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Jalalpoor, Shila
- Prevalence of Nanostructure of Surface Layer among the Bacteria Isolated from Environment
Abstract Views :341 |
PDF Views:106
Authors
Affiliations
1 Lecture of Microbiology, Shahreza Branch, Islamic Azad University, and Membership of young researchers club, IR
1 Lecture of Microbiology, Shahreza Branch, Islamic Azad University, and Membership of young researchers club, IR
Source
Indian Journal of Science and Technology, Vol 5, No S3 (2012), Pagination: 2417-2467Abstract
Nanostructure of S-layer forms the monomolecular outermost protein layer in bacteria and archaea that credited with protein or glycoprotein subunits and has crystalline biopolymer structure. This layer protects bacteria to phagocytosis and prohibits the entry of some molecules e.g. antibiotics. The premises of hospital environment can serve as reservoirs of potential pathogens. The goal of study to was investigate the Nanostructure of Surface layer among the Bacillus cereus isolates from hospital environment. The research was performed with laboratory method during 2005- 2007 from Azzahra Hospital and Esfahan University (Isfahan province of Iran). Totally, 194 samples were collected from hospital surface. Environmental samples collected with swab in Nutrient Broth (NB). Bacterial identification was performed based on Bacteriological standard methods using selective culture medium. Samples, cultured in TSA for 16 h, in aerobic condition were then separated for surface proteins and subjected to electrophoresis along with molecular weight marker. S-Layer in B. cereus has 97 KD of molecular weight. From 194 bacterial isolates among hospital environment, the frequency of Bacillus sp. was 26.29%. Among 13 B. cereus strains from hospital environment, only one strain (7.69%) was the producer of S-layer. Result showed that the prevalence B. cereus strain with S-layer in hospital sensitive environment is due to increased antibiotic resistance for nosocomial infection and it is necessary to continue the reduction of transfer of virulence agent and antibiotic resistant in pathogen bacteria.Keywords
Nanostructure of Surface Layer, Bacillus cereus, Antibiotic Resistant, Hospital EnvironmentFull Text
References
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- Hilliard NJ, Schelonka RL and Waites KB (2003) Bacillus cereus bacteremia in a preterm neonate. J. Clin. Microbiol. 41, 3441-3444.
- Jalalpoor S, Kasra Kermanshahi R , Nouhi AS and Zarkesh H (2010b) Survey Frequence of ß-lactamase Enzyme and Antibiotic Sensitivity Pattern in Isolated Pathogen Bacteria from Low and High Hospital Contact Surfaces. Pajuhandeh J. 15(2), 77-82.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Esfahani (2009d) Antibiotic Resistance in B.cereus st. Isolated from Staff Hands and Hospital Surfaces. Third Iranian Congress of Clinical Microbiology, Iran.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Esfahani H (2009c) The comparative frequency the ß-lactamase Production and antibiotic susceptibility pattern of Bacterial stains isolated from Staff Hands and Hospital Surfaces in Azzahra Hospital–Isfahan. Iran. J. Med. Microbiol. 3(4), 37-45.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Esfahani H (2010a) Survey Characterization Nano Structure Surface Layer in Some of Pathogen Bacteria. Zahedan J. Res. Med. Sci. 23(4), 470-477.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Isfahani H (2009b) Survey effect of in-vivo and in-vitro condition on expression of surface layer genes in bacteria. J. Iran. Chem. Soc. 6 (Suppl), S11.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Isfahani H (2009a) The Prevalence of Nanostructure Surface Layer in Bacillus Cereus Strains Isolated from Staff Hands and Hospital Surfaces. J. Isfahan Med. School, 27(100), 632-645.
- Jalalpoor S, Kermanshahi KR, Nouhi A and Zarkesh H (2007) Study of ß-lactamase and S-layer Production in some of Isolated Pathogen Bacteria From Clinical and Environmental Hospital Samples. MSc thesis,Iran, Tehran, Islamic Azad University Science and Research Branch Tehran, Iran.
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- Kotiranta AK, Hitoshi I, Markus P, Haapasalo P and Kari L (1999) Radiation sensitivity of Bacillus cereus with and without a crystalline surface protein layer. FEMS Microbiol. Lett.179, 275-280.
