Journal of Biological Control

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Biocontrol of Faba Bean Black Root Rot Caused by Fusarium solani Using Seed Dressing and Soil Application of Trichoderma harzianum


Affiliations
1 Ethiopian Institute of Agricultural Research, Plant Protection Research Center, P.O. Box 37, Ambo, Ethiopia
2 Ethiopian Institute of Agricultural Research, Holleta Agricultural Research Center, Holleta, Ethiopia
 

Black ischolar_main rot of faba bean is one of the most destructive diseases that causes up to 100% yield loss on farmers’ fields under severe conditions. As use of chemical fungicides for control of the disease is neither efficient nor economical, alternative options such as biological control need to be exploited. The antagonistic fungus Trichoderma harzianum was used as seed dressing or soil application treatment in combination with three varieties viz. Kasa (susceptible), Wolki (moderately resistant) and Wayu (resistant) in two consecutive cropping seasons in a sick plot. Each variety was sown with and without T. harzianum. There were a total of six treatments. The experimental design was randomized complete block design with three replications in a 2 x 3 factorial arrangement. Results showed that in the first year highly significant variations in the percentage of dead plants at harvesting stage were observed only due to varietal differences for soil treatment (P = 0.0001) and seed dressing (P< 0.0001) respectively. Percentage of dead plants of variety wayu was 26.1% and 30.8% in soil application and seed dressing treatments respectively. Similarly, 92.1% and 95.2% dead plants were observed for the susceptible variety Kassa in soil application and seed dressing treatments respectively. In the second year there was a significant interaction between varieties and Trichoderma treatments (P=0.0047, F=8.66, df= 2) with variety Wayu showing the least percentage of dead plants (56.58%) significantly differing from Kassa (93.86%) and Wolki (77.08%) in the soil treatments. It is concluded that use of T. harzianum in combination with a resistant variety such as Wayu is effective to reduce the incidence of faba bean ischolar_main rot caused by Fusarium solani. Future research should focus on studying the effects of combining different Trichoderma spp. on the disease as integrated with resistant varieties.

Keywords

Biocontrol, Root Rot, Faba Bean, Trichoderma harzianum, Fusarium solani.
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  • Biocontrol of Faba Bean Black Root Rot Caused by Fusarium solani Using Seed Dressing and Soil Application of Trichoderma harzianum

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Authors

Belay Habtegebriel
Ethiopian Institute of Agricultural Research, Plant Protection Research Center, P.O. Box 37, Ambo, Ethiopia
Anteneh Boydom
Ethiopian Institute of Agricultural Research, Holleta Agricultural Research Center, Holleta, Ethiopia

Abstract


Black ischolar_main rot of faba bean is one of the most destructive diseases that causes up to 100% yield loss on farmers’ fields under severe conditions. As use of chemical fungicides for control of the disease is neither efficient nor economical, alternative options such as biological control need to be exploited. The antagonistic fungus Trichoderma harzianum was used as seed dressing or soil application treatment in combination with three varieties viz. Kasa (susceptible), Wolki (moderately resistant) and Wayu (resistant) in two consecutive cropping seasons in a sick plot. Each variety was sown with and without T. harzianum. There were a total of six treatments. The experimental design was randomized complete block design with three replications in a 2 x 3 factorial arrangement. Results showed that in the first year highly significant variations in the percentage of dead plants at harvesting stage were observed only due to varietal differences for soil treatment (P = 0.0001) and seed dressing (P< 0.0001) respectively. Percentage of dead plants of variety wayu was 26.1% and 30.8% in soil application and seed dressing treatments respectively. Similarly, 92.1% and 95.2% dead plants were observed for the susceptible variety Kassa in soil application and seed dressing treatments respectively. In the second year there was a significant interaction between varieties and Trichoderma treatments (P=0.0047, F=8.66, df= 2) with variety Wayu showing the least percentage of dead plants (56.58%) significantly differing from Kassa (93.86%) and Wolki (77.08%) in the soil treatments. It is concluded that use of T. harzianum in combination with a resistant variety such as Wayu is effective to reduce the incidence of faba bean ischolar_main rot caused by Fusarium solani. Future research should focus on studying the effects of combining different Trichoderma spp. on the disease as integrated with resistant varieties.

Keywords


Biocontrol, Root Rot, Faba Bean, Trichoderma harzianum, Fusarium solani.

References





DOI: https://doi.org/10.18311/jbc%2F2016%2F15593