Journal of Biological Control

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Effect of Temperature on the Life Cycle of Entomopathogenic Nematodes, Steinernema abbasi and Heterorhabditis indica


Affiliations
1 Department of Nematology, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, Haryana, India
 

In vitro studies on the effect of temperature on the life cycle of Steinernema abbasi and Heterorhabditis indica revealed that IJs were able to penetrate Galleria mellonella at temperature range between 20° and 30°C for both the species. The maximum number of IJs of S. abbasi and H. indica emerge from G. mellonella larvae at 30°C and 25°C, respectively, and time (days) taken for emergence of first and second generation individuals of S. abbsi and H. indica on G. mellonella were observed to be 3-4 and 7-8 days and 3-4 and 6-10 days, respectively. The number of male and female amphimictic generation was 25IJs for both EPNs. However the sex ratio was 1: 14.9 at 25°C, 1: 11.6 at 20°C and 1: 11.3 was minimum at 25°C (12.5: 32.5) followed by 20°C (1: 14.7) and 30°C (1: 16.6) S. abbasi and H. indica, respectively. As the temperature decreased, the emergence of IJs also decreased significantly.

Keywords

Entomopathogenic Nematodes(EPNs), Heteorhabditis indica, Life Cycle, Steinernema abbasi and Temperature.
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  • Effect of Temperature on the Life Cycle of Entomopathogenic Nematodes, Steinernema abbasi and Heterorhabditis indica

Abstract Views: 286  |  PDF Views: 132

Authors

B. S. Sunanda
Department of Nematology, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, Haryana, India

Abstract


In vitro studies on the effect of temperature on the life cycle of Steinernema abbasi and Heterorhabditis indica revealed that IJs were able to penetrate Galleria mellonella at temperature range between 20° and 30°C for both the species. The maximum number of IJs of S. abbasi and H. indica emerge from G. mellonella larvae at 30°C and 25°C, respectively, and time (days) taken for emergence of first and second generation individuals of S. abbsi and H. indica on G. mellonella were observed to be 3-4 and 7-8 days and 3-4 and 6-10 days, respectively. The number of male and female amphimictic generation was 25IJs for both EPNs. However the sex ratio was 1: 14.9 at 25°C, 1: 11.6 at 20°C and 1: 11.3 was minimum at 25°C (12.5: 32.5) followed by 20°C (1: 14.7) and 30°C (1: 16.6) S. abbasi and H. indica, respectively. As the temperature decreased, the emergence of IJs also decreased significantly.

Keywords


Entomopathogenic Nematodes(EPNs), Heteorhabditis indica, Life Cycle, Steinernema abbasi and Temperature.

References