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Padmanaban, P.
- Identification of Antifungal Proteins from Fungal and Bacterial Antagonists against Colletotrichum falcatum Causing Sugarcane Red Rot
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PDF Views:117
Authors
Affiliations
1 Crop Protection Division, Sugarcane Breeding Institute, Coimbatore 641 007, Tamil Nadu, IN
1 Crop Protection Division, Sugarcane Breeding Institute, Coimbatore 641 007, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 26, No 1 (2012), Pagination: 49-54Abstract
Effective fungal and bacterial antagonists having hyperparasitism and antibiosis mode of antagonistic activity against Colletotrichum falcatum were selected based on the suppression of pathogen growth and production of cell wall lytic enzyme chitinase. The effective strains were utilized to identify the sources of antifungal proteins suppressing enzymes and toxin, the pathogenicity determinants of Colletotrichum falcatum during their interection The effective biocontrol agents identified against C.falcatum inhibited the production of extracellular enzymes/proteins of the pathogen and also detoxified/inactivated the toxin/enzymes, when the antagonists were grown along with pathogen or its metabolites in liquid cultures as well as at the point of entry, where it failed to elicit characteristic symptoms on the host. Final outcome of the study resulted in identification of high molecular proteins specifically produced by the antagonists to inactivate the pathogenic metabolites in SDS-PAGE.Keywords
Sugarcane, Colletotrichum falcatum, Secondary Metabolites, Antifungal Proteins.Full Text
References
- Cabib E. 1987. The synthesis and degradation of chitin. In: Advances in enzymology and related areas in molecular biology 3: 59–101.
- Cruz J, Gallego, J M., Lora I A, Benetiz A, Ihobell A. 1992. Isolation and characterization of the three chitinases from T. harzianum, Eur J Biochem. 206: 859–867.
- Hsu S C, Lockwood J L. 1975. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol. 29: 422–426.
- Kapat A, Zimand Y, Elad Y. 1998. Effect of two isolates of Trichoderma harzianum on the activity of hydrolytic enzymes produced by Botrytis cinerea. Physiol Mol Plant Pathol. 52.
- Malathi P, Viswanathan R, Padmanaban P, Mohanraj D, Ramesh S.A. 2000a. Microbial detoxification of Colletotrichum falcatum toxin. Curr Sci. 83 (6): 745-749.
- Malathi P, Viswanathan R, Padmanaban P, Mohanraj D., Ramesh S A. 2002b. Compatibility of biocontrol agents with fungicides against red rot disease of sugarcane. Sugar Technol. 4 (3&4): 131–136.
- Malathi P, Viswanathan R, Ramesh, S A, Padmanaban, P, Mohanraj D. 2006. Leaf bioassay – A simple technique for studying sugarcane red rot pathogen interaction. SBI Newslet. 25 (1): 2–3.
- Malathi P, Padmanaban P, Viswanathan R, Mohanraj D. 2008. Interaction between Colletotrichum falcatum pathotypes and biocontrol agents. Arch Phytopathol Pl Prot. 41(5): 311–317.
- Malathi P, Padmanaban P, Viswanathan R, Mohanraj D. 2010. Identification of pathogenicity determinants in Colletotrichum falcatum causing sugarcane red rot and its suppression by antifungal microbes. Proceedings of 9th Joint Convention of STAI and SISSTA held from 19th to 21st August’ 2010 at Chennai.
- Marra P, Ambrosino P, Vinale F, Woo S L, Ciliento R, Lanzuise S, Ferraioli S, Soriente I, Gigante S, Turra D, Scala F, Lorito M. 2006. Study of the three–way interaction between Trichoderma atroviride, plant and fungal pathogens by using a proteomic approach. Curr Genet. 50: 307–321.
- Mohanraj D, Padmanaban P, Karunakaran M. 2002. Association of phytotoxin produced by Colletotrichum falcatum Went in the red rot disease of sugarcane. Sugarcane May/June, 21–23 and 30.
- Nallathambi P, Mohanraj D, Padmanaban P. 2000. Biocontrol of red rot caused by Colletotrichum falcatum in sugarcane. Proc Indian Phytopathol Soc Golden Jubilee., Vol. I, 315–316.
- Okamoto S, Masaru S, Yasuvuki K, Gento T, Isao F, Yutaka E, Makato O, Hiromichi N, Shohei S. 2001. Inhibitory effect of aflastatin A on melanin of biosynthesis by Colletotrichum lagenarium. Microbiol. 147: 2623–2628.
- Panopolous MT, Peet R C. 1985. The molecular genetics of plant pathogenic bacteria and their plasmids. Ann Rev Phytopathol. 23: 381–419.
- Sriram S, Raguchander S, Babu S, Nandakumar R, Shanmugham V, Vidyasekaran P, Balasubramanian P, Samiyappan R. 2000. Inactivation of phytotoxin produced by the rice sheath blight pathogen Rhizoctonia solani. Canadian J Microbiol. 46: 520–524.
- Sivan A, Chet J. 1989. Degradation of fungal cell walls by lytic enzymes of T. harzianum. J Gen Microbiol. 115: 675–682.
- Viswanathan R. 2010. Plant Disease: Red rot of sugarcane, New Delhi, Anmol Publishers. P.301.
- Biological Control of Sugarcane Root Rot Disease
Abstract Views :200 |
PDF Views:117
Authors
Affiliations
1 Division of Crop Protection, Sugarcane Breeding Institute, Coimbatore-641 007, IN
1 Division of Crop Protection, Sugarcane Breeding Institute, Coimbatore-641 007, IN
Source
Journal of Biological Control, Vol 4, No 2 (1990), Pagination: 135-136Abstract
Every year at Sugarcane Breeding Institute, 5 to 6 lakhs sugarcane seedlings are raised from true seed (fluff) which is an essential step in the development of new commercial clones. At the initial stages of seedling growth, ischolar_main rot disease caused by Pythium graminicolum Sub. results in loss of large number of valuable seedlings. Frequent drenching of mercurial fungicide Agallol, has not effectively controlled the ischolar_main rot disease but it suppressed beneficial microflora in the soil. Many soil borne pathogens have been reported to be controlled by the use of antagonistic organism Trichoderma spp. (Sivan et al., 1984; Papavizas, 1985; Mukhopadhyay, 1987). Attempts have been made to control ischolar_main rot disease in nursery seedbeds by employing antagonistic organism Trichoderma viride on a large scale (Padmanaban and Alexander, 1987; 1990). The possible mechanism of control have been studied and the results are presented in this paper.Keywords
Pythium graminicolum, Biological Control, Mechanism of Biological Control.Full Text