Journal of Biological Control

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Evaluation of Biocontrol Potential of Metarhizium anisopliae Strains Against Larvae and Adults of Aedes eegypti (l.)


Affiliations
1 Division of Biochemical Sciences, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 400 008, MH, India
2 Laboratory of Entomology, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 400 008, MH, India
 

In the present study, we exposed larvae and adult females of Aedes aegypti (L.) to fungal suspensions of three virulent strains of Metarhizium anisopliae (Metsch.), viz., M34412, M34311 and M81123. The strains resulted in 7 to 37% survival as a result of fungal infection over the 8 d test period in case of adults and 2 to 90% survival for larvae. Mean survival period varied between 3 and 4 d for treated adults, whilst control survival exceeded 28 d. Values of LC50 varied from 5.92×103 conidia/ml for M34412, 3.49×104 conidia/ml for M34311 and 5.12×105 conidia/ml for M81123. Based on virulence and stability, the most promising strain, M. anisopliae M34412 was used for lethal exposure time determination. An exposure time of only 4 h was necessary to cause 50% mortality. Supplementation with M. verrucaria chitinase enzyme increased the larval mortality at lower conidial concentrations.

Keywords

Entomopathogen, Metarhizium anisopliae, Aedes aegypti, Biocontrol, Diptera, Culicidae.
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  • Evaluation of Biocontrol Potential of Metarhizium anisopliae Strains Against Larvae and Adults of Aedes eegypti (l.)

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Authors

Manisha Kapoor
Division of Biochemical Sciences, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 400 008, MH, India
Pushpa Vijay Pawar
Laboratory of Entomology, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 400 008, MH, India
Mary Joseph
Laboratory of Entomology, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 400 008, MH, India
Avalokiteswar Sen
Laboratory of Entomology, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 400 008, MH, India
Mukund Vinayak Deshpande
Division of Biochemical Sciences, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 400 008, MH, India

Abstract


In the present study, we exposed larvae and adult females of Aedes aegypti (L.) to fungal suspensions of three virulent strains of Metarhizium anisopliae (Metsch.), viz., M34412, M34311 and M81123. The strains resulted in 7 to 37% survival as a result of fungal infection over the 8 d test period in case of adults and 2 to 90% survival for larvae. Mean survival period varied between 3 and 4 d for treated adults, whilst control survival exceeded 28 d. Values of LC50 varied from 5.92×103 conidia/ml for M34412, 3.49×104 conidia/ml for M34311 and 5.12×105 conidia/ml for M81123. Based on virulence and stability, the most promising strain, M. anisopliae M34412 was used for lethal exposure time determination. An exposure time of only 4 h was necessary to cause 50% mortality. Supplementation with M. verrucaria chitinase enzyme increased the larval mortality at lower conidial concentrations.

Keywords


Entomopathogen, Metarhizium anisopliae, Aedes aegypti, Biocontrol, Diptera, Culicidae.

References