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UNDIRWADE, D. B.
- Influence of Varying Temperatures on Toxicity of Biorationals Against Diamondback Moth,Plutella Xylostella L.
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PDF Views:85
Authors
Affiliations
1 Department of Agricultural Entomology, Dr. Panjabrao Deshmukh Krushi Vidyapeeth, Akola - 444104, Maharashtra, IN
1 Department of Agricultural Entomology, Dr. Panjabrao Deshmukh Krushi Vidyapeeth, Akola - 444104, Maharashtra, IN
Source
Journal of Biological Control, Vol 35, No 2 (2021), Pagination: 82-87Abstract
Effect of various temperatures on the efficacy of five biorationals, viz. Beauveria bassiana (1.15% WP), Metarhizium anisopliae (1.15% WP), Bacillus thuringiensis (0.5% WP), Azadirachtin (300 ppm) and Spinosad (45 SC) in terms of median lethal concentration (LC<sub>50</sub>) value was evaluated against diamondback moth, Plutella xylostella larvae. The toxicity data for biorationals against P. xylostella on the basis of larval mortality revealed that M. anisopliae (1.15% WP), B. thuringiensis (0.5% WP), Azadirachtin (300 ppm) and Spinosad (45 SC) registered higher effectiveness (lower LC<sub>50</sub> value) at 25(sup>o</sup> C whereas, increase in temperature led to declined efficacy of biorationals; whereas, use of entomopathogenic fungi, B. bassiana (1.15% WP) was most effective at 30<sup>o</sup> C and revealed lower effectiveness (higher LC<sub>50</sub> value) at 25<sup>o</sup> and 35<sup>o</sup> C temperatures. Data on the influence of temperature on toxicity of biorationals to P. xylostella on the basis of adult emergence revealed lowest LC<sub>50</sub> value for B. thuringiensis (0.5% WP) at 25<sup>o</sup> C and the efficacy decreased with increase in the temperature, whereas, M. anisopliae (1.15% WP), B. bassiana (1.15% WP), Azadirachtin (300 ppm) and Spinosad (45 SC) were most effective at 30<sup>o</sup> C and the higher LC<sub>50</sub> value were evident at temperature above 30<sup>o</sup> C, indicating the reduced efficacy of biorationals with increase in temperature beyond 30<sup>o</sup> C or preference for lower temperature regimes under laboratory conditions.Keywords
Biorationals, Diamondback moth, LC50, temperature regimes.Full Text
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- Efficacy of HearNPV formulations against Helicoverpa armigera at different sunlight exposure period
Abstract Views :148 |
PDF Views:97
Authors
Affiliations
1 Oilseeds Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola – 444 104, Maharashtra, IN
2 Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola – 444 104, Maharashtra, IN
3 Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola – 444 104, Maharashtra, IN
1 Oilseeds Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola – 444 104, Maharashtra, IN
2 Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola – 444 104, Maharashtra, IN
3 Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola – 444 104, Maharashtra, IN
Source
Journal of Biological Control, Vol 35, No 2 (2021), Pagination: 107-113Abstract
Experiment was conducted at Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during 2011- 2013 to see the effect of natural sunlight (UV) on HearNPV formulation. Experiment was laid out in Completely Randomized Block Design replicated thrice. The aqueous and dry form of HearNPV formulations were prepared by using additives viz. Tinopal an optical brightener and silver nano particle and evaluated their capability to protect NPVs from Ultra Violet rays. HearNPV formulation were sprayed uniformly on the potted chickpea plants during noon hours. Twigs were collected at thirty minutes, One hour, One and half hours and two hours after spraying and kept in petriplates. The laboratory reared second instar larvae of H. armigera were released on each treated twig. Larval mortality was recorded at 4, 7 and 10 days after treatment. An aqueous form of HearNPV formulation, HearNPV + Silver Nano Particles @ 8 µl /ml of HearNPV + Tinopal 1% + Sucrose 1% (T1) recorded 83.04% larval mortality at ten days after spraying which was at par with HearNPV alone unirradiated (84.21 % larval mortality) when exposed to sunlight up to one hour. Among the lyophilized form of formulations, HearNPV + Silver Nano Particles @ 8µl/ml of HearNPV + Tinopal 1% + Sucrose1% (T6) when exposed to sunlight up to one hour recorded 84.80% larval mortality at ten days after spraying which was at par with which was at par with HearNPV alone unirradiated (T12) (85.38% larval mortality) and higher than HearNPV alone irradiated. Both aqueous and lyophilized form of HearNPV showed decreasing larval mortality as compared to HearNPV alone unirradiated when exposed to sunlight up to one and half and two hours. Lyophilized HearNPV formulations recorded higher larval mortality as compared to aqueous form of HearNPV formulations at all sunlight exposure period.Keywords
HearNPV formulations, Helicoverpa armigera, UV Rays.Full Text
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