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Vyas, Niraj
- Assessment of Testosterone Biogenesis Enhancement by a Lupeol Derivative Separated from Seeds of Hygrophila spinosa T. Ander in vitro
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PDF Views:249
Authors
Affiliations
1 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
2 Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
1 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
2 Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
Source
Journal of Natural Remedies, Vol 21, No 4 (2021), Pagination: 317-322Abstract
The present study aimed towards assessing the isolated lupeol derivative in vitro for testosterone biogenesis enhancement by the action on isolated rat leydig cells. Studies were carried out to separate a novel bioactive molecule from an Indian medicinal plant, Hygrophila spinosa, used as an aphrodisiac and spermatogenic for years. Unsaponifiable bioactive fraction, prepared from seeds of the plant, was subjected to isolation of phytoconstituents by column and flash chromatography. The isolated compound was characterized by spectral studies as lupeol methyl ether. Later, it was subjected to in vitro studies using isolated leydig cells from rat for its action. Different amounts of isolated compound were incubated with leydig cells for 3 hours and concentration of testosterone after incubation was considered as a parameter. Dehydroepiandrosterone was used as positive control in the studies. The results indicated that the isolated compound was active and enhanced the testosterone biogenesis, as evident from increased amount of testosterone found in a medium treated with compound. This was the first report included isolation, chemical characterization and biological activity of Lupeol methyl ether from Hygrophila spinosa T. Ander.Keywords
Hygrophila spinosa, Lupeol, Leydig Cells, Steroids, Tri-terpenoids, Testosterone.Full Text
References
- Nadkarni KM. Indian Materia Medica, Vol. 713, Popular Prakashan, Bombay; 1976
- Bhavmishra BS, Nighantu B. Pandit Shri Brahma Sankar Mishra with the Vidyotani Hindi Commentatry. Chaukhambha Sanskrit Sansthan, Varanasi; 2005.
- Kirtikar KR, Basu BD. Indian Medicinal Plants. Vol. 2, International book distributors, Dehradun. 1991. 1182 p.
- Basu NK, Rakhit S. Investigation of the seeds of Asteracantha longifolia Nees. Indian J Pharm. 1957; 19:282–4.
- Chauhan NS, Sharma V, Dixit VK. Effect of Asteracanthalongifolia seeds on the sexual behaviour of male rats. Nat Prod Res. 2011; 25(15):1423–31. https://doi.org/10.1080/14786410802588493
- Vyas NY, Raval MA. Aphrodisiac and spermatogenic potential of alkaloidal fraction of Hygrophila spinosa T. Ander in rats. J Ethnopharmacol. 2016; 194:947– 53. https://doi.org/10.1016/j.jep.2016.10.080
- Vyas NY, Raval M. Effect of unsaponifiable fraction of seeds of Hygrophila spinosa T. Ander on testosterone production of rat leydig cells in vitro. Asian J Pharm Clin Res. 2016; 9(6):184–6. https://doi.org/10.22159/ajpcr.2016.v9i6.14049
- MdYaseen K, Vimal K, Anuradha G, Niraj V, Siddharth P, Amee B. Reverse pharmacognosy in new drug discovery. Curr Pharma Res J. 2007; 1(5):31–6.
- Vyas N, Khan M, Panchal S, Butani A, Kumar V. Supercritical fluid technology: An unlimited frontier in herbal research. Int J Pharm Sci Nanotechnol. 2009; 1(4):303–8. https://doi.org/10.37285/ijpsn.2008.1.4.10
- Vyas N, Gamit K, Raval M, Patel S. Isolation and chemical characterization of bioactive alkaloid from Argyreia speciosa Linn. having action on isolated rat leydig cells. Asian J Pharm Clin Res. 2019; 12(10):276–80. https://doi.org/10.22159/ajpcr.2019. v12i10.34789
- Muhtadi FJ. Analytical profiles of drug substances and excipients. Vol. 23, Academic Press. Elsevier; 1994. p. 153–228. https://doi.org/10.1016/S0099-5428(08)60603-6
- Silverstein RM, Bassler GC, Morrill TC. Spectroscopy of organic compounds, New York, Chichester, Brisbane. Toronto, Singapore: John Wiley & Sons Inc; 2009.
