Journal of Biological Control

Open Access Open Access  Restricted Access Subscription Access

Analysis of Genetic Diversity among different Isolates of Beauveria bassiana by RAPD-PCR


Affiliations
1 Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
 

The genetic diversity of B. bassiana isolated from the different ecosystem of Tamil Nadu was analyzed. Totally fifteen isolates were used in this study. The genetic variability and relationship among 15 isolates were analyzed using 15 Random Amplified Polymorphic DNA (RAPD) markers. Among 15 primers used, six primers viz., OPA02, OPF01, OPX07, OPA03, OPZ19 and OPG19 showed 100 percent polymorphism. The relationships among the isolates were examined and represented as dendrogram by using UPGMA clusters. Results revealed that similarity coefficients of B. bassiana isolates based on RAPD markers ranged from 42.00 to 86.00 percent. The maximum of 85 percent similarity was observed between the isolates B23 and B24. All isolates have significantly different from one another. Overall we concluded that RAPD was found to be better in assessing genetic diversity among B. bassiana isolates.

Keywords

Beauveria Bassiana, Clustering, Genetic Diversity, RAPD, UPGMA.
User
Notifications

  • Bala Naik R, Ramakrishna Rao A, Murali Krishna T, Devaki K. 2015. Molecular characterization of Beauveria bassiana isolates through RAPD-PCR. Current Biotica. 9(2): 105–118.

  • Bidochka MJ, McDonald MA, St. Leger RJ, Roberts DW. 1994. Differentiation of species and strains of entomopathogenic fungi by random amplification of polymorphic DNA (RAPD). Curr Genet. 25: 107–113.

  • Lenka B, Landa Z, Lance S, Osborne, Curn V. 2002. Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD – PCR technique. Plant Prot Sci. 38: 11–12.

  • Carneiro AA, Gomes EA, Guimaraes CT, Fernandes FT, Carneiro NP, Cruz L. 2008. Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm. Pesqui Agropecu Bras. 43: 513–520.

  • Castrillo LA, Wiegmann BM, Brooks WM. 1999. Genetic variation in Beauveria bassiana populations associated with the darkling beetle, Alphitobius diaperinus. J Invertebr Pathol. 73: 269–275.

  • Cruz LP, Gaitan AL, Gongora CE. 2006. Exploiting the genetic diversity of Beauveria bassiana for improving the biological control of the coffee berry borer through the use of strain mixtures. Appl Microbio Biotechnol. 71: 918–926.

  • Gaitan A, Valderrama AM, Saldarriaga G, Velez P, Bustillo A. 2002. Genetic variability of Beauveria bassiana associated with the coffee berry borer Hypothenemus hampei and other insects. Mycol Res. 106: 1307–1314.

  • Garrido-Jurado I, Fernandez-Bravo M, Campos C, Quesada-Moraga E. 2015. Diversity of entomopathogenic Hypocreales in soil and phylloplanes of five Mediterranean cropping systems. J Invertebr Pathol. 130: 97–106.

  • Gomez KA, Gomez AA. 1984. Statistical Procedure for Agricultural Research. John Wiley and Sons., New York.

  • Imoulan A, Koraichi Ibnsouda S, El Meziane A. 2016. Molecular characterization and the effectiveness of native entomopathogenic Beauveria bassiana strains against adults of Mediterranean fruit fly (Ceratitis capitata). J Biosci Biotechnol Discov. 1: 6–16.

  • Jaccard P. 1912. The distribution of the flora in the alpine zone. New Phytol. 11: 37–50.

  • Jie J, Liangen S. 2010. RAPD analyse to detect genetic diversity of Beauveria bassiana from the silkworm , Bombyx mori in China. IVth International Conference on Bioinformatics and Biomedical Engineering, Chengdu, pp. 1–5.