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- Masahiro Y, Hirofuji T, Motooka N, Nozoe K, Shigenaga K and Anan H (2003) Humoral Immune Responses to S-layer-Like Proteins of Bacteroides forsythus. Clin. Diagnost. Laborate. Immune.10(3), 383–387.
- Mesnage S, Haustant M and Foue A (2001) A general strategy for identification of S-layer genes in the Bacillus cereus group :molecular characterization of such a gene in Bacillus thuringiensis subsp. galleriae NRRL 4045.J Microb.147,1343-51.
- Messner P, Steiner K, Zarschler K and Schaffer C (2008) S-layer nanoglycobiology of bacteria. Carbohydr. Res. 343(12),1934-51.
- Mielke M (2010) Prevention and control of nosocomial infections and resistance to antibiotics in Europe - Primum non-nocere: elements of successful prevention and control of healthcare-associated infections. Int. J. Med. Microbiol.300(6), 346-50.
- Sambrook J and Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor,NY: Cold Spring Harbor Laboratory Press.
- Sara M (2001) Conserved anchoring mechanisms between crystalline cell surface S-layer proteins and secondary cell wall polymers in Gram-positive bacteria. Trends Microbiol. 9, 47–9.
- Sara M and Uwe B (2000) Sleytr.S-layer Proteins. J Bacteriol.182(4), 859–68.
- Schaffer C and Messner P (2001) Glycobiology of surface layer proteins. Biochimie.83, 591-99.
- Schaffer C and Paul M (2005) The structure of secondary cell wall polymers: how Gram-positive bacteria stick their cell walls together. Microbiol.15, 643–651.
- Sehulster L and Raymond YW (2003) Guidelines for Environmental Infection Control in Health-Care Facilities. U.S. Department of Health and Human Services Centers for Disease Control and Prevention (CDC).Atlanta GA 30333.
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- Vander Zwet WC, Parlevliet GA, Savelkoul PH, Stoof J and Kaiser AM (2003) Outbreak of Bacillus cereus infection in a neonatal intensive care unit traced to balloons used in manual ventilation. J. Clin. Microbiol. 38, 4131-6.
- Washington C, Stephen A, Janda W , Koneman E, Procop G, Schreckenberger P and Woods G (2006) Koneman's Color Atlas and Textbook of Diagnostic Microbiology, Sixth edition.USA: Lippincott wiiliams & wilkins, pp: 775-9.
- Spread of Nanostructure of S-layer among the Bacteria Isolated from Skin
Abstract Views :332 |
PDF Views:102
Authors
Affiliations
1 Lecture of Microbiology, Shahreza Branch, Islamic Azad University, and Membership of Young Researchers Club, IR
1 Lecture of Microbiology, Shahreza Branch, Islamic Azad University, and Membership of Young Researchers Club, IR
Source
Indian Journal of Science and Technology, Vol 5, No S3 (2012), Pagination: 2421-2424Abstract
An S-layer (surface layer) is a part of the cell envelope commonly found in bacteria, as well as among archaea. It consists of a monomolecular layer composed of identical proteins or glycoproteins. As for many bacteria, the S-layer represents the outermost interaction zone with their respective environment, its functions are very diverse and vary from species to species; subject of this study was to find out the prevalence of Nanostructure of Surface layer among the Bacillus cereus isolated from the skin of clinical staff. Relevant information to Surface Layer was extracted from articles that listed in Pubmed, Elsivier Science, and Yahoo from 1995 to 2010 years. The research was performed with laboratory method during 2005-2007 years in Azzahra Hospital and Esfahan University. Bacterial identification was performed with standard bacteriological methods and selective culture medium was applied in preparation of samples. Bacterial samples were cultured in TSA, for 16 hour, in aerobic condition then separated the surface proteins to run electrophoresis with molecular weight marker. S-Layer in Bacillus cereus has 97KD molecular weight. Among the 13 Bacillus cereus strains isolated from skin of staff, 11 (84.60%) showed positive for S-layer. S-layers in pathogenic bacteria offer protection against bacteriophages and phagocytosis, resistance against low pH ,adhesion, stabilisation of the membrane and provide adhesion sites for exoproteins due to more pathogenesis, infection resistant and antibiotic resistant to bacteria. Result of this study shows the prevalence of S-layer in pathogen bacteria and as important to determine S-layer producer strains in laboratory.Keywords
Surface Layer, Pathogen Bacteria, Bacillus cereusFull Text
References
- Eichler I (2003) Facing extremes: archaeal surfacelayer (glyco) proteins. Microbiol. 149, 3347-3351.
