Journal of Biological Control

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Evaluation of Cry IIa Transgenic Chickpea Lines for Resistance to Helicoverpa armigera (Hubner) Using Detached Leaf Assay


Affiliations
1 Professor Jayashankar Telangana State Agricultural University (PJTSAU), Hyderabad - 500030, Telangana, India
2 Dr. Y S Parmar University of Horticulture and Forestry, Nauni, Solan - 173230, Himachal Pradesh, India
3 Acharya N.G Ranga Agricultural University (ANGRAU), Bapatla, Guntur - 522509, Andhra Pradesh, India
4 International Crop Research for Semiarid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana - 502324, India
 

Studies were conducted to evaluate transgenic chickpea lines encoding Cry IIa for resistance to Helicoverpa armigera. Significantly lower leaf damage was noticed in transgenic chickpea lines when compared to non-transgenic lines. Significant reduction in larval survival and weight gain were observed when H. armigera were fed on transgenic lines under laboratory conditions. Across the seasons (2011-12 and 2012-13), the transgenic chickpea lines BS5A.2(T2) 19-1P2 and BS5A.2(T2) 19-2P1 showed enhanced levels of resistance to H. armigera.

Keywords

Cry IIa, Detached Leaf Assay, Helicoverpa armigera, Transgenic Chickpea.
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Notifications

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  • Mehrotra M, Singh AK, Sanyal I, Altosaar I, Amla DV. 2011. Pyramiding of modified cry1Ab and cry1Ac genes of Bacillus thuringiensis in transgenic chickpea (Cicer arietinum L.) for improved resistance to pod borer insect Helicoverpa armigera. Euphytica 182: 87–102.

  • Sanyal I, Singh AK, Kaushik M, Amla DV. 2005. Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) with Bacillus thuringiensis cry1Ac gene for resistance against pod borer insect Helicoverpa armigera. Pl Sci. 168: 1135–1146.

  • Sharma HC. Pampapathy G, Dhillon MK, Smith R. 2005. Detached Leaf Assay to Screen for Host Plant Resistance to Helicoverpa armigera. J Econ Entomol. 98(2): 568–576.


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  • Evaluation of Cry IIa Transgenic Chickpea Lines for Resistance to Helicoverpa armigera (Hubner) Using Detached Leaf Assay

Abstract Views: 215  |  PDF Views: 124

Authors

O. Shaila
Professor Jayashankar Telangana State Agricultural University (PJTSAU), Hyderabad - 500030, Telangana, India
H. C. Sharma
Dr. Y S Parmar University of Horticulture and Forestry, Nauni, Solan - 173230, Himachal Pradesh, India
T. Ramesh Babu
Acharya N.G Ranga Agricultural University (ANGRAU), Bapatla, Guntur - 522509, Andhra Pradesh, India
S. P. Sharma
International Crop Research for Semiarid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana - 502324, India

Abstract


Studies were conducted to evaluate transgenic chickpea lines encoding Cry IIa for resistance to Helicoverpa armigera. Significantly lower leaf damage was noticed in transgenic chickpea lines when compared to non-transgenic lines. Significant reduction in larval survival and weight gain were observed when H. armigera were fed on transgenic lines under laboratory conditions. Across the seasons (2011-12 and 2012-13), the transgenic chickpea lines BS5A.2(T2) 19-1P2 and BS5A.2(T2) 19-2P1 showed enhanced levels of resistance to H. armigera.

Keywords


Cry IIa, Detached Leaf Assay, Helicoverpa armigera, Transgenic Chickpea.

References





DOI: https://doi.org/10.18311/jbc%2F2017%2F15551