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Rajashekhar, M.
- In vitro Studies on Bactericidal Activity and Sensitivity Pattern of Isolated Marine Microalgae against Selective Human Bacterial Pathogens
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Authors
Affiliations
1 Department of Biosciences, Mangalore University, Mangala Gangothri, Karnataka– 574199, IN
1 Department of Biosciences, Mangalore University, Mangala Gangothri, Karnataka– 574199, IN
Source
Indian Journal of Science and Technology, Vol 2, No 8 (2009), Pagination: 16-23Abstract
Ten microalgae cultured under controlled condition were tested for their antimicrobial activity against the selective bacterial pathogens in compliance with paper disk method. Five different solvents were used for the extraction. Isochrysis galbana showed overall inhibition of [16.22%] followed by Chlorella marina [14.43%], Nannochloropsis oculata [14.07%], Dunaliella salina [13.91%] and Pavlova lutheri [13.17%]. These five microalgal strains were further investigated to examine concentration dependent microbicidal activity using tube dilution method. Microalgal strains were also investigated with agar-well diffusion method to understand the efficacy of antimicrobial principles against various bacterial pathogens. The findings in this study reveal that optimal activity is maintained by butanol extracts on Gram-positive bacteria; ethanol and petroleum ether extracts on both Gram-positive and Gram-negative bacteria; methanol extracts on Gram-negative organisms. Chloroform extracts, on the other hand did not show any significant antimicrobial activity.Keywords
Bactericidal, Disk Diffusion, Microalgae, Antibiotics, PathogensFull Text
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- Interaction of Carbon Nanotubes Reinforced Hydroxyapatite Composite with Bacillus subtilis, P. aeruginosa and C. albicans
Abstract Views :212 |
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Authors
P. Khalid
1,
M. A. Hussain
2,
P. D. Rekha
1,
C. Sanal
3,
S. Suraj
3,
M. Rajashekhar
3,
V. B. Suman
4,
Sangappa
5,
A. B. Arun
1
Affiliations
1 Yenepoya Research Centre, Yenepoya University, Mangalore, 575018, IN
2 Department of Electrical and Computer Engineering, King Abdulaziz University, Jeddah, 215895, SA
3 Department of Bioscience, Mangalore University, Mangalore 574199, IN
4 Kasturba Medical College, Manipal University, Mangalore 575004, IN
5 Department of Studies in Physics, Mangalore University, Mangalore 574199, IN
1 Yenepoya Research Centre, Yenepoya University, Mangalore, 575018, IN
2 Department of Electrical and Computer Engineering, King Abdulaziz University, Jeddah, 215895, SA
3 Department of Bioscience, Mangalore University, Mangalore 574199, IN
4 Kasturba Medical College, Manipal University, Mangalore 575004, IN
5 Department of Studies in Physics, Mangalore University, Mangalore 574199, IN
Source
Indian Journal of Science and Technology, Vol 7, No 5 (2014), Pagination: 678–684Abstract
Hydroxyapatite (HA), as a bone mineral component, has been an attractive bioceramic for the reconstruction of hard tissues. However, its poor mechanical properties, including low fracture toughness and tensile strength, have been a significant challenge to the application of HA for the replacement of load-bearing and/or large bone defects.Hydroxyapatite (HA) composite is reinforced with high purity and well-functionalized Multiwalled Carbon Naotubes (MWCNT>98 wt%) having an average diameter of 15 nm.The cellular response of f-MWCNT, MWCNT-HA composites were examined to model gram positive and gram negative Bacteria B. subtilis, P. aeruginosa and yeast C. albicans.
Ca(NO3)2.4H2O and (NH4)2HPO4 were used to synthesize HA in situ. MWCNT were functionalized by heating at 1000C in 3:1 ratio of H2SO4 and HNO3 for 60 m with stirring and dispersed in Sodium Dodecyl Benzene Sulphonate (SDBS) by sonication. Hydroxy Apatite (HA) particles were produced in MWCNTs solution by adding Ca(NO3)2.4H2O and (NH4)2HPO4 under vigorously stirring conditions. The composite were dried and washed in distilled water followed by heat treatment at 2500C to obtain CNT-HA powder. Using FTIR, FESEM and EDS does physicochemical characterization of the composite material.
The interaction of f-MWCNT and MWCNT-HA were tested on Bacillus subtilis, P. aeruginosa and C. albicans. The zone of inhibition and MIC studies were carried out with a concentration range from 62.5 – 1000 μg/ml. The test result shows no zone of inhibition and MIC > 1000 μg/ml on bacteria and yeast. This result provides further evidence that the bio-nano interface can be developed for Carbon Nanotubes reinforced Hydroxyapatite composites for load-bearing bone implants, drug delivery and diagnostic applications.
Keywords
Hydroxyapatite, MIC, MWCNTs, NanocompositesFull Text