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Ebenezer, P.
- Optimization of Extracellular Lipase Production in Colletotrichum Gloeosporioides by Solid State Fermentation
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Authors
V. Balaji
1,
P. Ebenezer
1
Affiliations
1 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai-600 025, Tamil Nadu, IN
1 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai-600 025, Tamil Nadu, IN
Source
Indian Journal of Science and Technology, Vol 1, No 7 (2008), Pagination: 1-8Abstract
Twenty two endophytic fungi isolated from oil seeds were screened for their ability to produce extracellular lipolytic enzymes in solid state fermentation. Among them, the most productive strain, identified as Colletotrichum gloeosporioides was cultivated for lipase production in solid state fermentation (SSF) using oil-mill residue as a solid support. The elaboration of extracellular lipases from C. gloeosporioides on different residual cheap oil substrates was tested. Among these, pongamia oil cake (POC) showed maximum lipase activity (983 U/g dry matter (DM)) followed by coconut oil cake (COC) (925 U/g DM). These two oil industrial residues were chosen for enhanced lipase production. The maximum enzyme yield was obtained when POC was impregnated with CDB (Czapek-Dox Broth) in the ratio of 1:1.5 and with (906 U/g DM). Triton X 100 as the extractant from the fermented solids yielded 1020 U/g DM. The optimum temperature for lipase secretion by C. gloeosporioides was found to be 25°C. Xylose as the carbon source and peptone as the nitrogen source were favorable for the secretion of the enzymes. Tween 60 served as a good lipid substrate for lipase production (1170U/g DM) when POC was the substrate. Amending the POC with Magnesium sulphate as a metallic ion source induced good lipase activity (1240 U/g DM) by C. gloeosporioides. The Sunflower oil was found to be best co-vegetable oil substrate to induce the enzyme (2560 U/g DM). Triton X100 served as the surfactant for lipase secretion in C. gloeosporioides. Thus, pongamia oil cake can be used as cheap substrate for enhanced extracellular lipase production by C. gloeosporioides.Keywords
Lipases, Colletotrichum gloeosporioides, Solid State Fermentation, Pongamia Oil Cake, Coconut Oil CakeFull Text
References
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- A New Report on Mycobiota Associated with Ropalidia marginata Paper Nests
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PDF Views:109
Authors
Affiliations
1 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai-600 025, Tamil Nadu, IN
1 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai-600 025, Tamil Nadu, IN
Source
Indian Journal of Science and Technology, Vol 3, No 1 (2010), Pagination: 6-8Abstract
Twenty four fungal species were isolated from the paper nests of common Indian paper wasp Ropalidia marginata. The genus Aspergillus was dominant while Penicillium recorded next in the paper nests. Among Aspergillus spp., A. niger was most abundant (14%), followed by A. flavus (7%). Among Penicillium spp., P. citrinum occurred high (9%) followed by P. oxalicum (2%). The nest material analysed by GC-MS revealed that it was predominantly wax and that the components were mainly hydrocarbons.Keywords
Mycoflora, Aspergillus, Paper Nests, Penicillium, Ropalidia marginata, Wasp, New ReportFull Text
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Abstract Views :593 |
PDF Views:174
Authors
Affiliations
1 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, IN
1 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, IN
Source
Indian Journal of Science and Technology, Vol 3, No 2 (2010), Pagination: 113-117Abstract
Aspergillus japonicus isolated from the paper nest of Ropalidia marginata was screened for its extracellular lipase production. Maximum lipase activity (120 U/ml) was achieved by manipulating the culture environment. The optimum pH was 7.5 and the temperature was 40°C. Sucrose served as the best carbon source whereas peptone was the best nitrogen source for lipase production. An animal fat, pig fat in the culture medium enhanced lipase production. These investigations will be helpful in further scaling-up processes of this industrial enzyme. Owing to many applications in high-value syntheses and as bulk enzymes, lipases have high impact on bioprocessing.Keywords
Aspergillus japonicus, Paper Nest, Pig Fat, Ropalidia marginata, Lipase, Tween-20Full Text
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