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Obembe, Olawole O.
- Irregular Deposition of Cell Wall Polymers Resulting from Defective Cellulose Synthase Complexes
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1 Laboratory of Plant Breeding, Wageningen University, Box 386 6700AJ Wageningen, NL
1 Laboratory of Plant Breeding, Wageningen University, Box 386 6700AJ Wageningen, NL
Source
Indian Journal of Science and Technology, Vol 2, No 3 (2009), Pagination: 5-8Abstract
The crystalline cellulose microfibril is formed by the spontaneous association of about 36 β-D-glucan chains, which are simultaneously synthesised by a large membrane-localised multi-enzyme cellulose synthase complex. Antisense technology has been previously employed separately on two of the constituent cellulose synthase catalytic subunits (CesA) of the complex in potato (Solanum tuberosum), namely CesA2 and CesA4, to generate potato tuber cell walls with reduced cellulose content. Genetic crossing of two transgenic potato lines csr2-1 and csr4-8 was carried out to investigate the effects of two defective CesAs in the same genetic background, with respect to cellulose deposition in the potato tuber cell walls. It was striking to observe, through fluorescence microscopy with calcofluor white, a strong fluorescence in the cell corners and less prominent and uneven fluorescence around the cells of the csr2 tubers as compared to others. It was also noted that these phenotypes were not pronounced in the csr2/csr4 double transformants as expected.Keywords
Cell Wall, Cellulose Synthase, Polysaccharide Deposition,Solanum tuberosumFull Text
References
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- Recent Advances in Plastid Transformation
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Authors
Affiliations
1 Department of Biological Sciences, Covenant University, PMB 1023 Ota, Ogun State, NG
2 Plant Transformation Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, IN
1 Department of Biological Sciences, Covenant University, PMB 1023 Ota, Ogun State, NG
2 Plant Transformation Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, IN
Source
Indian Journal of Science and Technology, Vol 3, No 12 (2010), Pagination: 1229-1235Abstract
Plastid transformation offers a viable alternative to nuclear transformation because of its numerous advantages. It was against this backdrop that various groups of researchers have been exploiting this group of sub-cellular organelles, over the last two decades, for the genetic engineering of agronomic traits and metabolic pathways, as well as for molecular farming for the production of plant-derived high-valued biopharmaceuticals and industrial proteins. In this short review, we discuss the evolution and development of this technology with respect to the current state-of-the-art, which places it in high pedestal as a cost effective and safe production technology for high quality molecular farming products as well as a highly efficient method to create new metabolic pathways and improve the agronomic traits.Keywords
Chloroplast Transformation, Molecular Farming, Metabolic Engineering, Agronomic Trait EngineeringFull Text
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