Medico-Legal Update

Jassim Mohamed Suleiman ¹, Omar-Althani Shareef Saed ¹, SMA AL-Kubaisi ², Mustafa Salah Hasan ², Maher Saber Owain ¹, Mohammed Ali Hussein ², Ahmed Sami Jarad ²

Affiliations
1 Department of Internal and Preventive Medicine, College of Vet. Med., University of Tikrit, IQ
2 Department of Internal and Preventive Medicine, College of Vet. Med., University of Fallujah, IQ

Abstract

This study aimed to detect eae and Stx genes of thirty two E. coli O157:H7 isolates recovered from calves. These isolates were isolated and identify by traditional methods of culturing and latex agglutination test from fecal swabs of four diarrheic calves and twenty eight apparently healthy calves. The eae and Stx genes were detected by a realtime PCR applied on the extracted bacterial DNA. The results showed that 19 isolates had stx1 gene, 10 isolates had stx2 gene and 17 isolates had eae gene. It is concluded that molecular characterization by using real time PCR is a good test for confirming infection with E. coli O157:H7 and detection of their eae and Stx genes

Keywords

Real time, PCR, stx, eae, Calves, E. coli O157:H7

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