Journal of Biological Control

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Evaluation of Epiphytic Microflora as Antagonists of Red Rot Pathogen, Colletotrichum falcatum in Sugarcane under Subtropical Conditions


Affiliations
1 Division of Microbiology, Indian Institute of Agricultural Research, New Delhi, 110 012, India
2 Organic Chemistry Laboratory, Division of Plant Physiology and Biochemistry, Indian Institute of Sugarcane Research, Lucknow, Uttar Pradesh, India
 

Epiphytic microflora isolated from different plant parts of healthy sugarcane plants at different growth stages were assessed for their ability to control fungal red rot pathogen of sugarcane both in vitro and in vivo. Microbial population was higher in the month of August with highest on cane stalk followed by buds and dry leaves. A total of 112 bacterial isolates representing 16 morphotypes, 63 fungal and 35 actinomycete isolates representing 7 morphotypes each and 21 mould isolates of 3 morphotypes were purified from the collection of isolates obtained from different plant parts at different periods of plant growth stage and environment condition. These 231 microbial isolates were screened in vitro for their antifungal activity against Colletotrichum falcatum using dual culture technique. None of the mould isolates could inhibit C. falcatum growth, while 12 bacterial isolates, 4 fungal isolates and 5 actinomycete isolates were found to be antagonistic to C. falcatum. Biochemical and 16SrRNA partial sequence characterization of 12 bacterial isolates led to identification of Pseudomonas, Serratia, Bacillus, Paenibacillus, Gluconacetobacter, Serratia marscens (DQ144501) and Paenibacillus macerans (DQ144502). Based on colony morphology, spore arrangement and structure characteristics the fungal and actinomycete isolates were identified to of Aspergillus, Trichoderma and Penicillium and of Streptomyces and Saccharopolyspora sp., respectively. The strongest inhibition of C. falcatum was obtained with actinomycete isolates and Serratia marscens both under in vitro and in vivo conditions, that can serve to be part of integrated disease management (IDM) of red rot in sugarcane in subtropics.

Keywords

Epiphytes, Biocontrol, Colletotrichum falcatum, Serratia marscens, Aspergillus sp., Trichoderma sp., Penicillium sp., Streptomyces sp., Saccharopolyspora sp., Sugarcane.
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  • Alexander KC, Viswanathan R. 1996. Major diseases affecting sugarcane production in India and recent experiences in quarantine pp. 46–48. In: Croft, B.J., Piggin, C.M., Wallis, E.S., Hogarth, D.M. (Eds.), Sugarcane Germplasm Conservation and exchange, Proceedings 67. Australian Centre for International Agricultural Research, Canberra.

  • Campant S, Reiter B, Sessitsch A, Novak J, Clement C, Barka EA. 2005. Endophytic colonisation by Vitis vinifera L. by plant growth promoting bacterium Burkholderia sp. starin P5 JN, Appl Env Microbiol, 71: 1685–1693.

  • Chalutz E, Droby S, Wilson CL. 1988. Microbial protection against post harvest disease of citrus fruit. Phytoparasitica 16: 195–196.

  • Chand-Goyal T, Spotts RA. 1996. Control of post harvest pear diseases using natural saprophytic yeast colonists and their combination with a low dosage of thiabendazole. Postharvest Biol Technol 7: 51–64.

  • Chand-Goyal T, Spotts RA. 1997. Biological control of post harvest diseases of apple and pear under semicommercial and commercial conditions using three saprophytic yeasts. Biol Control 10: 199–06.

  • Droby S, Cohen L, Daus A, Weiss B, Horev B, Chalutz E, Katz H, Keren-Tzur M, Shachnai A. 1998. Commercial testing of Aspire: a yeast preparation for the biological control of postharvest decay of citrus. Biol Control. 12: 97–101.

  • Glick BR, Bhashan Y. 1997. Genetic manipulation of plant growth promoting bacteria to enhance biocontrol of pathogens, Biotechnol Adv 15: 353–378.

  • Huang CJ, Chen CY. 2008. Synergistic interaction between chitinase Chic Wand fungicides against plant fungal pathogens, J Microbiol Biotechnol. 18(4): 784–787.

  • Janisiewicz WJ. 1987. Postharvest biological control of blue mold on apples. Phytopathol. 77: 481–485.

  • Janisiewicz WJ, Usall J, Bors B. 1992. Nutritional enhancement of biocontrol of blue mold on apples. Phytopathol. 82: 1364–1370.

  • John GH. Bergey’s Manual of Determinative Bacteriology 1984. David Hendricks Berey, Wlliams & Wilkins, 1989 pp. 2648.

