Journal of Biological Control

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Chitinase Activity and Virulence of Different Isolates of Beauveria bassiana, Metarhizium anisopliae and Lecanicillium Spp


Affiliations
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, India
 

In order to identify promising isolates with higher chitinase activity, 122 entomofungal isolates comprising of Beauveria bassiana (58), Metarhizium anisopliae (33), Lecanicillium lecanii (15), Lecanicillium attenuatum (11), L. longisporum (3) and L. muscarium (2) isolated from different insect hosts/soils of India were studied. The partially purified proteins from the isolates were subjected to chitinase activity and were estimated by measuring the release of reducing saccharides (one NAGA Unit) from colloidal chitin spectrophotometrically at 582 nm (A582). Forty nine isolates of B. bassiana showed chitinase activity ranging from 21 to 182 μg/ml, with the highest enzyme activity by the isolate PDBC-Bb-5a. Thirty three isolates of M. anisopliae exhibited chitinase activity ranging from 23 to 144 μg/ml and the highest (144 μg/ml) was by the isolate Ma-4. Among the 15 isolates of L. lecanii tested, three isolates viz., Vl-7, Vl-24a, Vl-25a had high chitinase activities ranging between 100 and 126μg/ml. Vl-22 isolate of L. attenuatum, Vl-24 of L. longisporum and Vl-8 of L. muscarium showed higher activities (90, 110 and 117μg/ml respectively). Bioassay studies with these isolates on cowpea aphid, Aphis craccivora in glass house indicated higher nymphal mycosis ranging from 72.3-83.0%.

Keywords

Entomopathogenic Fungi, Chitinase Activity, NAGA Unit, Cowpea Aphid, Aphis craccivora, Virulence.
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  • Chitinase Activity and Virulence of Different Isolates of Beauveria bassiana, Metarhizium anisopliae and Lecanicillium Spp

Abstract Views: 285  |  PDF Views: 128

Authors

B. Ramanujam
National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, India
M. Balachander
National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, India
G. Roopa
National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, India
R. Rangeshwaran
National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, India
Karmakar Pritam
National Bureau of Agriculturally Important Insects, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, India

Abstract


In order to identify promising isolates with higher chitinase activity, 122 entomofungal isolates comprising of Beauveria bassiana (58), Metarhizium anisopliae (33), Lecanicillium lecanii (15), Lecanicillium attenuatum (11), L. longisporum (3) and L. muscarium (2) isolated from different insect hosts/soils of India were studied. The partially purified proteins from the isolates were subjected to chitinase activity and were estimated by measuring the release of reducing saccharides (one NAGA Unit) from colloidal chitin spectrophotometrically at 582 nm (A582). Forty nine isolates of B. bassiana showed chitinase activity ranging from 21 to 182 μg/ml, with the highest enzyme activity by the isolate PDBC-Bb-5a. Thirty three isolates of M. anisopliae exhibited chitinase activity ranging from 23 to 144 μg/ml and the highest (144 μg/ml) was by the isolate Ma-4. Among the 15 isolates of L. lecanii tested, three isolates viz., Vl-7, Vl-24a, Vl-25a had high chitinase activities ranging between 100 and 126μg/ml. Vl-22 isolate of L. attenuatum, Vl-24 of L. longisporum and Vl-8 of L. muscarium showed higher activities (90, 110 and 117μg/ml respectively). Bioassay studies with these isolates on cowpea aphid, Aphis craccivora in glass house indicated higher nymphal mycosis ranging from 72.3-83.0%.

Keywords


Entomopathogenic Fungi, Chitinase Activity, NAGA Unit, Cowpea Aphid, Aphis craccivora, Virulence.

References