In Vitro Anti-Oxidant Study of Herbal Extract Mixture by Nitric oxide and DPPH Method

Herbalism is a traditional medicine or folk medicine practice based on the use of plants or it's extracts. Herbal extract mixture produce synergetic activity compared to individuals, it was confirmed by its in- vitro antioxidant activity. Herb-herb combinations have been used in practice for thousands of years, yet scientific evidence of their therapeutic benefits is lacking.An increase demand for this , the present work examine thepotential of Herbal extract mixture. Mixtures were prepared in different combination (aqueous extract of Terminalia belerica : aqueous extract of Solanum xanthocarpum) ratio of 10:90, 20:80, 30 :70,40:60,50:50,60:40,70:30,80:20,90:10. All ratios were subjected to nitric oxide scavenging assay to find out the effective combinational mixture. The ratio 70:30 (Terminalia belerica : Solanum xanthocarpum ) showed maximum free radical scavenging property. The present results suggest that traditional crude drugs might be potent and novel therapeutic agents for scavenging of NO and the regulation of pathological conditions caused by excessive NO and its oxidation product. These findings may also help to explain, at least in part, certain pharmacological activities of crude drugs, especially anti-infection and anti-inflammatory activities.