- Urolithiasis: HPTLC Method for Quantitative Detection of Rutin and Quercetin in an Herbal Plant
Abstract Views :155 |
PDF Views:94
Authors
Affiliations
1 Department of Pharmacology, Indukaka Ipcowala College of Pharmacy, New Vallabh Vidyanagar, Anand - 388121, Gujarat, IN
2 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Changa, Anand - 388421, Gujarat, IN
3 Department of Pharmacology, L.M. College of Pharmacy, Ahmedabad - 380058, Gujarat, IN
1 Department of Pharmacology, Indukaka Ipcowala College of Pharmacy, New Vallabh Vidyanagar, Anand - 388121, Gujarat, IN
2 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Changa, Anand - 388421, Gujarat, IN
3 Department of Pharmacology, L.M. College of Pharmacy, Ahmedabad - 380058, Gujarat, IN
Source
Journal of Natural Remedies, Vol 22, No 3 (2022), Pagination: 371 - 379Abstract
Anti-inflammatory, Hypoglycemic, Hepatoprotective, Antihyperlipidemic, Anti-Ulcerative, Cardioprotective Stimulant, Sedative, Hypnotic, Anticonvulsant Activity, Memory Retention, Stroke Preventive Activity, Antimicrobial Activity, Antimycobacterial, Antiviral, Larvicidal, Antiparasitic, Chemopreventative, Chemo-modulatory, Anticancer, Cytoprotective activities were found in a variety of herbal plants, including Ocimum basilicum. The purpose of this study was to design and develop a new HPTLC method that was accurate, precise and cost-effective for simultaneous measurement of rutin and quercetin in a hydroalcoholic extract of Ocimum basilicum seeds. The mobile phase was Toluene: Ethyl Acetate: Methanol: formic acid (6:4:3:1, v/v/v/v/v) and densitometric scanning was performed at 254 nm. Merck TLC aluminium sheets of silica gel 60 F254, (10 x 10 cm) with a thickness of 250 mm was used as stationary phase. At a wavelength of 254 nm, rutin and quercetin were detected. The constituents were resolved satisfactorily, with Rf values of 0.25 ± 2.01 for rutin and 0.80 ± 0.64 for quercetin, respectively. Linearity (300-1300 ng/spot for rutin and quercetin) was used to test the method’s accuracy and reproducibility. For both analytes, intra- and inter-day precision, as evaluated by coefficient of variation, was less than 3%. For rutin, the detection and quantification limits were 46.52 and 140.96 ng/spot, respectively, and for quercetin, they were 81.79 and 247.84 ng/spot. The proposed approach was found to be precise, accurate, repeatable, and specific, and it could be used to measure quercetin and rutin in samples at the same time.
Keywords
Densitometry, Ocimum basilicum, Quercetin, High-performance Thin-layer Chromatography (HPTLC), Rutin, Validation.Full Text
References
- Schreiner M, Huyskens-Keil S. Phytochemicals in fruit and vegetables: Health promotion and postharvest elici- tors. Cri Rev Plant Sci. 2006; 25:267-78. https://doi. org/10.1080/07352680600671661
- Soleas GJ, Grass L, Josephy PD, Goldberg DM, Diamandis EP. A comparison of the anticarcinogenic properties of four red wine polyphenols. Clin Biochem. 2002; 35:119-24. https:// doi.org/10.1016/S0009-9120(02)00275-8
- Middleton EM, Teramura AH. The role of flavonol gly- cosides and carotenoids in protecting soybean from ultraviolet-B damage. Plant Physiol. 1993; 103:741-52. https://doi.org/10.1104/pp.103.3.741. PMid:12231976. PMCid:PMC159044
- Hein KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants:
- Chemistry, metabolism and structure-activity relation- ships. J Nutr Biochem. 2002; 13(10):572-84. https://doi. org/10.1016/S0955-2863(02)00208-5
- Bors W, Heller W, Michel C, Saran M. Flavonoids as antioxidants: determination of radical-scavenging effi- ciencies. Method Enzymol. 1990; 186:343-55. https://doi. org/10.1016/0076-6879(90)86128-I
- Dixon RA, Dey PM, Lamb CJ. Phytoalexins: enzymology and molecular biology. Adv Enzymol RAMB.1983; 55:1-136.
