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Karthickeyan, S. M. K.
- Molecular Typing and Mapping of MHC Class II-DRB3 Gene in Indian River Buffaloes (Bubalus bubalis)
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Authors
Affiliations
1 Dept. of Animal Genetics and Breeding, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Chennai - 600 007, IN
1 Dept. of Animal Genetics and Breeding, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Chennai - 600 007, IN
Source
Indian Journal of Science and Technology, Vol 3, No 5 (2010), Pagination: 557-560Abstract
The present study was undertaken with the objectives of characterizing Bubu-MHC loci by PCR and genotyping MHC loci for allelic variation. The PCR product of second exon of the Bubu-MHC-DRB3 gene (304 bp) exhibited genetic polymorphism while digesting with HaeIII enzyme resulting in three restriction fragment patterns in Murrah, four in Surti and three in Murrah graded buffaloes. In all the three genetic groups, the pattern `b' (82, 222 bp) was frequently observed. The restriction fragment analysis with RsaI revealed five patterns in Murrah and three in Surti. The pattern `s' (67, 93, 144 bp) with a frequency of 0.4444 and `l' (67, 237 bp) with a frequency of 0.5000 were observed. Microsatellite typing revealed nine alleles ranging from 160 to 212 bp at the second intron. In Murrah, the allele 190 bp was observed exclusively. In Surti, alleles 192 and 212 bp were observed more frequently. RsaI enzyme revealed more polymorphic patterns of DRB3 than HaeIII. Microsatellite typing provided certain breed-specific alleles. This gene was physically localized to chromosome 2p following tyramide signal amplification in between bands 15-22 using (cDNA probes derived from Bos taurus cattle) fluorescence in situ hybridization.Keywords
Bubu-MHC, PCR-RFLP, FISH, BuffaloesFull Text
References
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- Microsatellite Analysis of Kangayam Cattle (Bos indicus) of Tamilnadu
Abstract Views :350 |
PDF Views:60
Authors
Affiliations
1 Dept. of Animal Genetics & Breeding, Madras Veterinary College, Chennai – 600 007, IN
1 Dept. of Animal Genetics & Breeding, Madras Veterinary College, Chennai – 600 007, IN
Source
Indian Journal of Science and Technology, Vol 2, No 10 (2009), Pagination: 38-40Abstract
Assessment of genetic variability in Kangayam breed of cattle in Tamilnadu, South India was carried out using 25 bovine microsatellite markers. The mean number of alleles was 4.04 ± 0.09 with a range of 2 to 6 and the allele size ranged from 94 to 300 bp. The frequency distribution of alleles in the breed was from 0.0104 to 0.9167. The estimated heterozygosity was 0.6183 ± 0.01 and the PIC was 0.5628 ± 0.03. The overall mean within-population inbreeding estimate (FIS) value (- 0.084) suggested excess of heterozygotes in the population. In addition, higher PIC value indicated the scope for maintaining variation in the population and strategies to take meaningful conservation.Keywords
Cattle, Kangayam, Microsatellites, PICFull Text
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- Naveen Kumar S, Jayashankar MR, Nagaraja CS, Govindaiah MG, Saravanan R and Karthickeyan SMK (2006) Molecular characterization of Hallikar breed of cattle using microsatellite markers. Asian- Aust. J. Anim. Sci. 19, 622–626.
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- Assessment of with-in Breed Diversity in Hallikar Cattle (Bos indicus) through Microsatellite Markers
Abstract Views :471 |
PDF Views:118
Authors
Affiliations
1 Department of Biotechnology, Vel Sri Ranga Sanku College (Arts & Science), Chennai-600 062, IN
2 Department of Animal Genetics & Breeding, Madras Veterinary College, Chennai-600 007, IN
1 Department of Biotechnology, Vel Sri Ranga Sanku College (Arts & Science), Chennai-600 062, IN
2 Department of Animal Genetics & Breeding, Madras Veterinary College, Chennai-600 007, IN
Source
Indian Journal of Science and Technology, Vol 4, No 8 (2011), Pagination: 895-898Abstract
Hallikar cattle, a popular draft breed of India, were assessed genetically using five microsatellite markers, as recommended by FAO. All the screened loci were polymorphic and a total of 39 alleles were observed across the analyzed loci. The mean number of alleles was found to be 7.8±1.92 with a range of 5 to 10. The allele size ranged from 122 to 302 bp. The frequency distribution of microsatellite alleles in the breed was from 0.0104 to 0.7138. The estimated expected heterozygosity value was 0.6896±0.1403 and the PIC was 0.6565±0.1378. The population was not in Hardy-Weinberg equilibrium proportion due to systematic and dispersive processes acting upon. The within population inbreeding estimate was 0.1331, indicating deficiency of heterozygosity considerably in population of Hallikar cattle. The panel of microsatellites used was highly informative for molecular characterization and could be used for exploitation of genetic diversity of the related breeds for conservation. No mode shift was detected in the frequency distribution of alleles and a normal L-shaped curve was observed, indicating that the population is nonbottlenecked and stable with respect to population size.Keywords
Hallikar Cattle, Heterozygosity, Microsatellites, PICFull Text
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- Karthickeyan SMK, Saravanan R and Thangaraju P (2006) Characterization of Krishna Valley breed of cattle (Bos indicus) in south India using microsatellite markers. Livestock Res. for Rural Devpt. 8 (11), 1-6.
- Karthickeyan SMK, Sivaselvam SN, Selvam R and Thangaraju P (2009) Microsatellite analysis of Kangayam cattle (Bos indicus) of Tamilnadu. Indian J. Sci. Technol. 2 (10), 38-40. Domain site: http://www.indjst.org.
- Karthickeyan SMK, Sivaselvam SN, Selvam R, Raja TV, Rajendran R and Thangaraju P (2007) Umblachery breed of cattle in south India: Genetic assessment through microsatellite markers. Asian J. Anim. Vet. Adv. 2(4), 218-222.
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