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In-Silico and in-Vitro Evaluation of Xanthine Oxidase Inhibition of Zingiber officinalae for Hypouricemic Activity


Affiliations
1 Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
2 Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University) Chennai, India
     

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Zingiber officinale (ZO), traditionally known for its remedial potential, was examined on phytoconstituents analysis, antioxidant and xanthine oxidase inhibition by in-vitro analysis, followed by an in-silico study to identify the biological rationale. Results show that ethanolic extract of Zingiber officinale illustrated potential antioxidant to that of standard. DPPH ZO (IC50=42.09ug/mL), DPPH butylhydroxyltoluene (IC50=66.67ug/mL), nitric oxide ZO (IC50=28.36ug/mL), nitric oxide ascorbic acid (IC50=42.87𝜇g/mL). Zingiber officinale extract inhibited the xanthine oxidase activity (IC50 = 188.5ug/mL) compared to allopurinol (IC50=499.2ug/mL), which showed competitive inhibition. The in-silico docking studies of the major phytoconstituents revealed by GC-MS analysis from this extract suggest that the inhibitory activity may be due to the combined effect of these compounds. These results suggest substantial reason for the evaluation of active compounds in in-vivo system for the management and treatment of gout as well as in the oxidative stress conditions.

Keywords

Zingiber officinale, Anti-Oxidant assays, Molecular Docking, Hypouricemic.
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  • Ravindran PN, Nirmal BK. Ginger: the genus Zingiber. Medicinal and Aromatic Plant–industrial profile. CRC Press, Boca Raton, Florida. 2005.

  • Wallace KL, Riedel AA, Joseph-Ridge N, Wortmann R. Increasing prevalence of gout and hyperuricemia over 10 years among older adults in a managed care population. The Journal of rheumatology. 31(8); 2004:1582-7.

  • Triplitt CL, Reasner CA, Isley WL, Di Pirio R, Talbert L, Yee GC. Pharmacotherapy: A Pathophysiologic Approach.(2008): 1285-320

  • Shafer SL, Fiset P. Clinical Pharmacy and therapeutics.5th ed. Maryland: William and Wilkins. 1992.

  • Collin A, Pennington S, Lammon CB. Clinical Drug Therapy: Rationales for Nursing Practice (Field Guide).8th edition. USA:Lippincott Williams and Wilkins. 2006.

  • Zhao X, Zhu JX, Mo SF, Pan Y, Kong LD. Effects of cassia oil on serum and hepatic uric acid levels in oxonate-induced mice and xanthine dehydrogenase and xanthine oxidase activities in mouse liver. JEthnopharmacol. 103(3);2006: 357-65

  • Bustanji Y, Hudaib M, Tawaha K, Mohammad M, Almasri I, Hamed S, Oran S. In vitro xanthine oxidase inhibition by selected Jordanian medicinal plants. J. Pharm. Sci.1; 2011:1-8.

  • Harborne JB Modern methods of Phytochemical analysis, (1998). Chapman and Hall, Madras, 227-230

  • Hwang, B. Y., Kim, H. S., Lee, J. H., Hong, Y. S., Ro, J. S., Lee, K. S., and Lee, J. J. . Antioxidant Benzoylated Flavan-3-ol Glycoside from Celastrusorbiculatus. Journal of natural products, 64(1); 2001: 82-84.

  • Mensor, L.I., Menezes, F.S., Leitao, G.G., Reis, A.S., dos santos, T., Coube, C.S.,Leitao, S.G., Screening of Brazilian plants extracts for antioxidants activity by the use of DPPH freeradical method. Phytother. Res., 15; 2001:127-130.

  • Sumanont, Y.; Murakami, Y.; Tohda, M.; Vajragupta, O.; Matsumoto, K.; Watanabe, H. Evaluation of the nitric oxide radical scavenging activity of manganese complexes of curcumin and its derivative. Biol. Pharm. Bull., v.27,n.2; 2004: p.170-173.

  • Garrat, D.C. (1964) The quantitative analysis of drugs, Third Edition, Chapman and Hall Limited, Japan 456.

  • Bustanji Y, Hudaib M, Tawaha K, Mohammad KM, Almasri I, Hamed S, Oran S. In vitro xanthine oxidase inhibition by selected Jordanian medicinal plants. Jordan Journal of Pharmaceutical Sciences. 4(1);2011:

  • Nguyen M, Awale S, Tezuka, Tran Q, Watanabe H, Kadota S. Xanthine oxidase inhibitory activity of Vietnamese medicinal plants. Biol Pharm Bull. 27(9);2004: 1414-1421.

  • Murugaiyah V, Chan KL. Mecanisms of antihyperuricemia effect of Phillanthusniruri and its lignan constituents. Ethnopharm. 124; 2009: 233-239.

  • Roohbakhsh A, Shamsara J, Khayyat MH, Karimi G. Inhibition of xanthine oxidase by some Iranian plant remedies used for gout. Pharmacologyonline. 3;2009: 1031-1036z.

