.
- Mabrouk SS, Hashem AM, El-Shayeb NMA, Ismail AMS and Abdel-Fattah AF (1999) Optimization of alkaline protease productivity by Bacillus licheniformis ATCC 21415. Bioresource Technol. 69,155-159.
- Mona K. Gouda (2006) Optimization and purification of alkaline proteases produced by Marine Bacillus sp. MIG newly isolated from eastern harbor of Alexandria. Polish J. Microbiol. 55, 119-126.
- Nascimento WCA, Martins MLL (2004). Production and properties of an extracellular protease from thermophilic Bacillus sp. Braz. J. Microbiol. 35, 1-2.
- Pastor MD, Lorda GS, Balatti A (2001) Protease obtention using Bacillus subtilis 3411 and amaranth seed meal medium at different aeration rates. Braz. J. Microbiol. 32, 1-8.
- Wellingta Cristina Almeida do Nascimento and Meire Lelis Leal Martins (2006) Studies on the stability of protease from Bacillus sp. and its compatibility with commercial detergent. Brazilian J. Microbiol. 37, 307-311.
- Wellingta Cristina Almeida do Nascimento and Meire Lelis Leal Martins (2004) Production and properties of an extracellular protease from thermophilic Bacillus sp. Brazilian J. Microbiol. 35, 91- 96.