- Hilliard NJ, Schelonka RL and Waites KB (2003) Bacillus cereus bacteremia in a preterm neonate. J. Clin. Microbiol. 41, 3441-3444.
- Jalalpoor S, Kasra Kermanshahi R, Noohi A and Zarkesh Esfahani H (2007) Study of ß-lactamase and S-layer Production in some of Isolated Pathogen Bacteria From Clinical and Environmental Hospital Samples. M.Sc. thesis, Iran, Tehran, Islamic Azad University Science and Research Branch Tehran, Iran.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Esfahani H (2009) Survey and Comparative Bacterial Spread Pattern in Staff Hands and High and Low Contact Hospital Surfaces. Third Iranian Congress of Clinical Microbiology, Shiraz, Iran.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Esfahani H (2009a) The comparative frequency the ß-lactamase Production and antibiotic susceptibility pattern of bacterial stains isolated from Staff Hands and Hospital Surfaces in Azzahra Hospital–Isfahan. Iran J. Med. Microbiol. 3(4), 37-45.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Esfahani H (2009b) Antibiotic Resistance in B.cereus st. Isolated from Staff Hands and Hospital Surfaces. Third Iranian Congress of Clinical Microbiology, Shiraz, Iran.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Esfahani H (2010a) Survey Characterization Nano Structure Surface Layer in Some of Pathogen Bacteria. Zahedan J. Res. Med. Sci. 12(4): In Press.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Isfahani H (2009c) The Prevalence of Nanostructure Surface Layer in Bacillus cereus strains Isolated from Staff Hands and Hospital Surfaces. J. Isfahan Med. Sch. 27(100), 632-645.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS and Zarkesh Isfahani H (2009d) Survey effect of in-vivo and in-vitro condition on expression of surface layer genes in bacteria. J. Iran Chem. Soc. 6 (Suppl): S11.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS, Zarkesh Esfahani H and Mobasherizadeh S (2010b) Survey Prevalence and Resistance to Some Beta lactame Antibiotics in Bacillus cereus strains Isolated of AZZAHRA Hospital. Iran J. Biol. 23(4), 470-477.
- Jalalpoor Sh, Kasra Kermanshahi R , Nouhi A.S , Zarkesh Esfahani H (2008) Study to Spreading Bacteria in How and Low Contact Surfaces in Hospital. 9th Iranian Congress of Microbiology, Iran, kerman.4-6 March.208.
- Jalalpoor Sh,Kasra Kermanshahi R , Nouhi A.S , Zarkesh Esfahani H (2010) Survey Frequency of ß- lactamase Enzyme and Antibiotic Sensitivity Pattern in Isolated Pathogen Bacteria from Low and High Hospital Contact Surfaces. Pajuhandeh J.15 (2), 77- 82.
- Kamp G and Kramer A (2004) Epidemiologic Background of Hand Hygiene and Evaluation of the Most Important Agents for Scrubs and Rubs. Clin. Microbiol. Rev. 17(4), 863-93.
- Kotiranta A, Haapasalo M and Kari K (1998) Surface Structure, Hydrophobicity, Phagocytosis, and Adherence to Matrix Proteins of Bacillus cereus Cells with and without the Crystalline Surface Protein Layer. Infect Immun. 66(10), 4895–4902.
- Kotiranta AK, Hitoshi I, Markus P, Haapasalo P and Kari L (1999) Radiation sensitivity of Bacillus cereus with and without a crystalline surface protein layer. FEMS Microbiol. Lett.179, 275-280.
- Masahiro Y, Hirofuji T, Motooka N, Nozoe K, Shigenaga K and Anan H (2003) Humoral Immune Responses to S-layer-Like Proteins of Bacteroides forsythus. Clin. Diagnost. Laborate. Immune. 10(3), 383–387.