  • Kaur G, Padmaja V. 2008. Evaluation of Beauveria bassiana isolates for virulence against Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) and their characterization by RAPD-PCR. Afr J Microbiol Res. 2: 299–307.

  • Kosir JM, Macpherson JM, Khachatourians GG. 1991. Genomic analysis of a virulent and a less virulent strain of the entomopathogenic fungus Beauveria bassiana using RFLP. Can J Microbiol. 37: 534–538.

  • Leal SC, Bertioli DJ, Butt TM, Peberdy JF. 1994. Characterization of isolates of the entomopathgenic fungus Metarhizium anisopliae by RAPD – PCR. Myco Res. 98(9): 1077–1081.

  • Meyling NV, Lubeck M, Buckley EP, Eilenberg J, Rehner SA. 2009. Community composition, host range and genetic structure of the fungal entomopathogen Beauveria in adjoining agricultural and seminatural habitats. Mol Ecol. 18: 1282–1293.

  • Ownley BH, Griffin MR, Klingeman WE, Gwinn KD, Moulton JK, Pereira RM. 2008. Beauveria bassiana: endophytic colonization and plant disease control. J Invertebr Pathol. 3: 267–270.

  • Prabhukarthikeyan SR, Karthikeyan G, Raguchander T. 2014. PCR based amplification of chitinase gene from native isolates of Beauveria bassiana. J Biological Control 28: 31–34.

  • Poeaim S, Hangtrakul S, Soytong K. 2014. Genetic diversity of Beauveria sp. isolated in Thailand. J Agric Technol. 10: 607–616.

  • Rohlf FJ. 1990. NTSYS-pc numerical taxanomy and multivariate analysis system, Version 1.8. Exeter Publ., Setauket, New York.

  • Shimazu M. 1993. Isolation, culturing and preservation of entomogenous fungi. pp. 192–222. In: Okada et al. (Eds.). Research Methods for Entomopathogens. Japan Plant Protection Association, Tokyo.

  • Tigano MS, Ana Cristina MM, Gomes Bruno, Sobral WS. 1995. Genetic variability among Brazilian isolates of the entomopathogenic fungus Metarhizium anisopliae. J Invert Pathol. 65: 206–210.

  • White TJ, Bruns T, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a guide to methods and applications In: Innis MA, Gelfand DH, Sninsky JJ, White TJ editors. Academic Press, San Diego. pp. 315–322.

  • Willaims JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18(22): 6531–6535.

  • Zolan ME, Pukkila PJ. 1986. Inheritance of DNA methyl¬ation in Coprinus cinereus. Molecular and Cell Biol. 6: 195–200.


Abstract Views: 310

PDF Views: 140




  • Analysis of Genetic Diversity among different Isolates of Beauveria bassiana by RAPD-PCR

Abstract Views: 310  |  PDF Views: 140

Authors

S. R. Prabhukarthikeyan
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
U. Keerthana
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
S. Archana
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
T. Raguchander
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

Abstract


The genetic diversity of B. bassiana isolated from the different ecosystem of Tamil Nadu was analyzed. Totally fifteen isolates were used in this study. The genetic variability and relationship among 15 isolates were analyzed using 15 Random Amplified Polymorphic DNA (RAPD) markers. Among 15 primers used, six primers viz., OPA02, OPF01, OPX07, OPA03, OPZ19 and OPG19 showed 100 percent polymorphism. The relationships among the isolates were examined and represented as dendrogram by using UPGMA clusters. Results revealed that similarity coefficients of B. bassiana isolates based on RAPD markers ranged from 42.00 to 86.00 percent. The maximum of 85 percent similarity was observed between the isolates B23 and B24. All isolates have significantly different from one another. Overall we concluded that RAPD was found to be better in assessing genetic diversity among B. bassiana isolates.

Keywords


Beauveria Bassiana, Clustering, Genetic Diversity, RAPD, UPGMA.

References





DOI: https://doi.org/10.18311/jbc%2F2017%2F15581