Journal of Natural Remedies

R. G. Mali , S. G. Mahajan , A. A. Mehta

Abstract

This research work was taken up to investigate the anthelmintic and antimicrobial potential of the stem bark of Bauhinia variegata Linn. The crude ethanolic extract of the plant was evaluated for anthelmintic activity using Indian earthworm Pheretima posthuma and Ascardia galli as test worms. Various concentrations (10-100 mg/ml) of ethanolic extract were tested in the bioassay, which involved determination of time of paralysis (P) and time of death (D) of the worms. Piperazine citrate (10mg/ml) included as reference standard. Antimicrobial activity of the crude ethanolic extract was evaluated by agar disc diffusion method and Minimum Inhibitory Concentration (MIC) was determined by broth dilution method by using some bacterial and fungal strains of microorganism. Ciprofloxacin (5μg/ml) and Cotrimazole (25μg/ml) were used as reference standard for the antibacterial and antifungal studies respectively. The ethanolic extract showed promising anthelmintic and antimicrobial activities and results were comparable with the standards included in the study.

Keywords

Bauhinia variegata, Anthelmintic Activity, Antimicrobial Activity, Piperazine Citrate, Ciprofloxacin, Cotrimazole

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R. G. Mali , J. C. Hundiwale , R. S. Gavit , K. S. Patil , M. V. Kulkarni

Abstract

Obejective: To study the effect of hydroalcoholic extract of aerial parts of Achyranthes aspera on neutrophil phagocytic function . Methods: The different concentrations (25,50 and 100 mg / ml ) of extract of aerial parts of Achyranthes aspera was subjected to study its effect on different in vitro methods of phagocytosis such as neutrophil locomotion and chemotaxis, in vitro immunostumulant activity by phyagocytosis of killed Candida albicans and qualitative nitro blue tetrazolium test using human neutrophils. Results: This preliminary study revealed that Achyranthes aspera extract has stimulated chemotactic, phagocytic and intracellular killing potency of human neutrophils at the concentration range of 25 - 100 mg / ml. Conclusion: From the results obtained it can be observed that the hydroalcoholic extract of Achyranthes aspera stimulates cell-mediated immune system by increasing neutrophil phagocytic function.

Keywords

Achyranthes Aspera, Neutrophils, Phagocytosis, Chemotaxis

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