  • Mercier J, Lindow SE. 2000. Role of leaf surface sugars in colonization of plants by bacterial epiphytes. Appl Env Microbiol. 60: 927–931.

  • Nautiyal CS, Mehta S, Singh SB. 2006. Biological control and plant growth promoting bacillus strains from milk. J Microbiol Biotech. 16: 184–192.

  • Padmanaban P, Mohanraj D, Viswanathan R, Rao MM, Prakasam N, Jothi R Alexander, KC. 1996. Differential interaction of sugarcane clones to pathotypes of Colletotrichum falcatum Went. Sugarcane 4: 16–20.

  • Raajmakers JM, Vlami M, Desouza JT. 2002. Antibiotic production by bacterial bioagents, Antonie Leewenhoek, 81(28): 528–548.

  • Sehgal JL, Mandal DR, Mandal C, Vadivelu S. 1990. Agro-Ecological Regions of India. NBSS & LUP Nagpur, India.

  • Sholberg PL, Gaunce AP. 1995. Fumigation of fruits with acetic acid to prevent post harvest decay. Hortscience 30: 1271–1275.

  • Singh K, Singh RP. 1989. Red rot. pp. 169–188. In: Ricaud C, Egan BT, Gillaspie Jr, AG, Hughes CG. (Eds.), Major Diseases of Sugarcane. Elsevier, Amsterdam.

  • Sinigaglia M, Corbo MR, Altiri C, Massa S. 2002. Response surface model for effects of temperature, water activity and pH on germination of Bacillus cereus spores. J Food Safety, 22: 121–133.

  • Snedecor GW, Cochran WG. 1967. Statistical methods. Oxford/IBH Publ, India.

  • Viswanathan R, Samiyappan R. 1999a. Induction of systemic resistance by plant growth promoting rhizobacteria in sugarcane against red rot disease. Sugar Tech. 1: 67–76.

  • Viswanathan R, Mohanraj D, Padmanaban P. 1998. Comparison of three testing methods for evaluation of resistance to red rot caused by Colletotrichum falcatum in sugarcane (Saccharum officinarum). Indian J Agri Sci 68: 226–230.

  • Viswanathan R, Samiyappan R. 1999b. Identification of antifungal chitinases from sugarcane. ICAR News 5(4): 1–2.


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  • Evaluation of Epiphytic Microflora as Antagonists of Red Rot Pathogen, Colletotrichum falcatum in Sugarcane under Subtropical Conditions

Abstract Views: 330  |  PDF Views: 134

Authors

Archna Sumana
Division of Microbiology, Indian Institute of Agricultural Research, New Delhi, 110 012, India
B. Pushpa Singh
Organic Chemistry Laboratory, Division of Plant Physiology and Biochemistry, Indian Institute of Sugarcane Research, Lucknow, Uttar Pradesh, India

Abstract


Epiphytic microflora isolated from different plant parts of healthy sugarcane plants at different growth stages were assessed for their ability to control fungal red rot pathogen of sugarcane both in vitro and in vivo. Microbial population was higher in the month of August with highest on cane stalk followed by buds and dry leaves. A total of 112 bacterial isolates representing 16 morphotypes, 63 fungal and 35 actinomycete isolates representing 7 morphotypes each and 21 mould isolates of 3 morphotypes were purified from the collection of isolates obtained from different plant parts at different periods of plant growth stage and environment condition. These 231 microbial isolates were screened in vitro for their antifungal activity against Colletotrichum falcatum using dual culture technique. None of the mould isolates could inhibit C. falcatum growth, while 12 bacterial isolates, 4 fungal isolates and 5 actinomycete isolates were found to be antagonistic to C. falcatum. Biochemical and 16SrRNA partial sequence characterization of 12 bacterial isolates led to identification of Pseudomonas, Serratia, Bacillus, Paenibacillus, Gluconacetobacter, Serratia marscens (DQ144501) and Paenibacillus macerans (DQ144502). Based on colony morphology, spore arrangement and structure characteristics the fungal and actinomycete isolates were identified to of Aspergillus, Trichoderma and Penicillium and of Streptomyces and Saccharopolyspora sp., respectively. The strongest inhibition of C. falcatum was obtained with actinomycete isolates and Serratia marscens both under in vitro and in vivo conditions, that can serve to be part of integrated disease management (IDM) of red rot in sugarcane in subtropics.

Keywords


Epiphytes, Biocontrol, Colletotrichum falcatum, Serratia marscens, Aspergillus sp., Trichoderma sp., Penicillium sp., Streptomyces sp., Saccharopolyspora sp., Sugarcane.

References