  • Apaya K, Hernandez C. Xathine oxidase inhibition of selected Philippine medical plants. Journal of Medicine Plants Research. 5(2);2011:289-292.

  • Azmi SMN, Jamal P, Amid A.Xanthine oxidase inhibitory activity from potential Malaysian medicinal plant as remedies for gout.International Food Research Journal. 19(1); 2012: 159-165.

  • Toyama JI. A simple method for the isolation of xanthine oxidase from milk. The Journal of Biochemistry. 17(1); 1933:131-3.

  • Podlogar JA1, Verspohl EJ.-Phytother Res. 2012 Mar;26(3):3336. doi: 10.1002/ptr.3558. Epub 2011 Jun 23.

  • "Diogo V. FonsĂŞca, Paula R. R. Salgado, FabĂ­ola L. de Carvalho, Mirian Graciela S. S. Salvadori, AntĂ´niaRosângela S. Penha, Fagner C. Leite, ClĂłvis JosĂ© S. Borges, Marcia R. Piuvezam, Liana ClĂ©bia de MoraisPordeus, DamiĂŁo P. Sousa, Reinaldo N. Almeida. Nerolidol exhibits antinociceptive and antiinflammatory activity: involvement of the GABAergic system and proinflammatory cytokines. Fundamental and clinical pharmacology. 30(1); 2016: Pages 14–22."

  • Kim CS1, Kawada T, Kim BS, Han IS, Choe SY, Kurata T, Yu R.- Cell Signal,15(3); 2003:299-306.

  • Tjendraputra E, Tran VH, Liu-Brennan D, Roufogalis BD, Duke CC, Bioorg Chem. 29(3); 2001:156-63.

  • Reiter E1, Jiang Q, Christen S.-Mol Aspects Med. 28(5-6): 2007:668-91. Epub 2007 Jan 11.

  • Riesterer L. and Jaques R. Pharmacology,2;1969:288–294 (DOI:10.1159/000136031)

  • Bolton EE, Wang Y, Thiessen PA and Bryant SH. PubChem: integrated platform of small molecules and biological activities. Annual reports in computational chemistry, 4;2008: 217-241.

  • Enroth, C., Eger, B.T., Okamoto, K., Nishino, T., Nishino, T., Pai, E.F. Crystal structure of xanthine oxidase from bovine milk, 10.2210/pdb1fiq/pdb, Deposited: 2000-08-04 Released: 2000-10-04.

  • Bernstein FC, Koetzle TF, Williams GJ, Meyer EF, Brice MD, Rodgers JR and Tasumi M. The Protein Data Bank: a computerbased archival file for macromolecular structures. Archives of biochemistry and biophysics, 185(2); 1978:584-591.

  • Thomsen R, and Christensen MH. MolDock: a new technique for high-accuracy molecular docking. Journal of medicinal chemistry,49(11); 2006: 3315-3321.

  • Gehlhaar DK, Bouzida D, Rejto PA. Fully automated and rapid flexible docking of inhibitors covalently bound to serine proteases. InInternational Conference on Evolutionary Programming 1998 Mar 25 (pp. 449-461). Springer, Berlin, Heidelberg.

  • Yang JM, and Chen CC. GEMDOCK: a generic evolutionary method for molecular docking. Proteins: Structure, Function, and Bioinformatics, 2004; 55(2): 288-304.

  • Lipinski CA. Lead-and drug-like compounds: the rule-of-five revolution. Drug Discovery Today: Technologies, 1(4); 2004:337-341.

  • Khan RA. Evaluation of flavonoids and diverse antioxidant activities of Sonchusarvensis. Chemistry Central Journal.6; 2012:126.


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  • In-Silico and in-Vitro Evaluation of Xanthine Oxidase Inhibition of Zingiber officinalae for Hypouricemic Activity

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Authors

Sivakumar Muthusamy
Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
Srikanth Jeyabalan
Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University) Chennai, India

Abstract


Zingiber officinale (ZO), traditionally known for its remedial potential, was examined on phytoconstituents analysis, antioxidant and xanthine oxidase inhibition by in-vitro analysis, followed by an in-silico study to identify the biological rationale. Results show that ethanolic extract of Zingiber officinale illustrated potential antioxidant to that of standard. DPPH ZO (IC50=42.09ug/mL), DPPH butylhydroxyltoluene (IC50=66.67ug/mL), nitric oxide ZO (IC50=28.36ug/mL), nitric oxide ascorbic acid (IC50=42.87𝜇g/mL). Zingiber officinale extract inhibited the xanthine oxidase activity (IC50 = 188.5ug/mL) compared to allopurinol (IC50=499.2ug/mL), which showed competitive inhibition. The in-silico docking studies of the major phytoconstituents revealed by GC-MS analysis from this extract suggest that the inhibitory activity may be due to the combined effect of these compounds. These results suggest substantial reason for the evaluation of active compounds in in-vivo system for the management and treatment of gout as well as in the oxidative stress conditions.

Keywords


Zingiber officinale, Anti-Oxidant assays, Molecular Docking, Hypouricemic.

References