A Modified Method for Mass Multiplication of Pasteuria penetrans (thorne) Sayre & starr

A simple method to mass multiply Pasteuria penetrans (Thorne) Sayre&Starr an obligate parasite of plant parasitic nematodes, was worked out by modifying the method defined by Stirling and Wachtel (1980). With the increase in seed rate/pot, there was an increase in ischolar_main content/pot and number of Meloidogyne incognita (Kofoide&White) Chitwood females which in turn contributed to an increase in number of infected females and spore number/g ischolar_main. This method not only enhanced spore production efficiency/pot but also reduced the inherent disadvantage of maintaining and handling a large number of plants under individual pot (in vivo) conditions.