- resistance to antibiotics in Europe - Primum nonnocere Mesnage S, Haustant M and Foue A (2001) A general strategy for identification of S-layer genes in the Bacillus cereus group: molecular characterization of such a gene in Bacillus thuringiensis subsp. galleriae NRRL 4045.J Microb.147, 1343-1351.
- Messner P, Steiner K, Zarschler K and Schaffer C (2008) S-layer nanoglycobiology of bacteria. Carbohydr. Res. 343(12),1934-1951.
- Mielke M (2010) Prevention and control of nosocomial infections and: elements of successful prevention and control of healthcare-associated infections. Int. J. Med. Microbiol. 300(6), 346-50.
- Rosenthal VD, Maki DG and Jamulitrat S (2010) International Nosocomial Infection Control Consortium (INICC) report, data summary for 2003– 2008. Am. J. Infect. Control. 38(2), 95–104.
- Rosenthal VD, Maki G and Jamulitrat DS (2010) International Nosocomial Infection Control Consortium (INICC) report, data summary for 2003- 2008, issued June 2009. Am. J. Infect. Control, 38, 95-106.
- Sambrook J and Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
- Sara M (2001) Conserved anchoring mechanisms between crystalline cell surface S-layer proteins and secondary cell wall polymers in Gram-positive bacteria. Trends Microbiol. 9, 47–9.
- Sara M and Sleytr UB (2000) S-layer Proteins. J. Bacteriol. 182(4), 859–68.
- Schaffer C and Messner P (2001) Glycobiology of surface layer proteins. Biochimie. 83, 591-99.
- Schaffer C and Paul M (2005) The structure of secondary cell wall polymers: how Gram-positive bacteria stick their cell walls together. Microbiol. 15, 643–651.
- Sehulster L and Raymond YW (2003) Guidelines for Environmental Infection Control in Health-Care Facilities. U.S. Department of Health and Human Services Centers for Disease Control and Prevention (CDC). Atlanta GA 30333.
- Todor k (2005) Bacterial Structural in Relationship to Pathogenicity, available at www.Texbook of Bacteriology.net.
- Vander Zwet WC, Parlevliet GA, Savelkoul PH, Stoof J,Kaiser AM (2000) Outbreak of Bacillus cereus infection in a neonatal intensive care unit traced to balloons used in manual ventilation. J. Clin. Microbiol .38, 4131-6.
- Washington C, Stephen A, Janda W , Koneman E, Procop G, Schreckenberger P and Woods G (2006) Koneman's Color Atlas and Textbook of Diagnostic Microbiology, Sixth edition.USA: Lippincott wiiliams & wilkins, pp:775-779.
- Survey the Role of in Vivo and in vitro Condition to Expiration of Nano Structure Para Crystalline Layer Gene in Bacteria
Abstract Views :341 |
PDF Views:100
Authors
Affiliations
1 Lecture of Microbiology, Shahreza Branch, Islamic Azad University, and Membership of young researchers club, IR
1 Lecture of Microbiology, Shahreza Branch, Islamic Azad University, and Membership of young researchers club, IR
Source
Indian Journal of Science and Technology, Vol 5, No S3 (2012), Pagination: 2425-2428Abstract
Para crystalline layer is a monomolecular outermost protein layer in bacteria and archiae, credit of protein or glycoprotein subunits and has crystalline biopolymer structure. Para crystalline -layer protects bacteria to phagocytosis and prohibit the entry of some biomolecule -for example antibiotics- and adhesion to matrix proteins, is one of virulence agents and layer producer. Para crystalline layer have application potential in biotechnology, molecular nanotechnology, and biomimetics. The research was performed with laboratory method in 2005-2007 years, in Azzahra hospital and Isfahan University. In this research, 26 Bacillus cereus strains were studied. Identification of bacteria, was performed with microbiological methods: staining, chemical test, using differential and selective media. Isolation of Bacillus cereus strains was performed on Selective Agar, and cultured in TSA, for 16 hours, under aerobic condition. Then subjected to the separation of surface proteins and electrophoresed along with molecular weight marker. Para crystalline layer in B. cereus has 97 KD MW. Out of 26 B. cereus strain, 14 strain produced Para crystalline layer and 12 strain don't have Para crystalline -layer. Out of 13 isolates from staff hand, 11 sample (84/6%) and from 13 isolates from hospital surfaces, 1 sample (7/7%) have produced Para crystalline layer.Keywords
Para Crystalline Layer, Bacillus cereus, Nosocomial InfectionsFull Text
References
- Hilliard NJ, Schelonka RL and Waites KB (2003) Bacillus cereus bacteremia in a preterm neonate. J. Clin. Microbiol. 41, 3441-3444.
