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Journal of Natural Remedies, Vol 5, No 2 (2005), Pagination: 96-101
Abstract
Objective: Develop a method to quantify a mixture of isoshinanolone and epi- isoshinanolone (ISMIX) as a specific marker for Plumbago indica L. in a commercial herbal drug, "Dasamoolarishtaya" (DMA). Materials and Methods: The ISMIX content in different DMA samples were analyzed by TLC fluorodensitometry. Isoshinanolone isolated from the ischolar_main of P.indica was used as the standard. The precision and accuracy of the method were determined. Results: The concentrations of ISMIX in different samples of DMA varied widely from < 0.0012 mg/ml (detection limit of method) to 0.045 mg/ml. The precision was 1.52 % CV (n = 4) and recoveries of 99.2 - 100.8 % were obtained in standard addition recovery experiments. Conclusions: The method described is inexpensive, rapid and is of acceptable precision and accuracy for routine quality control work.
Keywords
Fluorodensitometry, Isoshinanolone, epi- Isoshinanolone, Quality Control, Standardization, Herbal Drugs
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