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Evaluation of Antioxidant and Antimicrobial Potential of Green Synthesized Ag Nanoparticles from Ethanolic Leaf Extract of Thespesia populnea


Affiliations
1 Department of Biotechnology, Bannari Amman Institute of Technology, Sathymangalam, Erode District, Tamil Nadu, India
2 Department of Biotechnology, Sri Shakthi Institute of Engineering & Technology, Coimbatore, Tamil Nadu, India
 

Thespesia populnea is a variety of small tree found mostly in the tropical regions of the world belonging to the family of Malvaceae. According to ancient and traditional medicine such as Ayurveda this plant is used to treat various diseases such as bloody diarrhea, fracture healing, urinary tract problem, abdomen swelling, pleurisy, skin disorders, high fever and menstrual issues. This study focuses on the evaluation of antioxidant and antimicrobial activities of synthesized silver nanoparticles from aqueous leaf extract of Thespesia populnea plant. Reducing capability of synthesized nanoparticles was examined by carrying out various antioxidant assays such as reduction of DPPH substrate through radical scavenging. It showed the maximum reduction of DPPH as 92.77% at 120 &#956g/mL concentration. At 120 &#956g/mL concentration, phosphomolybdenum showed highest contraction percentage ranging 51.47 % and followed by Fe3+ reduction at 28.78 %. Studies on antimicrobial activity has revealed that the maximum zone of inhibition was exhibited by Proteus vulgaris with distinct zone formation up to 19 mm diameter.

Keywords

Antioxidant, Antimicrobial, DPPH· Radical, Phosphomolybdenum
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  • Fosberg FR, Sachet M-H. Thespesia populnea (L.) Solander ex
  • Correa and Thespesia populneoides (Roxburgh) Kosteletsky (Malvaceae). Smithsonian Contributions to Botany. 1972;
  • :13.
  • Girach RD, Aminuddin AM, Brahmam M, Misra MK. Native phytotherapy among rural population of district Bhadrak Orissa. In: Ethnobiology in human welfare: Abstracts of the fourth international congress of ethnobiology, Lucknow, Uttar Pradesh, India; 1994. p. 85.
  • Ilavarasan R, Vasudevan M, Anbazhagan S, Venkataraman S, Sridhar SK. Hepatoprotective activity of Thespesia populnea bark extracts against carbon tetrachloride – induced liver toxicity in rats. Nat Prod Sci. 2003; 9(2):83–6.
  • Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011; 48(4):412–22. https://doi.org/10.1007/s13197-011-0251-1. PMid: 23572765. PMCid: PMC3551182.
  • Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337–41. https://doi.org/10.1006/abio.1999.4019.
  • PMid: 10222007.
  • Sivaraj C, Aashinya Y, Sripriya R, Arumugam P. Antioxidant activities and thin layer chromatography analysis of aqueous extract of tubers of Drynaria quercifolia (L). J. Sm. Free Radic Antioxid. 2018; 8(1):26–31. https://doi.org/10.5530/fra.2018.1.5
  • Obeidat M, Shatnawi M, Al-alawi M, Al-Zu’bi E, Al-Dmoor
  • H, Al-Qudah M, et al. Antimicrobial activity of crude extracts of some plant leaves. Res. J. Microbiol. 2012;7(1):59–67.
  • https://doi.org/10.3923/jm.2012.59.67 .
  • Jayasri A, Prasad PE, Padmaja K, Kumar BDPK, Gnanaprakash M, Kavitha K. Antibacterial activity of Thespesia populnea mediated nanoparticles. Int J Curr Microbiol App Sci. 2021; 10(02):913–18. https://doi.org/10.20546/ijcmas.2021.1002.107 .
  • Elshawy OE, Helmy EA, Rashed LA. Preparation, characterization, and in vitro evaluation of the antitumor activity of the biologically synthesized silver nanoparticles. Advances in Nanoparticles. 2016; 5:149–66. https://dx.doi.org/10.4236/anp.2016.52017 .
  • Chandran RP, Manju S, Vysakhi MV, Shaji PK, Nair GA. Antibacterial and antifungal activities of Thespesia populnea leaf extracts against human pathogens. Int J Pharma Tech Res. 2014; 6(1):290–7.
  • Parthasarathy R, Singh A, Bhowmik D. Pharmacognostical studies on Thespesia populnea leaf. Res J Pharmacognosy and Phytochem. 2016; 8(1):16–20. https://doi.org/10.5958/0975-4385.2016.00004.2
  • Shivakumar H, Prakash T, Rao RN, Swamy BHMJ, Nagappa AN. Wound healing activity of the leaves of Thespesia populnea. J Nat Remedies. 2007; 7(1):120–4.
  • Jayasri A, Prasad PE, Padmaja K, Kumar BDPK, Gnanaprakash M, Sonali M. In vitro and in vivo safety evaluation of Thespesia populnea mediated nanoparticles. Pharma Innovation. 2021; 10(1):09–11.

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  • Evaluation of Antioxidant and Antimicrobial Potential of Green Synthesized Ag Nanoparticles from Ethanolic Leaf Extract of Thespesia populnea

Abstract Views: 164  |  PDF Views: 81

Authors

S. Charulatha
Department of Biotechnology, Bannari Amman Institute of Technology, Sathymangalam, Erode District, Tamil Nadu, India
H. V. Dharrunya
Department of Biotechnology, Bannari Amman Institute of Technology, Sathymangalam, Erode District, Tamil Nadu, India
R. Dhanamurugan
Department of Biotechnology, Bannari Amman Institute of Technology, Sathymangalam, Erode District, Tamil Nadu, India
R. Devadharshini
Department of Biotechnology, Bannari Amman Institute of Technology, Sathymangalam, Erode District, Tamil Nadu, India
Debanjoy Paul
Department of Biotechnology, Bannari Amman Institute of Technology, Sathymangalam, Erode District, Tamil Nadu, India
M. E. Sinduja
Department of Biotechnology, Bannari Amman Institute of Technology, Sathymangalam, Erode District, Tamil Nadu, India
J. Bindhu
Department of Biotechnology, Sri Shakthi Institute of Engineering & Technology, Coimbatore, Tamil Nadu, India

Abstract


Thespesia populnea is a variety of small tree found mostly in the tropical regions of the world belonging to the family of Malvaceae. According to ancient and traditional medicine such as Ayurveda this plant is used to treat various diseases such as bloody diarrhea, fracture healing, urinary tract problem, abdomen swelling, pleurisy, skin disorders, high fever and menstrual issues. This study focuses on the evaluation of antioxidant and antimicrobial activities of synthesized silver nanoparticles from aqueous leaf extract of Thespesia populnea plant. Reducing capability of synthesized nanoparticles was examined by carrying out various antioxidant assays such as reduction of DPPH substrate through radical scavenging. It showed the maximum reduction of DPPH as 92.77% at 120 &#956g/mL concentration. At 120 &#956g/mL concentration, phosphomolybdenum showed highest contraction percentage ranging 51.47 % and followed by Fe3+ reduction at 28.78 %. Studies on antimicrobial activity has revealed that the maximum zone of inhibition was exhibited by Proteus vulgaris with distinct zone formation up to 19 mm diameter.

Keywords


Antioxidant, Antimicrobial, DPPH· Radical, Phosphomolybdenum

References





DOI: https://doi.org/10.18311/jnr%2F2022%2F26226