- Jalalpoor S, Kasra Kermanshahi R, Noohi A and Zarkesh Esfahani H (2007) Study of ß-lactamase and S-layer layer production in some of isolated pathogen bacteria from clinical and environmental hospital samples. MSc thesis,Iran, Tehran, Islamic Azad University Science and Research Branch Tehran, Iran.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS, Zarkesh Esfahani H (2008) Study to Spreading Bacteria in How and Low Contact Surfaces in Hospital.9th Iranian Congress of Microbiology, Kerman, Iran. pp.208.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS, Zarkesh Esfahani H (2009) Survey and comparative bacterial spread pattern in staff hands and high and low contact hospital surfaces. Third Iranian Congress of Clinical Microbiology, Shiraz, Iran, pp.184.
- Jalalpoor S, Kasra Kermanshahi R, Nouhi AS, Zarkesh Esfahani H (2010) Survey Frequency of ß- lactamase Enzyme and Antibiotic Sensitivity Pattern in Isolated Pathogen Bacteria from Low and High Hospital Contact Surfaces. Pajuhandeh J. 15, 77-82.
- Kamp G and Kramer A (2004) Epidemiologic Background of Hand Hygiene and Evaluation of the Most Important Agents for Scrubs and Rubs. Clin. Microbiol. Rev. 17(4), 863-93.
- Kotiranta A, Haapasalo M and Kari K (1998) Surface Structure, Hydrophobicity, Phagocytosis ,and Adherence to Matrix Proteins of Bacillus cereus Cells with and without the Crystalline Surface Protein Layer. Infect. Immun. 66(10), 4895–4902.
- Kotiranta AK, Hitoshi I, Markus P, Haapasalo P and Kari L (1999) Radiation sensitivity of Bacillus cereus with and without a crystalline surface protein layer.FEMS Microbiol. Lett.179, 275-280.
- Mesnage S, Haustant M and Foue A (2001) A general strategy for identification of S-layer genes in the Bacillus cereus group :molecular characterization of such a gene in Bacillus thuringiensis subsp. galleriae NRRL 4045. J Microb.147,1343-51.
- Messner P, Steiner K, Zarschler K and Schaffer C (2008) S-layer nanoglycobiology of bacteria. Carbohydr. Res. 343(12),1934-51.
- Sambrook J and Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor,NY: Cold Spring Harbor Laboratory Press.
- Sara M (2001) Conserved anchoring mechanisms between crystalline cell surface S-layer proteins and secondary cell wall polymers in Gram-positive bacteria. Trends Microbiol. 9, 47–9.
- Sara M and Uwe B (2000) Sleytr.S-layer Proteins. J Bacteriol. 182(4), 859–68.
- Schaffer C and Messner P (2001) Glycobiology of surface layer proteins. Biochimie. 83, 591-99.
- Schaffer C and Paul M (2005) The structure of secondary cell wall polymers: how Gram-positive bacteria stick their cell walls together .Microbiol.15, 643–651.
- Sehulster L and Raymond YW (2003) Guidelines for Environmental Infection Control in Health-Care Facilities. U.S. Department of Health and Human Services Centers for Disease Control and Prevention (CDC). Atlanta GA 30333.
- Van der Zwet WC, Parlevliet GA, Savelkoul PH, Stoof J and Kaiser AM (2003) Outbreak of Bacillus cereus infection in a neonatal intensive care unit traced to balloons used in manual ventilation. J. Clin. Microbiol .38, 4131-6.
- Washington C, Stephen A, Janda W, Koneman E, Procop G, Schreckenberger P and Woods G (2006) Koneman's Color Atlas and Textbook of Diagnostic Microbiology, Sixth edition.USA: Lippincott wiiliams & wilkins, pp: 775-9.