- Rice-Evans CA, Miller NJ, Bolwell PG, Broamley PM, Pridham JB. The relative antioxidant activities of plant- derived polyphenolic flavonoids. Free Radical Res. 1995; 22(4):375-83. https://doi.org/10.3109/10715769509145649. PMid:7633567
- Agati G, Azzarello E, Pollastri S, Tattini M. Flavonoids as antioxidants in plants: location and functional signifi- cance. Plant Sci. 2012; 196:67-76. https://doi.org/10.1016/j. plantsci.2012.07.014. PMid:23017900
- Du F, Zhang F, Chen F, Wang A. Advances in microbial heter- ologous production of flavonoids. Afr J Microbiology Res. 2011; 5(18):2566-74. https://doi.org/10.5897/AJMR11.394
- Middleton EJ. Effect of plant flavonoids on immune and inflammatory cell function. Adv Exp Med Biol. 1998; 439:175-82. https://doi.org/10.1007/978-1-4615-5335- 9_13. PMid:9781303
- Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. Scientific World J. 2013; 2013:1- 16. https://doi.org/10.1155/2013/162750. PMid:24327805. PMCid:PMC3845396
- Nijveldt RJ, Nood EL, Van Horn DEC, Boelens PG, Van Norren K, van Leeuwen PAM. Flavonoids: A review of prob- able mechanisms of action and potential applications. Am J Clin Nutr. 2001; 74(4):418-25. https://doi.org/10.1093/ ajcn/74.4.418. PMid:11566638
- Bilal A, Jahan N, Ahmed A, Bilal SN, Habib S, Hajra S. Phytochemical and pharmacological studies on Ocimum basilicum linn — A review. Int J Curr Res. 2012; 4(23).
- International Conference on Harmonization. Validation of Analytical Procedures Methodology Q2B; 1996.
- Method for determination of forskolin in crude drug and pharmaceutical dosage form. Chromatographia. 2008; 67:441-47. https://doi.org/10.1365/s10337-008-0521-x
- Alam P, Ali M, Ahmad SA. A validated HPLC method for estimation of cordifolioside A in Tinospora cardifolia, Miers and marketed formulations. J Chromatogr Sci. 2009; 47: 910-13. https://doi.org/10.1093/chromsci/47.10.910. PMid:19930804
- Parveen R, Baboota S, Ahmad S, Ali J, Ahuja A. Stability- indicating HPTLC method for quantitative estimation of silybin in bulk drug and pharmaceutical dosage form. Biomed Chromatogr. 2010; 24:639-47. https://doi. org/10.1002/bmc.1340. PMid:19816854
- Rozylo JK, Janicka M. Different planar techniques for predic- tion of solute retention in column liquid chromatography. J Planar Chromatogr. 1996; 9:418-24.
- Weins C, Hauck HE. Advances and developments in thin layer chromatography. LC-GC Int. 1996; 4:455-71.
- Poole CF, Poole SK. Instrumental thin layer chromatography. Anal Chem. 1994; 66:27A-37A. https://doi.org/10.1021/ ac00073a001
- Sejal P, Niraj V. Validated spectrofluorimetric method for estimation of piperine in an ayurvedic formulation. Asian J Pharm Clin Res. 2012; 5(4):231-233.
- Niraj V, Sangita P. Development and Validation of RP-HPLC Method for Simultaneous estimation of Nebivolol and Indapamide in Pharmaceutical Dosage Form. Asian J Pharm Anal. 2014; 4(3):98-102.