Journal of Biological Control

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Detection of Insecticide Resistance and Mechanisms of Resistance in Field Populations of Chrysoperla zastrowi Sillemi (Neuroptera:Chrysopidae) Collected from Different Geographical Locations in India


Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, P.B. No. 2491, H.A. Farm Post, Hebbal, Banaglore - 560024, Karnataka, India
2 Regional Seri Cultural Research Station, Central Silk Board, Veeranam Road, Allikuttai Post, Vaikkalapattarai, Salem - 636003, Tamil Nadu, India
 

The toxic effect of commonly used insecticides in cotton fields was studied on 9 populations of Chrysoperla zastrowi sillemi (Esben-Petersen), an important predator of sucking pests collected in India. The dose mortality bioassay against 3-days old larvae was determined using three insecticides viz., endosulfan, fenvalerate and acephate by topical bioassay method. Mechanism of resistance to the above mentioned insecticides were determined without and with three metabolic inhibitors (synergists), viz., piperonyl butoxide (PBO), S,S,S-tributyl-phosphorotrithioate (DEF) and diethyl maleate (DEM). Among the populations, resistant ratios (RR) of CZS-8 was significantly higher i.e. 50.36., 66.11 and 277.51-fold for endosulfan, fenvalerate and acephate, respectively compared to susceptible population (CZS-10). The CZS-8 was selected for synergism study it showed higher LC50 values and resistance ratio for all three insecticides. It showed 8.97-fold, 18.49-fold and 6.38-fold increase in synergism ratio for endosulfan indicating the resistance was strongly synergised by PBO, DEF and DEM. Similarly for fenvalerate, CZS-8 showed 8.69-fold and 3.63-fold significant increase in synergism ratio by DEF and DEM, respectively and for acephate, CZS-8 showed 54.82-fold, 150.87-fold and 113.52-fold significant increase in synergism ratio indicating that the resistance could be due to cytochrome p-450, esterase and glutathione s- transferase activity. The study indicated that the field population of C. z. sillemi developed resistance to different groups of insecticides. Among different geographical populations, CZS-8 collected from Sriganganagar, was recorded as most resistant.

Keywords

Chrysoperla zastrowi Sillemi, Cytochrome P450, Esterase, Glutathione–S-Transferase Insecticide Resistance.
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  • Detection of Insecticide Resistance and Mechanisms of Resistance in Field Populations of Chrysoperla zastrowi Sillemi (Neuroptera:Chrysopidae) Collected from Different Geographical Locations in India

Abstract Views: 260  |  PDF Views: 119

Authors

T. Venkatesan
ICAR-National Bureau of Agricultural Insect Resources, P.B. No. 2491, H.A. Farm Post, Hebbal, Banaglore - 560024, Karnataka, India
S. Helen Mahiba
Regional Seri Cultural Research Station, Central Silk Board, Veeranam Road, Allikuttai Post, Vaikkalapattarai, Salem - 636003, Tamil Nadu, India
S. K. Jalali
ICAR-National Bureau of Agricultural Insect Resources, P.B. No. 2491, H.A. Farm Post, Hebbal, Banaglore - 560024, Karnataka, India
S. L. Ramya
ICAR-National Bureau of Agricultural Insect Resources, P.B. No. 2491, H.A. Farm Post, Hebbal, Banaglore - 560024, Karnataka, India
M. Prathibha
ICAR-National Bureau of Agricultural Insect Resources, P.B. No. 2491, H.A. Farm Post, Hebbal, Banaglore - 560024, Karnataka, India

Abstract


The toxic effect of commonly used insecticides in cotton fields was studied on 9 populations of Chrysoperla zastrowi sillemi (Esben-Petersen), an important predator of sucking pests collected in India. The dose mortality bioassay against 3-days old larvae was determined using three insecticides viz., endosulfan, fenvalerate and acephate by topical bioassay method. Mechanism of resistance to the above mentioned insecticides were determined without and with three metabolic inhibitors (synergists), viz., piperonyl butoxide (PBO), S,S,S-tributyl-phosphorotrithioate (DEF) and diethyl maleate (DEM). Among the populations, resistant ratios (RR) of CZS-8 was significantly higher i.e. 50.36., 66.11 and 277.51-fold for endosulfan, fenvalerate and acephate, respectively compared to susceptible population (CZS-10). The CZS-8 was selected for synergism study it showed higher LC50 values and resistance ratio for all three insecticides. It showed 8.97-fold, 18.49-fold and 6.38-fold increase in synergism ratio for endosulfan indicating the resistance was strongly synergised by PBO, DEF and DEM. Similarly for fenvalerate, CZS-8 showed 8.69-fold and 3.63-fold significant increase in synergism ratio by DEF and DEM, respectively and for acephate, CZS-8 showed 54.82-fold, 150.87-fold and 113.52-fold significant increase in synergism ratio indicating that the resistance could be due to cytochrome p-450, esterase and glutathione s- transferase activity. The study indicated that the field population of C. z. sillemi developed resistance to different groups of insecticides. Among different geographical populations, CZS-8 collected from Sriganganagar, was recorded as most resistant.

Keywords


Chrysoperla zastrowi Sillemi, Cytochrome P450, Esterase, Glutathione–S-Transferase Insecticide Resistance.

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DOI: https://doi.org/10.18311/jbc%2F2017%2F16333